Ide (NM_031156) Mouse Untagged Clone

CAT#: MC223204

Ide (untagged) - Mouse insulin degrading enzyme (Ide), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ide
• Synonyms: 1300012G03Rik; 4833415K22Rik; AA675336; AI507533
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223204 representing NM_031156
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAACGGGCTCGTGTGGCTGCTGCACCCCGCGCTGCCCGGCACCTTGCGCTCCATCCTCGGCGCCC
  GCCCGCCGCCCGCGAAGCTACTGTGTGGATTCCCAAAACAGACTTACAGCACAATGAGTAATCCGGCCAT
  CCAGAGAATAGAAGACCAAATTGTCAAGTCTCCTGAAGACAAACGGGAATACCGTGGACTAGAGCTGGCC
  AATGGCATCAAAGTGCTTCTCATCAGCGATCCCACCACAGACAAGTCCTCAGCGGCCCTCGATGTGCACA
  TAGGTTCACTGTCAGACCCTCCAAATATTCCTGGCTTAAGTCATTTTTGTGAACATATGCTGTTTTTGGG
  AACCAAGAAATATCCTAAAGAAAATGAATATAGCCAGTTTCTCAGTGAACATGCTGGAAGTTCAAATGCA
  TTCACCAGTGGAGAACACACCAATTATTATTTCGATGTTTCCCATGAACACTTGGAAGGAGCCCTGGACA
  GGTTTGCGCAGTTTTTCCTGTGCCCCTTGTTTGATGCAAGTTGTAAAGACAGAGAGGTGAACGCTGTCGA
  TTCAGAACATGAGAAGAATGTGATGAACGATGCCTGGAGACTCTTCCAGCTGGAAAAGGCTACGGGGAAC
  CCCAAACACCCCTTCAGCAAATTTGGGACAGGAAACAAATATACTCTAGAGACTCGGCCCAACCAAGAAG
  GCATCGACGTAAGGGAAGAGCTCTTGAAATTTCACTCTACGTATTATTCGTCCAATCTGATGGCGATTTG
  TGTTTTAGGTCGAGAATCCTTAGACGACCTCACTAACCTGGTGGTGAAGCTGTTCTCCGAGGTGGAGAAC
  AAGAACGTTCCTCTGCCTGAGTTCCCTGAGCACCCTTTCCAGGAAGAGCATCTCAGGCAACTTTATAAAA
  TAGTACCCATTAAGGATATTAGAAATCTTTATGTGACATTTCCCATACCAGACCTTCAGCAGTACTACAA
  ATCCAATCCTGGTCATTATCTTGGTCATCTAATTGGGCACGAAGGTCCTGGAAGCCTGTTGTCAGAACTC
  AAATCAAAGGGCTGGGTGAATACCCTGGTTGGTGGACAGAAGGAAGGAGCCCGAGGTTTTATGTTTTTTA
  TCATTAATGTGGACTTAACTGAAGAAGGGTTATTGCACGTTGAAGATATAATTTTGCACATGTTTCAATA
  CATTCAGAAGCTACGTGCAGAAGGACCTCAAGAATGGGTTTTCCAAGAGTGCAAGGACTTGAATGCTGTT
  GCTTTCAGATTTAAAGATAAAGAGAGACCACGAGGCTATACGTCCAAGATTGCAGGAAAGTTGCACTATT
  ATCCCCTAAATGGAGTGCTCACAGCTGAATATCTATTGGAGGAATTTAGACCCGACTTGATAGACATGGT
  TCTTGATAAACTCAGACCAGAAAATGTGCGGGTGGCAATAGTTTCCAAATCATTTGAAGGGAAAACTGAC
  CGCACAGAGCAGTGGTATGGAACACAGTATAAGCAAGAAGCTATCCCAGAGGACATCATTCAGAAATGGC
  AAAATGCTGACCTTAATGGAAAATTTAAGCTTCCAACAAAGAATGAATTTATTCCCACAAATTTTGAGAT
  TTTGTCATTAGAAAAAGATGCAACACCATACCCTGCTCTTATTAAGGATACAGCCATGAGTAAGCTGTGG
  TTCAAACAAGATGATAAATTTTTCTTGCCGAAAGCTTGTCTCAACTTTGAATTTTTCAGCCCGTTTGCTT
  ATGTGGACCCCTTGCACTGTAACATGGCCTATTTGTACCTTGAGCTCCTCAAAGACTCACTCAACGAGTA
  TGCATATGCAGCAGAGCTAGCAGGCCTGAGCTATGACCTCCAAAATACCATCTATGGGATGTATCTCTCA
  GTGAAAGGTTACAATGACAAACAGCCAATTTTGCTGAAGAAGATCACCGAGAAAATGGCTACTTTTGAGA
  TTGATAAGAAGAGATTTGAAATTATCAAAGAGGCCTATATGCGATCTCTTAATAACTTCCGGGCTGAGCA
  GCCTCACCAGCATGCCATGTACTACCTCCGCTTGCTGATGACTGAAGTGGCCTGGACTAAAGATGAGCTA
  AAAGAAGCCCTTGACGATGTTACCCTCCCCCGACTTAAGGCCTTCATACCTCAGCTGCTGTCACGGCTGC
  ATATTGAAGCACTTCTCCATGGAAACATAACCAAGCAGGCTGCCTTAGGAGTTATGCAGATGGTAGAAGA
  CACTCTTATCGAGCATGCTCACACCAAACCTCTCCTTCCAAGTCAGCTAGTCCGGTACAGAGAAGTCCAG
  CTCCCTGACAGAGGATGGTTTGTTTATCAGCAGAGGAATGAAGTTCACAATAACTGCGGCATCGAGATCT
  ACTACCAGACAGACATGCAGAGCACCTCGGAGAACATGTTCCTGGAGCTGTTTTGCCAGATTATCTCGGA
  GCCTTGCTTCAATACCCTGCGCACCAAGGAGCAGCTTGGCTATATTGTCTTCAGTGGACCCCGTCGGGCC
  AACGGTATCCAAGGCTTGCGCTTCATCATCCAGTCAGAAAAACCACCCCACTACCTGGAGAGCAGAGTAG
  AAGCCTTCTTGATTACCATGGAAAAGGCCATAGAGGACATGACAGAGGAGGCGTTCCAAAAACACATTCA
  AGCACTAGCGATTCGGCGACTCGACAAACCAAAGAAACTCTCTGCGGAGTGTGCTAAATACTGGGGGGAA
  ATCATCTCGCAGCAGTACAATTACGACAGAGATAACATAGAAGTTGCATATTTAAAGACACTTACCAAGG
  ACGATATCATCAGATTCTACCAGGAAATGTTGGCTGTCGACGCACCGAGGAGACATAAAGTGTCTGTCCA
  TGTTCTTGCCAGGGAAATGGATTCTTGTCCTGTTGTTGGAGAGTTCCCCTCTCAGAATGATATAAACTTG
  TCCGAAGCGCCACCCTTGCCACAACCTGAAGTGATTCACAACATGACTGAGTTCAAGCGTGGCCTGCCAC
  TGTTTCCCCTCGTGAAACCACATATTAACTTCATGGCTGCAAAACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031156
• Insert Size: 3060 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031156.3, NP_112419.3
• RefSeq Size: 5087 bp
• RefSeq ORF: 3060 bp
• Locus ID: 15925
• Cytogenetics: 19 32.24 cM