Espnl (NM_001033292) Mouse Untagged Clone

CAT#: MC223185

Espnl (untagged) - Mouse espin-like (Espnl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Espnl
• Synonyms: Gm556
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223185 representing NM_001033292
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCAGCAGGCACTGGTGGCCTCCAAGGATGGGGATATGGCCACCTTGGAACGGCTATTTGAGG
  CTGGTGCCCTAAGACCAGACATCACTGATGATCTGGGGGCTGGCCTGGTGCATCATGCCACCCGGGCTGG
  TCACCTGGACTGTGTCAAGTTCCTGGTACAGAGAGCCAAGCTGCCAGGCAACCAGCAGGCACACAACGGG
  GCCACACCAGTGCACGATGCAGCAGCCACGGGCAACCTGGCAGAGCTGTGCTGGCTGGTCCGTGATGCGG
  GCTGCGGGCTGCAGGACCAAGATGCCTCAGGTGTCTCTCCGCTGCACCTGGCTGCCCGGTTTGGACACCC
  AGCCCTGGTGGAGTGGTTACTCCGTGAGGGCCATGCAGCCACACTGGAGACGCTGGAAGGGGCCTTACCA
  CTGCACCACGCTGCTGTAAGTGGTGATCTGACCTGTCTGAAACTCCTGACAGCGGCCCACAGCAGTGGTG
  TGAACCAGCGGACATGCAGTGGTGCCTCTCCACTCTACCTGGCCTGCCAAGAGGGCCACCTGCATCTGGC
  ACAGTTCCTGGTGAAAGACTGCGGGGCCGACGTGCGCCTGAGAGCCCTGGATGGCATGAGCTCATTGCAC
  GCTGCGGCTGCCCACGGCCACTACTCTCTAGTTGTCTGGCTGGTGACATTCACAGACATCGGCCTGACAG
  CACGGGATAATGAAGGAGCCACTGCCCTGCACTTTGCAGCTCGGGGGGGCCACACACCTATTCTCGACCG
  GCTCCTGCTCATGGGTGCCCCCATCATGAGAGACTCTTGGGGAGGGACTCCCCTGCATGATGCAGCCGAG
  AATGGACACATGGAGTGCTGCCAGACTCTGCTCTCCCACCACGTAGACCCCTTCCTGCGGGATGAAGATG
  GGTATACAGCGATAGACCTGGCAGAGTACCATGGACACCAGGATTGTGCCCAGTTCCTGCGGGAAATGTC
  TCGGCCGGTGCGGGTCCTGATGACACCCCCACCCCCACCATTTCCACCTCCTCCACTGCTGGCTGCAAAA
  CTCTCCCTGGAAGAGGAAAGAAGAGGGGACTCGGGACTCAAAAGCCCCTCATCTGCAACTCTCAGCCCAG
  TCTGGCCTGCTCAGCCTGTCCCAAGGGAACCGATGGCCTGCACGGCCCCTCTGAGGGTCACCACCCCAGA
  TGCTCTTCAGGGGCCTGAGGTGGAAGCCCGGGACTCCCGTGCTGGCCTAGCCACTCTGCAGCTGGATGGC
  TTGCCTGCGGGAGATCTTGACATGCTGGTGCCCACACAGGACGAGCGTGGCCGGCCCATCCCTGAGTGGA
  AGCGGCAGGTGATGGTGCGGAAGCTACAGGCTCGCCTGGGTGCAGACCATCCTCCAGAAGACCAGGACCA
  GAGTCAGAGGCAGGACTCAGGGCCCACCGCTGCAGAACAGGCAACTTGGAGATATTCACAGACGCACCAG
  GCCATCCTGGGCCCCTTTGGAGAGCTGCTGACGGAGGATGATCTTGTATACCTGGAGAAGCAGATCAACG
  ACCTGCAGCTCCGGCGACGCTGCCAGGAGTATGAGAGTGAGTTGGGCCGGCTGGCAGCCCAGCTGCAGGC
