Epha8 (NM_007939) Mouse Untagged Clone

CAT#: MC223170

Epha8 (untagged) - Mouse Eph receptor A8 (Epha8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Epha8
• Synonyms: AW047546; Eek; Hek3; mKIAA1459
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223170 representing NM_007939
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCGCCCGGGCCCGCCTGTCCCCCGCTCTCTGGGTCGTCACGGCCGCGGCGGCGGCCACCTGCG
  TGTCCGCGGGGCGCGGCGAAGTGAACTTGTTGGATACATCAACCATCCACGGAGACTGGGGCTGGCTCAC
  GTATCCCGCTCATGGGTGGGACTCCATCAACGAGGTAGACGAGTCCTTCCGGCCCATCCACACGTACCAA
  GTATGCAACGTCATGAGCCCCAACCAGAACAACTGGCTGCGCACGAACTGGGTGCCCCGCGACGGCGCCA
  GGCGTGTCTACGCCGAGATCAAATTCACCCTGCGTGACTGCAATAGCATCCCAGGGGTGTTAGGTACCTG
  CAAGGAGACCTTCAACCTTCACTACCTGGAGTCTGACCGCGACCTGGGGGCCAGCACACAAGAGAGCCAG
  TTCCTCAAGATTGACACCATCGCGGCGGATGAGAGCTTCACGGGCGCAGACCTGGGTGTGCGGAGGCTCA
  AGCTGAACACGGAGGTGCGTGGCGTGGGCCCGCTCAGCAAGCGCGGCTTCTACCTGGCCTTCCAGGACAT
  AGGTGCCTGCCTCGCCATCCTCTCACTCCGAATCTACTACAAGAAGTGCCCCGCCATGGTACGCAACCTG
  GCAGCCTTCTCGGAGGCGGTTACTGGGGCCGACTCGTCCTCGCTGGTTGAGGTTCGAGGCCAGTGTGTGC
  GGCACTCGGAAGAGCGCGACACACCCAAGATGTACTGCAGTGCTGAGGGCGAATGGCTGGTGCCCATCGG
  CAAGTGCGTGTGCAGTGCTGGCTATGAGGAGCGGCGGGATGCCTGCATGGCCTGTGAGCTGGGCTTCTAC
  AAGTCAGCCCCCGGGGACCAGCTGTGCGCCCGATGCCCACCCCACAGCCACTCTGCCACCCCTGCGGCCC
  AGACCTGCCGTTGTGACCTCAGCTACTACCGCGCGGCCTTGGACCCACCATCGGCAGCCTGCACCCGGCC
  ACCCTCAGCACCAGTGAACCTGATCTCCAGCGTAAATGGAACGTCTGTGACCCTCGAGTGGGCCCCTCCT
  CTGGACCCAGGTGGTCGCAGCGACATCACCTACAACGCAGTGTGCCGCCGCTGCCCCTGGGCCCTGAGCC
  ACTGTGAGGCTTGTGGGAGTGGAACTCGCTTCGTCCCGCAGCAGACAAGCCTGGCACAGGCCAGCCTGCT
  GGTGGCCAACCTGCTAGCCCACATGAACTACTCCTTCTGGATCGAGGCAGTCAACGGTGTCTCCAACCTG
  AGCCCTGAGCCCCGCAGCGCTGCTGTGGTCAACATCACCACGAACCAGGCAGCCCCGTCCCAGGTGGTGG
  TGATCCGGCAGGAGCGGGCTGGGCAGACCAGCGTGTCACTCCTGTGGCAGGAACCAGAGCAGCCCAACGG
  CATCATTCTGGAGTACGAGATCAAGTACTACGAGAAGGATAAGGAGATGCAAAGTTACTCTACCCTCAAG
  GCTGTCACCACCCGCGCCACCGTGTCAGGCCTCAAACCAGGCACTCGCTACGTGTTCCAGGTCCGAGCCC
  GCACCTCAGCAGGCTGTGGCCGCTTCAGCCAGGCCATGGAGGTGGAGACCGGGAAACCCAGGCCCCGCTA
  CGACACTCGGACCATCGTCTGGATCTGCCTGACACTCATCACGGGCCTGGTGGTGCTCCTCTTGCTTCTC
  ATCTGCAAGAAGAGGCACTGTGGCTACAGCAAGGCGTTCCAGGACTCGGATGAAGAGAAGATGCATTACC
