Nod2 (NM_145857) Mouse Untagged Clone

CAT#: MC223222

Nod2 (untagged) - Mouse nucleotide-binding oligomerization domain containing 2 (Nod2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nod2
• Synonyms: ACUG; BLAU; Card15; CD; F830032C23Rik; IBD1; Nlrc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223222 representing NM_145857
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTCACAGGAAGAGTTCCAGGCACAGAGGAGCCAGCTGGTGGCACTGCTGATCTCAGGGTCCTTGG
  AGGGCTTTGAGAGCATCTTAGACTGGCTGCTGTCTTGGGATGTGCTCTCCAGGGAGGACTATGAGGGCCT
  CAGCCTCCCTGGCCAACCTCTCTCCCATTCTGCCAGGCGTCTGCTGGACACAGTCTGGAACAAGGGTGTT
  TGGGGCTGTCAGAAGCTCCTCGAAGCTGTGCAGGAGGCACAGGCCAACAGTCATACCTTTGAACTGTATG
  GGTCCTGGGACACTCACTCCCTTCATCCAACCAGAGACCTGCAGAGTCACCGGCCAGCCATTGTGAGGAG
  ACTCTACAACCATGTAGAAGCCATGCTGGAGCTGGCAAGGGAGGGGGGGTTTCTGAGCCAGTACGAGTGT
  GAGGAGATCAGGCTGCCAATCTTCACGTCGTCTCAGAGGGCAAGAAGGCTGCTTGATCTCGCTGCGGTGA
  AGGCCAATGGACTGGCTGCCTTCCTTCTACAGCACGTCAGGGAACTACCAGCTCCACTGCCTCTGCCTTA
  CGAGGCTGCTGAGTGTCAGAAGTTCATATCTAAGCTGAGGACCATGGTGTTGACTCAGTCTCGCTTCCTC
  AGTACTTACGATGGGTCGGAGAATCTTTGCCTGGAGGATATATACACGGAGAACATCTTGGAGCTGCAGA
  CTGAAGTGGGCACAGCCGGGGCCTTGCAGAAGAGCCCTGCCATCCTGGGCCTGGAGGACCTCTTTGATAC
  CCATGGTCACCTGAACAGAGATGCCGACACCATACTGGTGGTGGGCGAAGCAGGCAGTGGCAAGAGCACT
  CTTCTGCAGCGTTTGCACCTGCTGTGGGCAACAGGGAGGAGCTTCCAGGAGTTTCTCTTCATTTTCCCAT
  TCAGCTGCCGACAGTTGCAATGCGTGGCCAAACCGCTGTCCCTGAGGACGCTGCTCTTTGAGCACTGCTG
  CTGGCCTGATGTCGCTCAGGACGATGTCTTCCAGTTCCTTCTTGACCATCCTGACCGTGTCCTGTTAACC
  TTTGATGGCTTGGACGAGTTCAAGTTCCGGTTCACCGACCGGGAGCGCCACTGCTCTCCAATTGACCCCA
  CGTCAGTCCAGACTCTGCTCTTCAACCTTCTCCAGGGGAACCTGCTGAAGAATGCCTGCAAGGTGCTGAC
  CAGCCGTCCGGATGCTGTGTCAGCGCTCCTCAGGAAGTTCGTTCGCACAGAGTTGCAACTGAAGGGCTTC
  TCTGAAGAGGGCATCCAACTGTACCTGAGAAAGCACCACCGGGAACCTGGGGTGGCAGACCGCCTCATCC
  AGCTGATCCAAGCCACCTCAGCCCTGCATGGTTTGTGCCACCTCCCTGTCTTCTCTTGGATGGTGTCCAG
  ATGCCACCGGGAACTTCTGCTGCAGAACAGGGGCTTCCCAACAACCAGCACGGACATGTACCTCCTGATC