  CCTCCTGCCCGAGCCCCTGGTCAGCATCACCGTCAACAGCCACTTTCTGCCCCGGGCTCCCGGCCTGGAA
  GACGAGGAAGCTCCAGTCCTGGCGACTGAACTCGAAGCCTCCGAGGAGCCGGGGAAGGCCGAGCCCAGAG
  GGCAGCCGCTGCCCTTCTGGTGCAGCCACATCGGTCGCCTGGTGCGCAGCATGTCCCTGCTGCTGAAGGG
  TATGAACGGGCTGGCACAGGGTGAGGAGAAACCACCCTCACCGGTCCCGCAGGACCTAGGCAAGGAGACC
  ATTGCCGGCCCCTCCCGGAGCGAGGCCCAGCGCGAGATCCAAGAGTGCGGAGTGTCAGTTCGGACATTGC
  GGGGGAACTTCGAGTTTGCCCCTGATCTGCCCTGCGCCTTGAACTCGGGTCCCTGTGAGCTGGGGGTGCG
  GCCTGGCCAGTGTCTGAGAGGTTGCTGGTCGGCGCCGCCACAGCCCCGAGGCGACGCGATGGGTGGGGAG
  CCCGGGCCAGGCGACACAGAGGAGGCCAGCGACTCGGGCATCAGCTGCGAAGAGGCCCCGTCAGAGGCCG
  GCGCGGGTCCAGGGCTGGACCTGGCCAGCTTGCGCAAGGAGCGCATCGTCATGCTCTTCCTCGGCCATTG
  GAAGAAGTCAGCCTACACGCCCGCGCTCCGGACGGCAGCCTGCAGGACCCTGGAGGCTCAGCGTGCACGA
  TCCAGGGGCCCAGAGGCCGCGGGTAGCCCCCGGCCGTCCTCCCCGCAGCCCAGCGATGGCCCTCGGCTTG
  GCCACCTGTGGCAGCAGCGTGGCATCATCACCCACCTGCTGGGCACCTGGAAAGCCATCATGGCGCACGT
  GCCCGCCCGGCAGCTGCGGAGGCTGAGCCGCAGGGAGCGTGGGCCCCTGTCTCCGGAGCAGTTCTTGCCG
  CACGTGGACGGGGCCCCCGTGCCCTACAACAGCCTCTCGCTGGACCTCTTCATGCTTGGCTACTTCCAGC
  TCCTGGAGTGCGACCTGCCAGCCGAGGAGCGCAAGATGCGCCACCTGCTGTGCTTCGAGGTTTTCGAGCA
  TCTGGGCGCACACGGCTGGGAGGCTGTGCGAGCCTTCCATAAGGCAGTGACCGACGAGGTGGCCGCGGGT
  CGCCGCGCCTGGACCGACGGCTTTGAGGACATCAAAGCCCGCTTCTTCGGCTCCAGCCAGGGTCCCCCCT
  GGGACGTGGAGCCAGGTCGCAAGCTGGGCCTGACACCACTCGGGTCCTTGCCCCATGCTTCCCTTCCTGG
  CAGCGGCCCTGAACCTGCGGTGCCGCCGAGGCTGGGGTCTGATTCCCAGGGGAGCAGCTTCAACAGCGGA
  GACATCTGTGGCTACATCGACCGCAGCTTCGCATTCTGGAAGGAGAAAGAGGCTGAGATGTTTAACTTTG
  GAGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033292
• Insert Size: 3018 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033292.3, NP_001028464.1
• RefSeq Size: 5442 bp
• RefSeq ORF: 3018 bp
• Locus ID: 227357
• UniProt ID: Q3UYR4
• Cytogenetics: 1 D
• Gene Summary: Binds to but does not cross-link actin. Required for the formation and maintenance of inner ear hair cell stereocilia and staircase formation. Essential for normal hearing.[UniProtKB/Swiss-Prot Function]