Pmfbp1 (NM_019938) Mouse Untagged Clone

CAT#: MC223237

Pmfbp1 (untagged) - Mouse polyamine modulated factor 1 binding protein 1 (Pmfbp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pmfbp1
• Synonyms: 1700016D22Rik; F77; Stap
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223237 representing NM_019938
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAAAGCTTAAAGGAGAATTACGAACTGCCAAGGGACTGAAGAATGAGGCTGGGGAGAGAGACAGGG
  ATGTCAGCAATCTAAACAGCAAGCTCTTGAGCCTACAACTGGACATCAAGAATCTCCACGATGTCTGCAA
  GAGGCAGGGGAAGACCTTGCAAGAGAACCAGCTCTGCGTGGAGGAAGCGATGCTGAAAGCCAACCACAAC
  AAGAAGCAAGCCCAGACGTTAGTATTCACGGACAACCAGATGGACTTCCGGGTCAATAAGCAATACCATC
  TCAGGCAACTCCAGCAACTGAAGAAGAAGCTGCTGACCCTCCAGCAGGAACTGGAGTTCCGTACCCAGGA
  GCTCCAGGCTTCCTACTGCTCTCTCCTGCAGTACCAGTCGATCCTGGAGAAGCAGACTTCCGACCTTCTT
  GTTCTTCACCGTCACTGCAAACTTAAGGAAGATGAGGTCATTCTCTACGAGGAGGAGATGGGAAATCATA
  GTAAGAGCACGGGGGAGAAACTCCATCTGGCGCAAGAGCAGCTTGCCTTGGCGGGGGACAAGATCCTCTC
  CCTAGAAAGGAGCCTAAACCTCTACAGGGACAAGTACCAGACTTCCCTGAGCAACATAGAGTTACTGGAG
  TGCCAAGTGAAGATGCTGGAGGGGGAACTCAGCGGACTCATTGGTCAGGACCCTGAGAACAAAGGAGACC
  ACCCGAAGGTTCGAATCTACACTTCTCCGTGCGTGATTCAAGAGCATCAGGAGACCCTAAAGCGACTGTC
  TGAAGTCTGGCAAAAGGTTTCTGAACAGGATGACCTAATCCAGGAGCTTCGGAATAAACTGGCCTGCAGC
  AACTCTTTGGTCCTGGAGCGTGAGGAGGCGTTGATAAAATTACAAGCTGAGTTTGCTTCCTACACAGCCA
  CCCACCGGCATCCCCCTACCTCCTCAGAAGACTGTGAAGACATCACAAAGATCCTGAAGCACCTGCAAGA
  GCAGAAAGACAGCCAGTGCCTGCACGTGGAGGAGTACCAGAACCTTGTGAAGGACCTGCGCATGGAGCTG
  GAAGCTGTGTCAGAACAGAAGAAAAAGATCATGAAGGACATGATGAAGCTGGAGCTAGATCTACATGGGC
  TGCGGGAAGAGACGTCCTGCGTCATCGAGAAGAAGGACAAGGAGACTGTCTTCCTGCAGTACCGGCTGCA
  AGATCTGCAGCAACAGTACACAGAGTCACAGAAGCTGTCCCTGAAGAAGGACAAGTTGCTCCAAGACAAG
  GATGAGAGGCTGCACGAGCTGGAGAAGAATCTGATGCAGGTGCAGAACAGCCTCAGAGAGAAAGAGGCAG
  AGCTGGAGAAGCTACAGTGTACAACCAAAGAACTGGACACCAGCCTCCAAGAAGCTAGGCAAAGCACATC
  CAAAATAGACTGCGAGGCCCTGCGGGCCGAGATCCAGAAGCTCAAGGATAGCCTCGAGGAGGCCAGGGAG
  CAGCTGAAGGTGTCAGATCAGAATCTAACCCAGAGTAAGGAGGAAGCGCACCTCTCTGCCAGCAGCCTAG
  AGGATGCTCATCGGAAAATCGAGAACTGCCTTCTCCAGGACAAGCAGAAGGAAGAGGTCATTAAGGATCT
  GCAGAGCCAGTTGCATAAGCTACAGAAGGAGTCTTCGAAAATTGAGGAGGAACGAAAACACAACAGGCAA