  CTGCAGCATTTCCTGCTGCATGCCTCCCCTCCGGACTCCTCCCCACTCGGTCTGGGACCTGGACTCCTCC
  AAAGCCGGCTCTCCACCCTCCTGCACCTTGGCCACCTGGCTCTCCGGGGCCTGGCCATGAGCTGCTATGT
  GTTCTCAGCCCAGCAGCTCCAGGCAGCTCAGGTTGACTCTGATGATATTTCTCTTGGTTTCCTGGTACGT
  GCCCAAAGTAGTGTGCCCGGGAGCAAGGCGCCCCTGGAGTTCCTGCACATTACCTTCCAGTGCTTTTTTG
  CCGCTTTCTACTTGGCTGTCAGTGCTGACACATCGGTGGCCTCTCTCAAGCACCTTTTCAGCTGTGGCCG
  GCTGGGCAGCTCACTGCTGGGAAGGCTGCTGCCCAACCTGTGTATCCAGGGCTCCAGAGTCAAGAAGGGC
  AGCGAAGCAGCCCTGCTGCAGAAGGCTGAGCCACACAACCTGCAAATCACAGCAGCCTTCCTAGCAGGTC
  TGTTGTCCCAGCAGCATCGGGACCTGTTGGCTGCATGCCAGGTCTCCGAGAGGGTACTGCTCCAGCGTCA
  GGCACGTGCCCGCTCGTGTCTGGCCCACAGCCTCCGCGAGCACTTCCATTCCATCCCGCCTGCCGTGCCC
  GGTGAGACCAAGAGCATGCATGCTATGCCGGGCTTCATTTGGCTCATCCGTAGCCTGTACGAGATGCAGG
  AGGAGCAGTTGGCCCAGGAGGCTGTCCGTCGCTTGGACATCGGGCACCTGAAGTTGACATTTTGCAGAGT
  GGGCCCTGCAGAGTGTGCTGCACTGGCCTTTGTACTGCAACATCTCCAGCGGCCTGTGGCCCTACAGCTG
  GATTACAACTCTGTGGGAGATGTTGGAGTGGAACAGCTGCGACCGTGCCTTGGGGTCTGCACAGCTCTGT
  ATTTGCGAGATAACAATATCTCAGACCGAGGTGCCCGCACGCTGGTTGAGTGTGCTCTTCGCTGTGAGCA
  GCTGCAGAAACTAGCTCTCTTCAACAACAAACTCACGGATGCGTGCGCCTGCTCCATGGCCAAGCTCCTT
  GCACACAAGCAGAACTTCTTGTCCCTGAGGGTGGGGAACAATCACATCACAGCCGCTGGAGCCGAGGTGC
  TGGCCCAGGGACTCAAGAGCAACACCTCCCTGAAGTTCCTGGGGTTCTGGGGCAACAGCGTGGGTGATAA
  GGGCACCCAAGCCCTGGCTGAAGTTGTAGCCGACCACCAGAACCTAAAGTGGCTCAGCCTGGTAGGAAAC
  AACATTGGCAGCATGGGTGCCGAAGCCCTAGCACTGATGCTGGAGAAGAACAAGTCACTAGAGGAACTCT
  GCCTAGAGGAAAACCATATCTGTGACGAAGGGGTATATTCTCTCGCAGAAGGACTCAAGAGAAATTCAAC
  TTTGAAATTCTTGAAACTGTCCAACAATGGCATCACCTACCGGGGTGCAGAAGCCCTCCTGCAGGCCCTC
  AGCAGGAACAGTGCCATTCTGGAGGTTTGGCTTCGAGGGAACACATTCTCTTTGGAGGAAATCCAAACAC
  TGAGCTCCAGGGACGCCAGACTCTTGTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145857
• Insert Size: 3042 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145857.2, NP_665856.2
• RefSeq Size: 4621 bp
• RefSeq ORF: 3042 bp
• Locus ID: 257632
• Cytogenetics: 8 C3