Magi1 (NM_001083321) Mouse Untagged Clone

CAT#: MC223233

Magi1 (untagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Magi1
• Synonyms: AIP3; Baiap1; BAP1; Gukmi1; Magi-1; MAGI1c; mKIAA4129; TNRC19; WWP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223233 representing NM_001083321
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAATGCTGGCATAGTCCACCCGGAGAATGAGGAGGAGGAGGATGTCCCTGAAATGAACAGTAGCT
  TTACAGCCGACTCTGGAGACCAGGACGAGCACACTCTCCAAGAAGCAACGCTCCCGCCTGTGAATAGTAG
  CATCCTCGCTGCTCCCATCACGGACCCTTCTCAGAAGTTCCCTCAGTACCTACCTCTTTCTGCAGAGGAT
  AATTTAGGTCCTCTACCTGAAAACTGGGAGATGGCCTATACTGAAAATGGAGAAGTCTATTTCATAGACC
  ACAACACGAAAACAACATCATGGTTAGACCCTCGGTGCCTGAACAAACAGCAGAAGCCTCTGGAAGAATG
  TGAAGATGATGAAGGGGTACACACCGAGGAGCTGGACAGTGAACTAGAGTTGCCTGCTGGCTGGGAAAAG
  ATTGAAGACCCTGTCTACGGTGTCTACTATGTAGACCACATCAACAGGAAGACGCAATATGAAAACCCAG
  TCCTAGAAGCCAAACGGAAGAAACAGCTTGAACAGCAGCAGCAACAGCAGCAGCCTCAGCCACCGCAGCC
  AGAAGAGTGGACAGAGGATCATGCATCTGTTGTGCCTCCTGTTGCTCCTTCCCATCCCCCGAGCAATCCG
  GAGCCAGCCAGGGAAACTCCACTTCAGGGCAAACCTTTTTTTACAAGAAACCCCTCTGAGCTGAAAGGCA
  AGTTCATTCACACGAAGCTACGGAAAAGCAGCCGAGGCTTTGGCTTCACGGTGGTTGGAGGAGACGAGCC
  TGATGAGTTCCTGCAGATCAAGAGCCTCGTCCTCGATGGTCCTGCCGCACTGGATGGCAAGATGGAGACA
  GGGGATGTAATTGTGAGTGTGAATGACACCTGTGTTTTGGGACACACACATGCTCAAGTTGTGAAAATCT
  TCCAGTCCATTCCCATTGGTGCCAGTGTGGACCTTGAACTCTGCAGAGGTTATCCATTGCCTTTTGACCC
  GGATGACCCTAATACAAGTTTAGTGACCTCGGTGGCCATTTTGGACAAAGAACCAATTATTGTAAATGGA
  CAAGAGACCTACGATTCACCAGCGAGCCACAGTAGTAAAACAGGCAAAGTCAGCAGCATGAAGGATGCCA
  GGCCAAGCAGCCCTGCTGATGTGGCTTCCAACAGCTCTCATGGTTATCCCAACGACACAGTCTCCTTGGC
  TTCCTCCATAGCCACCCAGCCAGAGCTAATAACTGTTCACATAGTCAAAGGGCCAATGGGATTTGGCTTT
  ACGATCGCAGACAGTCCCGGTGGGGGTGGCCAAAGAGTGAAACAGATTGTTGACAGTCCACGCTGCAGAG
  GCCTCAAAGAAGGGGATCTTATCGTGGAGGTGAATAAGAAGAACGTGCAGGCCCTGACGCACAATCAAGT
  CGTGGATATGCTGATTGAATGTCCCAAGGGAAGTGAGGTCACACTGTTGGTGCAGCGAGGAGGGCTACCA
  GTTCCCAAGAAGAGCCCAAAGTCGCAGCCACTGGAGAGGAAAGACAGCCAGAATAGCTCCCAGCACAGCG
  TCTCCAGCCACCGGAGCCTGCACACTGCGTCCCCGAGCCACGGCATACAGGTGCTCCCTGAGTACCTACC
  TGCAGACGCCCCTGCTCCAGATCAGACCGACAGCTCTGGGCAGAAAAAGCCAGATCCTTTTAAAATCTGG
