Atp4a (NM_018731) Mouse Untagged Clone

CAT#: MC223230

Atp4a (untagged) - Mouse ATPase, H+/K+ exchanging, gastric, alpha polypeptide (Atp4a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp4a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223230 representing NM_018731
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGGGAAGGCAGAGAATTATGAACTGTACTCAGTGGAACTGGGGTCTGGCCCTGGTGGGGACATGACTG
  CCAAGATGAGCAAGAAGAAGGCAGGAGGTGGGGGAGGCAAGAAGAAGGAGAAGCTGGAGAACATGAAGAA
  GGAGATGGAGATTAACGACCACCAGCTATCAGTGTCTGAGCTGGAGCAGAAGTATCAGACCAGCGCCACC
  AAGGGCCTGAAGGCGAGCCTGGCAGCTGAGCTGCTGCTGAGGGATGGACCCAATGCGCTCCGGCCACCTC
  GGGGCACTCCCGAGTATGTGAAGTTTGCCCGTCAGCTGGCAGGTGGTCTGCAGTGCCTCATGTGGGTGGC
  TGCTGCTATCTGCCTCATTGCCTTTGCCATCCAGGCCAGCGAGGGTGACCTGACCACTGATGATAATCTG
  TACCTGGCGGTGGCACTCATTGCTGTGGTTGTGGTCACTGGCTGTTTTGGCTACTACCAGGAGTTCAAGA
  GCACAAATATCATCGCCAGCTTCAAGAATCTTGTACCCCAGCAAGCCACTGTGATCCGAGACGGGGACAA
  GTTCCAGATCAACGCGGATCAGCTTGTGGTGGGCGACCTGGTGGAGATGAAAGGCGGGGACCGCGTGCCA
  GCAGACATCCGCATTCTGTCAGCCCAGGGCTGCAAGGTGGACAACTCCTCGCTCACTGGAGAGTCTGAAC
  CGCAGACCCGCTCGCCCGAGTGTACACATGAGAGTCCCCTTGAGACCCGCAACATCGCCTTCTTCTCCAC
  CATGTGTCTGGAGGGAACAGCTCAGGGTTTGGTGGTGAGCACCGGCGATCGCACCATCATTGGGCGCATC
  GCCTCGCTGGCCTCGGGTGTGGAAAACGAGAAGACTCCGATTGCAATCGAGATTGAACATTTTGTGGACA
  TCATTGCCGGCCTGGCCATCCTCTTCGGTGCCACATTCTTTGTGGTGGCCATGTGTATTGGCTATACCTT
  CCTTCGGGCCATGGTCTTCTTCATGGCCATTGTGGTAGCCTATGTGCCTGAGGGGCTGCTGGCAACTGTC
  ACAGTCTGCCTGTCACTGACAGCAAAGAGGTTGGCCAGTAAAAACTGCGTGGTTAAAAACTTAGAAGCAG
  TTGAGACCCTAGGTTCTACATCAGTCATCTGCTCAGACAAGACAGGAACTCTCACTCAGAACCGCATGAC
  GGTGTCTCATCTATGGTTTGACAACCATATCCACACGGCGGACACCACAGAAGACCAGTCAGGGCAAACG
  TTCGATCAGTCGTCGGAGACATGGCGAGCGCTGTGCCGCGTGCTCACCCTGTGCAACCGCGCTGCCTTCA
  AGTCCGGCCAGGACGCGGTGCCGGTGCCCAAGCGCATCGTGATCGGAGACGCGTCAGAGACTGCGCTGCT
  CAAGTTTTCGGAGCTGACGTTGGGCAACGCCATGGGTTACAGGGACCGCTTCCCCAAAGTTTGCGAGATC
  CCCTTCAACTCCATCAACAAGTTCCAGCTGTCCATTCATACCCTGGAGGATCCACGCGACTCCCGGCACC
  TGCTGGTGATGAAGGGCGCCCCTGAGCGCGTGCTGGAGCGTTGCAGCTCCATCCTCATCAAGGGCCAGGA
  GCTGCCTCTGGACGAGCAATGGCGTGAGGCCTTCCAGACAGCCTACCTCAGCCTGGGAGGCCTAGGCGAA
  CGTGTTCTTGGTTTCTGCCAGCTCTACCTGAATGAGAAGGACTACCCACCTGGCTATGCCTTTGATGTGG
  AGGCCATGAACTTTCCGAGTAGTGGCCTCTGCTTTGCGGGACTTGTATCCATGATTGACCCTCCCCGGGC
  CACCGTTCCTGATGCTGTGCTCAAATGCCGCACGGCAGGCATCCGGGTGATCATGGTGACCGGTGACCAT
  CCCATCACGGCCAAGGCCATCGCAGCCAGCGTGGGTATCATCTCAGAGGGCAGCGAGACAGTGGAAGACA
  TCGCCGCCCGCCTCCGGATGCCGGTGGACCAGGTTAATAGGAAGGATGCCCGGGCCTGCGTGATCAATGG
  TATGCAGCTGAAGGACATGGACCCGTCTGAGCTGGTGGAGGCGCTACGCACCCACCCTGAGATGGTGTTT
  GCTCGAACCAGTCCTCAGCAGAAGCTGGGTGCCATTGTGGCTGTAACAGGGGATGGTGTGAATGACTCCC
  CAGCCCTGAAGAAAGCTGACATTGGTGTAGCCATGGGCATTGCTGGCTCGGATGCTGCTAAAAATGCTGC
  TGACATGATCTTGCTGGATGACAACTTTGCTTCCATTGTGACAGGCGTGGAGCAGGGCCGACTGATCTTT
  GACAACCTGAAGAAATCCATTGCATATACACTGACCAAGAACATTCCAGAACTGACACCCTACCTTATCT
  ATATCACTGTCAGTGTGCCCCTACCCCTTGGGTGTATCACCATTCTCTTCATAGAACTCTGCACAGATAT
  CTTTCCATCTGTGTCCCTGGCGTACGAGAAGGCTGAGAGCGACATCATGCACCTGCGCCCACGGAACCCT
  AAGCGTGACCGGTTGGTCAACGAACCCCTGGCTGCTTATTCCTATTTCCAGATTGGTGCCATTCAGTCAT
  TTGCCGGCTTTGCTGACTACTTCACGGCCATGGCGCAGGAGGGCTGGTTCCCTCTGTTGTGTGTAGGACT
  GCGGCCACAATGGGAGGACCACCATCTACAAGATCTTCAAGACAGCTACGGCCAGGAATGGACATTTGGT
  CAGCGCCTGTACCAGCAGTACACCTGTTACACCGTGTTCTTCATCAGCATCGAGATGTGCCAGATCGCAG
  ATGTCCTCATCCGCAAGACACGGCGCCTCTCTGTTTTCCAGCAGGGATTCTTCAGGAACAAGATCCTGGT
  GATCGCCATCGTGTTCCAGGTCTGCATTGGCTGCTTCCTGTGCTACTGCCCAGGGATGCCTAACATCTTC
  AACTTCATGCCCATCCGGTTCCAGTGGTGGCTGGTCCCCATGCCCTTCGGCCTTCTCATCTTTGTCTATG
  ATGAGATCCGGAAACTTGGAGTTCGCTGTTGCCCAGGGAGCTGGTGGGACCAGGAACTCTACTATTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: AscI-RsrII
• ACCN: NM_018731
• Insert Size: 3078 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018731.3, NP_061201.2
• RefSeq Size: 3470 bp
• RefSeq ORF: 3078 bp
• Locus ID: 11944
• Cytogenetics: 7 19.2 cM