  CGGTTACAGGAGCTGAGCTCGGAACTCTCTGAAGGCCAGAGAAGGCTTTCGAATGCAGAGAAGGAAAAGT
  CGCTGCTTCAGAAGACACTGGATGAAGACGAGAAAAAGATAGATGAGCTATTTCACAGCACCCAAGTCTC
  TGAGCAAAAGCAAAGGGAGCTAACAAACTCCATAAGGAAGCTAGAGGAGGAACTTCTGGAGATAAAAGGG
  CTCTTGGAGGAGAAACGAGAGCAACTGAAGAAGAGTAAGGAACAGGAGAAGGCACTGGAGGAAGAGATCG
  AGGCTTTGCGACAAGAAGCGAAAAGGAAAGAGAAGATGGCGAAGGAACATTTGAGAAAGCTGGATGAAGA
  GAAGGAGAACCTGCAGGCTGAGTTGACCTCCCGTTCCTCACACCTGGACTCCTCTCTCAACAAATACAAC
  TCCTCCCAGAAAGTCATCCAAGAGCTCAACGCGGAGATAGCCCGCCAGAAGGATTCCATCATGATTCTGC
  AGACACAGCTGGATTCGGCTATCCAGAAAGAGAAGAACTGTTTCCAGAACATGGTTAGTAAAGAAGCTTA
  TGAGGAACTTGTGCGGAAGTCGGGCAACTGCCAGGACGACCTGACACAGGCCCTGGAGAAGCTCACTCAG
  GCAACCTCAGAGACAAAGAGCCTGAATCGCTCCTTGCAACAGACCCAGGAGAGGAAAGCTCAACTGGAAG
  ATGAAATCGCGGCTTATGAGGAAAGGATGAAAAAGCTCAATATGGAGTTAAAAAAACTGCAGGGCTTCCA
  ACAGCAGAGCGAGCAAGAGGTGCACAACTTTGACAAGAAGCTGGAGGAGATGAGCAACCAGGTGTTGCAG
  TGGCAGAGGCAGCACCAGAGTGACCTTAAGATGTTGGCAGCTAAGGAGTCGCAGCTCCGAGAATTCCAGG
  AGGAAATGGCGACCCTAAGAGAGAGCCTCCTTGCAGATGAGAAGGAGCCGTACTCCCCGCCTGCAAAGCT
  AACCCCCAAAGAAACCTGCAGGCACCACCGGGAGAATGACCAGATTATGAACAACGTGGAACAATGGGCA
  AAGGAGCAGAAGATTGCCAATGAGAAGTTGGGAAACAAGCTGAGAGAGCAGGTTAAATACATTGCCAAGT
  TGACTGGCGAAAAGGACCACCTCCACAATGTAATGGTCCACCTGCAGCAGGAGAACAAAAAGCTGAAGAA
  CGAGATAGAAGAGAAGAAGCTAAAGGCCGGGAACCCGAGGATCTGTGTCAAGGCCTTTCCCCCAAACAAA
  CTGGAGCCTTCCCAAAAGGGAAAGCTATGCTGTGCCCTGGGCTGGAGAGGGATACCCCAGGACCTGACAC
  CGAAAACGGACCACAAGTACATGGGGTTACCTCACTCCTCAGGCTCCTCCTACTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019938
• Insert Size: 3069 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019938.3, NP_064322.1
• RefSeq Size: 3398 bp
• RefSeq ORF: 3069 bp
• Locus ID: 56523
• UniProt ID: Q9WVQ0
• Cytogenetics: 8 57.05 cM
• Gene Summary: Required for normal spermatogenesis (PubMed:30032984, PubMed:30298696). It functions as a scaffold protein that attaches the sperm head-tail connecting piece to the nuclear envelope, thus maintaining sperm head and tail integrity (PubMed:30032984). May also be involved in the general organization of cellular cytoskeleton.[UniProtKB/Swiss-Prot Function]