  AGAACGGGCAGGCACCCCCACCTGTCTTCTTGCCCTTGAACCACCCCCCGGGGAAGTTCCCAGAAACCCA
  GTTCTCTGCAGAGCCCCACACCTATGAGGAACCAGGTCGGGCAGGCCGGAGCTTTACCCGAGAGATCGAG
  GCATCCAGGATCCACATTGAGAAGATCATAGGCTCGGGGGAGTCAGGAGAAGTGTGCTATGGGAGGCTGC
  AGGTGCCAGGACAGCGCGATGTGCCTGTGGCCATCAAGGCTCTCAAAGCTGGCTACACGGAGAGACAGCG
  ACAAGACTTCTTGAGCGAGGCAGCCATCATGGGCCAATTTGATCACCCTAACATCATCCGCCTTGAGGGT
  GTGGTCACCCGTGGTCGCCTGGCAATGATTGTGACGGAGTACATGGAAAATGGCTCTCTGGATGCCTTCC
  TCAGGACCCACGATGGGCAGTTCACCATCGTGCAGCTAGTGGGCATGCTCAGAGGGGTGGGCGCTGGCAT
  GCGCTACCTCTCAGACCTGGGCTACATTCACCGTGACCTGGCTGCCCGCAATGTCCTGGTGGATGGCCGT
  CTGGTGTGCAAAGTGTCAGACTTTGGGCTCTCAAGGGCTCTGGAGGATGACCCCGAAGCTGCCTACACCA
  CTGCGGGAGGGAAGATCCCTATCCGATGGACAGCGCCGGAGGCCATTGCTTTCCGCACGTTCTCCTCAGC
  CAGTGATGTGTGGAGCTTTGGTGTGGTCATGTGGGAGGTTCTAGCCTATGGTGAACGACCCTACTGGAAC
  ATGACCAACCAGGATGTCATCAGCTCTGTGGAGGAGGGTTATAGGCTGCCAGCACCCATGGGTTGCCCAC
  GTGCCCTACACCAGCTCATGCTGGACTGCTGGCACAAGGACCGTGCCCAGCGGCCTCGCTTCGCTCATGT
  CGTGAGCGTTCTGGATGCCCTGGTCCACAGTCCTGAGAGCCTCAGGGCCACAGCCACAGTCAGCAGGTGC
  CCACCCCCTGCCTTCGCCAGGAGCTGCTTTGACCTCCGAGCAGGCGGAAGTGGGAACGGGGACCTCACAG
  TGGGGGACTGGCTGGACTCCATCCGAATGGGGCGCTACCGGGACCACTTCGCGGCTGGCGGGTACTCCTC
  CCTCGGCATGGTGCTTCGGATGAACGCCCAGGATGTGCGCGCCCTCGGCATCACTCTCATGGGTCACCAG
  AAGAAGATACTTGGAAGCATCCAGACCATGAGGGCCCAGCTGAGCAGCACCCAGGGACCCCGCCGGCACC
  TCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007939
• Insert Size: 3015 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007939.2, NP_031965.2
• RefSeq Size: 4732 bp
• RefSeq ORF: 3015 bp
• Locus ID: 13842
• UniProt ID: O09127
• Cytogenetics: 4 D3
• Gene Summary: Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The GPI-anchored ephrin-A EFNA2, EFNA3, and EFNA5 are able to activate EPHA8 through phosphorylation. With EFNA5 may regulate integrin-mediated cell adhesion and migration on fibronectin substrate but also neurite outgrowth. During development of the nervous system plays also a role in axon guidance. Downstream effectors of the EPHA8 signaling pathway include FYN which promotes cell adhesion upon activation by EPHA8 and the MAP kinases in the stimulation of neurite outgrowth.[UniProtKB/Swiss-Prot Function]