Evc (NM_021292) Mouse Untagged Clone

CAT#: MC223178

Evc (untagged) - Mouse Ellis van Creveld gene homolog (human) (Evc), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Evc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223178 representing NM_021292
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTGCACTAAAGATGCGCGGCTGCAGCTGGGCCGGGAAGCCCTGCAGGCGGCGCCGACGCTGCTGG
  TCCCCGCGGTGCTACTGGGTGGCGTTCTCGGCCTAGGCCTTGGCTTGTGGCTTGGCTGTCGCGCGAGCCA
  CTTGCGCGCACGGCTCCAGAAAGATGACAGGAAACGTCTGCTCGGGAGTTCAGAGCCCCCTGCACAGAGC
  CTACGGGACACTGGCTCTCAAGCCAAAGCCAGGAGGCGCCAGAGAGAAACCACTCGGGATGAAGACGCCC
  CTGAGGTGTGTGAGCCATCCTTGAGTGGCAACATCACGGCATTTGCACTGAAAGCCAGAGTTGTCTATCC
  CATCAACCAGAAGTTCCGGCCTCTGGCCGACGGATCCTCCCACCCATCGCTGCATGAAAACCTGACACAG
  GCAGCGGCCATCCTGCCCCACCTGCCCCACCAGCCAGCAGAGGCCTCACCAGCCAGCAGCCTGGGCAGCC
  TGAGCCAGGCTGGCAAGGAGGACGGCAGCTCCTCCTCCAGCATGCGGTCAACCTACAGCGACGACCGGAT
  CCTCCAGTGTGCCTTTCTCCGGGTGGGCAGCTTCCCTGAGATCCTGGCCTGTGAGAGTGTGGACATTGAC
  CTGTGTGTCTGCAGCCTTCACCTGAAAGACCTGCTTCAGGTAGACACGGCGCTGAGGCAAGAGAAGCACC
  TGATGTTCATTCAGATTCTTAAAGCATGCCTCCTGGATTTTTTTCCTAAAAAGAAGCCTGATGATGAATT
  ATGCCAGAAGGTTCTTTCCAAACAAGAGCATGATTTGGAGGAATTAGAAAAGGGACTCCAGGCCAGATTG
  GCGAACACAGAAATGTTAGGCACTGGCGACTCTGGCTACGTTTCCCTGGCAGATGTGGAGAGGAAGGAGA
  GAGAGCTCTCAGAGCAGCTCATAGACAACATGGGAGCTTTCTGGAAGCAGATGGAAAGCATCCAGCCCAC
  TCTCATGGACCAGTTTAAGTGTTCCAGCTCCAAAGCTCGGCAGTTCATGATGACTCTGACAGGAAGAATG
  ATTGTCGCTGAAGGACTGCTGCATGACTCTCAGGACCTGCATGTCCTGGACACCCTGGAGAGGACAATGG
  GTCGATCCCACTTGGCAAGAATGGTGGAGTTCCTGAGAACCCAGATCCAGGAGGAGACCAAGTGCAGACT
  TGCTGCCATCTCCCGTGGCCTGGAGTTGCTGACTGTCCAGGGGCAGCTGTCTGGGAGGCAAAAGGAGGAA
  CTCCTGACTCAGCAGCACAAGGCCTTCTGGGAGGAGGCAGAGCGTTTTGGCAGGGAATTCACGCAGCGGG
  GCAAAGATCTGGTCCAGGCGTCTCAGGCTCGCCAGGCAGAGGCAGCAGCAGAGCTCACACAGACCCAAGA
  AGAAGAACGGAGGAGTTTCCTGGCTGATTCCCAGCTGACCTCGGATCCTGGGGAATTCCTCAAGGCTTTT
  CATGAGGTCCTGGAGAGGCAGCGGCTGACAAGGAGTGACCAAGAGGGAGACGAGGACACCCGAATCACTG
  AGGCCATGGCTGCGCTCTGCCAGGAGCTGTACTGTAGCACCATGGGAACCTTCCAAAAGTTTGTGGACTC