  GCCCAGTCCAGGAGCATGTATGAAAACCGACCTATGTCACCTTCGCCTGCATCAGGATTGAGCAAGGGTG
  AAAGAGACAGAGAAATCAATTCCACGAATTTTGGAGAATGTCAGATTCCAGATTACCAGGAACAGGACAT
  CTTCCTCTGGAGAAAAGAAACCGGATTTGGATTTAGGATTCTGGGTGGAAATGAACCAGGGGAACCCATT
  TATATCGGTCACATCGTACCGCTGGGTGCTGCTGACACAGACGGCCGCCTGAGGTCTGGAGATGAATTAA
  TCTGTGTGGATGGGACACCAGTAATTGGGAAATCACACCAGCTCGTGGTCCAGCTTATGCAACAAGCTGC
  CAAGCAAGGCCATGTCAATCTCACAGTGAGGCGGAAAGTGGTCTTTGCCGTCCCCAAAGCAGAGAATGAG
  GTGCCCTCACCAGCCTCATCACACCACAGTAGCAACCAGCCCGCGTCCCTGACGGAGGAGAAACGCACAC
  CGCAAGGCAGCCAGAACTCTCTGAACACTGTGAGCTCTGGCAGCGGCAGCACCAGTGGCATTGGCAGTGG
  TGGCGGCGGGGGCAGCGGTGTGGTGAGCGCTGTGCTGCAGCCCTATGATGTGGAGATTCGGCGTGGGGAG
  AACGAGGGCTTTGGGTTTGTCATCGTGTCCTCCGTGAGCAGACCCGAAGCGGGCACAACCTTCGGCAATG
  CATGTGTGGCTATGCCTCACAAAATAGGTCGGATTATTGAGGGGAGCCCTGCTGACCGCTGTGGCAAGCT
  GAAAGTAGGAGACCGGATCTTGGCAGTAAATGGATGTTCCATCACCAACAAATCCCATTCTGACATTGTC
  AACCTAATCAAAGAAGCGGGCAACACAGTGACTCTCCGCATCATCCCCGGGGATGAGTCCTCAAATGCCA
  CGCTGCTGACTAATGCTGAGAAGATTGCCACCATCACCACCACTCATGCCCCCTCTCAGCAGGGGACCCA
  GGAAACAAGGACCACCACCAAACCAAAGCAGGATTCTCAGTTTGAGTTCAAAGGACCGCAGGCTGCACAG
  GAGCAAGATTTCTACACTGTGGAATTGGAAAGAGGGGCCAAGGGATTTGGCTTTAGTCTTCGAGGGGGCC
  GAGAATATAACATGGATCTTTATGTTCTGCGCTTGGCAGAGGATGGTCCTGCAGAAAGATGTGGGAAGAT
  GAGGATTGGCGATGAAATTCTAGAGATCAATGGTGAGACCACCAAAAACATGAAACACTCTCGGGCCATA
  GAACTGATCAAGAATGGCGGCCGCAGGGTCCGTCTGTTTCTGCGGCGGGGAGACGGCTCAGTCCCAGAAT
  ATGGTGGGTCAAACTATGAAAACATCCCTTCCTTCCCTGGCATGACTCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001083321
• Insert Size: 3063 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083321.2, NP_001076790.1
• RefSeq Size: 5105 bp
• RefSeq ORF: 3063 bp
• Locus ID: 14924
• Cytogenetics: 6 D1
• Gene Summary: May play a role as scaffolding protein at cell-cell junctions. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) has multiple differences, compared to variant 3. These differences cause translation initiation at a downstream start codon and result in a different 3' coding region and 3' UTR, compared to variant 3. It encodes isoform d, which is shorter and has a distinct C-terminus, compared to isoform c.