Trhde (NM_146241) Mouse Untagged Clone

CAT#: MC223229

Trhde (untagged) - Mouse TRH-degrading enzyme (Trhde), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trhde
• Synonyms: 9330155P21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223229 representing NM_146241
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAAGACGACGCCGCGCTCCGGGCAAGCGGCAGGGGGCTCTCGGACCCGTGGGCTGACTCGGTGG
  GAGTGAGACCCCGCACCACGGAGCGCCACATCGCAGTGCACAAGAGGCTTGTGCTGGCCTTTGCCGTGTC
  CATCGTGGCACTGCTGGCTGTCACCATGCTGGCCGTCCTGCTCAGCTTGCGATTCGACGAGTGTGGAGCG
  AGCGCGGCGATGCCTGGCACCGACGGTGGCCTCGGGGGTTTCCCCGAGCGCGACAGCAACAGCAGCTTTC
  CAGGTTCTGCCCGGCGCAACCACCACGCGGGTGGGGAATCCTCTCAGCGTGAGAGCGGCGAGGTGGGCAC
  CCCGGGGACCCCGTCTGCCCAGCCGCCGTCGGAGGAAGAGCGGGAGCAGTGGCAGCCCTGGACACAGCTG
  CGCCTATCCGGCCACCTCAAGCCGCTGCACTACAATTTGATGCTCACCGCTTTCATGGAGAACTTCACCT
  TCTCTGGGGAGGTCAACGTGGAGATCGCGTGCCGGAACGCCACCCGCTACGTGGTACTGCACGCCTCCCG
  AGTGGCGGTGGAGAAGGTGCAAGTAGCTGAAGACAGGGCGTTCGGGGCTGTCCCGGTTGCAGGCTTTTTC
  CTCTACCCACAAACGCAGGTCTTGGTGGTGGTATTGAACAGAACCCTGGACGCGCAGAGGCATTACAATC
  TGAAGATTATCTACAATGCCCTGATAGAGAACGAGCTTTTGGGCTTCTTCCGCAGCTCCTACGTGATCCA
  CGGGGAGAGAAGATTCCTTGGTGTTACTCAGTTTTCACCTACACATGCCAGGAAGGCGTTTCCGTGTTTC
  GATGAACCAATCTACAAGGCCACTTTCAAAATCAGCATCAAACATCAAGCAACCTATTTGTCTCTATCCA
  ACATGCCAGTGGAAACGTCTGTGTTCGAGGAAGATGGATGGGTGACAGATCACTTTTCACAGACCCCTCT
  CATGTCTACATATTATTTAGCCTGGGCGATTTGCAACTTCACATACAGAGAAACTACCACCAAGAGTGGG
  GTTGTAGTCCGATTATATGCGAGACCCGACGCTATCAGAAGAGGATCCGGGGACTATGCTCTCCACATTA
  CAAAGAGATTAATAGAATTTTATGAAGACTACTTTAAAGTGCCCTATTCTTTGCCAAAACTAGATCTTTT
  AGCTGTGCCTAAGCATCCTTATGCTGCTATGGAGAACTGGGGACTAAGTATTTTTGTGGAACAAAGAATA
  CTGCTGGATCCCAGTGTGTCATCTATTTCTTATTTGCTGGATGTCACCATGGTCATTGTTCATGAAATAT
  GTCACCAGTGGTTTGGTGACCTTGTGACTCCAGTTTGGTGGGAAGATGTGTGGTTGAAGGAAGGCTTTGC
  TCACTACTTTGAATTTGTGGGTACAGACTACCTCTACCCTGCCTGGAACATGGAGAAGCAGAGATTTCTG
  ACAGATGTTCTGCATGAAGTGATGCTACTTGATGGCTTGGCCAGTTCCCATCCAGTATCACAGGAAGTGC
  TACGGGCAACAGATATTGACAGAGTGTTCGACTGGATCGCATACAAAAAGGGTGCTGCTCTAATTAGAAT
  GCTGGCTAATTTTATGGGTCATTCAGTTTTTCAGAGGGGATTGCAAGATTATTTAACCATTCACAAGTAT
  GGCAATGCAGCCAGAAATGATCTCTGGAATACATTATCAGAGGCTTTAAGAAGGAATGGAAAATACGTGA
  ACATACAAGAAGTAATGGATCAGTGGACACTGCAGATGGGATATCCTGTTATCACTATCCTGGGAAACAC
  GACGGCAGAAAATAGAATACTTATCACCCAACAGCATTTCATTTATGACATTGGTGCTAAAACTAAAGCA
  CTTCAACTTCAAAACAGCAGTTACCTGTGGCAGATTCCATTAACCATTGTGGTAGGAAATAGAAGCCATG
  TGTCTTCAGAAGCAATTATTTGGGTGTCTAACAAATCAGAACATCACAGAATAGCGTACTTAGACAGAGG
  AAGCTGGATCCTTGGGAACATCAATCAAACCGGCTACTTTAGAGTCAACTATGACCTAAGGAACTGGAGA
  TTACTAATCGATCAGTTAATCAGGAACCACGAGGTACTTTCTGTCAGTAACCGCGCGGCTTTGATCGATG
  ATGCCTTCAGCCTGGCCAGGGCAGGCTATTTGCCTCAGAATATCCCCCTAGAGATCATCAGATACCTGTC
  TGAGGAAAAGGATTTTCTTCCTTGGCACGCTGCCAGCCGAGCTCTTTATCCTCTGGATAAATTACTGGAC
  CGCATGGAGAACTACAATATCTTCAATGAATATATTCTAAAACAAGTTGCAACAACATACATCAAGCTTG
  GATGGCCAAGAAATAACTTCAATGGATCTCTCGTTCAAGCCTCCTACCAACATGAGGAGCTACGGAGGGA
  AGTGATAATGTTGGCATGTAGTTTTGGGAACAAGCACTGTCACCAGCAGGCATCAACACTTATTTCAGAC
  TGGATTTCCAGCAACAGAAACAGAATACCCCTGAATGTTAGAGACATCGTCTACTGCACAGGAGTGTCAC
  TGCTGGATGAGGATGTCTGGGAGTTTATCTGGATGAAATTCCACTCTACCACTGCAGTTTCTGAGAAAAA
  GATACTGCTGGAGGCTCTAACTTGCAGCGATGACAGGAATTTATTAAGTAGGCTTCTAAATCTGTCTCTG
  AATTCTGAGGTGGTGTTGGATCAAGATGCAATTGATGTAATTATCCACGTAGCCAGAAATCCACATGGCC
  GAGACCTTGCCTGGAAGTTTTTCAGAGACAAATGGAAAATACTAAATACCAGGTATGGAGAAGCACTGTT
  TATGAATTCCAAACTTATCAGTGGAGTTACAGAATTTCTTAATACAGAAGGTGAACTTAAAGAGCTAAAG
  AATTTCATGAAGTCCTATGATGGGGTAGCCTCTGCTTCTTTCTCACGAGCTGTGGAAACTGTGGAAGCCA
  ATGTGCGCTGGAAAAGGTTTTACCAAGATGAGCTTTTCCAGTGGCTGGGAAAAGCCATGAGACACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146241
• Insert Size: 3078 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146241.2, NP_666353.1
• RefSeq Size: 5722 bp
• RefSeq ORF: 3078 bp
• Locus ID: 237553
• UniProt ID: Q8K093
• Cytogenetics: 10 D2
• Gene Summary: Specific inactivation of TRH after its release.[UniProtKB/Swiss-Prot Function]