  CCTGTTCCTGAAGACCCTCCCAGAAGTGACCAGCCTCCCTGTGGCAGAATGTGAGACCCTGCGGCAGCAG
  GTCCAGGAGCAGGCAGCAAGGCAGCTGGGACAGGCAGACCGATTCCGCAGACGACAGTGGGGACTCCTAT
  GTGATCTATTGGAGCAGGACAAACGGGTGTGGCTGGAGGAGGGTACGCTGTCCACGGTGCTGCAGAGGCA
  GCTGCGGGATCACCACGAGAGCACCATCCACGGCGTCCTGAGCAGATTCAGTGGCCTTTCTGAAGAGTCC
  TCACGAGGCATCCTGCAGGGCCACGAACTGCTGCTGTGCTCTGCTCTGCGCAGACTGGCCCTCCGAGGCA
  CCACCATCACTGCCCTGGCTCAGATGAGGCTGTCTGGGAAGAAGAGGCTCCTGCAGGAGCTACATGAGCA
  GCTGGCATTAGAGCAGGGTGTGTCCCCATGCCTGGAGGAGCATCAATGGCAGCTGCTTAGAGCCCTGGAG
  GCGCGGATCCAGGAGGAGGCAGCCAGGTTGGAGGATGAGGCACAGCAGACAGGTCTGCGGCTCCAGCAGC
  AGCTGCTGGCGGAGGCACAGGAGGCAGGGCGGCTGCTACAGCTGCACATGGAGCGGGTCATTGGGCAGGC
  GCTGCTGGTACATGCTAGGAATGTTGCCAGCAAAGGCCGGACCAGGGAGAAGGAAGACTTCAAGAGGACG
  CTGGTAGAGACGGTGGTGGAGAGTGTGTATGTGACCAGCACTAGTGTCAACCGCCTCGTGCAGGCGCATT
  ACCAGGCCGTCGGGAAGCTCCTGCAGGCTCATGAGGAACAATTGCTGCAGCGCCTAAAGACCCTGCAGGG
  AGAGAGAATCAATGCTTACAAGCTGTGGAAGAAGCAAGAATTCAGTGACCCGTCGTTGGAGAGTCAAACA
  GCGGATGGTACCCATGGAGCTTCCCAGGGGGTACAGCAGAGGATGCTTTCGCAGCAGAAGAGGCTCCTGG
  ACCAGTTCACGAAGCATCAGCAAGGCCGCCTGAACTCTCAGAGGCAGAAGGCACAAGAGCTGGACCAGCT
  GCAAGCCCAGCTTGAGACCCAGCTGCAGGAAGCTGAGCAGACCCTTATCTCAGAGCTATCGACATTGGCC
  CGAGTACCCCTTCCTGAGAACAAGCCATTCTCCAACAAGCGAGGACTTCCAGAAAAGCCAGTAAGAACCA
  AAAGGAAGAAACCCCCACCTCGGGAGAGAGAGGACCTGGGGACACCAAACGATGACCACCTTGCCTTGGC
  CGACCACACCACAGGACCACTCAGTACAACCTACTCAGCATCGCCTCCCATCCGTGTCCACAGCGGCGGA
  AGGCTGGATCAGCAGGACAGCGAAGCTGGCGATGGAGAAAGCACAAGCAAGATACTGCAGAAAGGAAGCA
  ATCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021292
• Insert Size: 3018 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021292.2, NP_067267.2
• RefSeq Size: 4317 bp
• RefSeq ORF: 3018 bp
• Locus ID: 59056
• UniProt ID: P57680
• Cytogenetics: 5 B3
• Gene Summary: Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling (PubMed:17660199, PubMed:24582806). Involved in endochondral growth and skeletal development (PubMed:17660199).[UniProtKB/Swiss-Prot Function]