Ccp110 (NM_182995) Mouse Untagged Clone

CAT#: MC223165

Ccp110 (untagged) - Mouse RIKEN cDNA 6330503K22 gene (6330503K22Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccp110
• Synonyms: 6330503K22Rik; AA415922; AI427129; AW557948; CP110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223165 representing NM_182995
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAATATGAGGAGTTCTGCGAGAAAGCACTTGGCCGAGCTCAGGAAGCATCGCTGTCCACAGGCA
  GCTTCCTGCCCGCTCAGGCAGAAAGTGTCTCACTCATCCGTTTCCATGGGGTGGCTGTACTTTCTCCACT
  GCTTACCATTGAGAAGAGAAAGAAAATTCAAGAGGAAAAGCAGAAAGCACTTGATGTGCAGTCAAGAAAG
  CAGGCCAACAGGAAGAAAGCCTTACTGACTCGTGTCCAGGAGATTCTTGAAAATGTTCAGGTTAGAAAAG
  CACCAAATGCCAGTGATTTTGATCAGTGGGCAACAGAAACCATTTACTCTAATCCAGAAGTCACAGACTT
  GAATGTTCCTGTTAGAGTTCCAAACAGCTTGCCGAGCCCCACCGAACACTGTACTTCAGTAAAGCTTGAA
  AAGATAACTGGACTTTTGCCTGTGAATAATGAGGACCAACAAACCCCTAAGCGGGTGGGGCTGCCTGGAG
  ACTCAGAGGTGTCTGGTTCTCTGAGGCAGTGTGAGAGTCCAGAAAGCAGGCAGGCAGAAGATGGAGCTGC
  TCTAAGGCTTTCTTCAGCAAGCCCACAGGAGACCATCATTTCTGATGTCTTAGGAAAGGAAGAACAGGAC
  CCATCATGTTTGGCTGAAGTCACTCCAGATCCCTACATAATGAGCCTTCAGAATCTAATGAAGAGGTCAA
  AGGAATACGTGGAAAGAGAACTGTCTAGCCGGAGTCTGAGAAATAGTTTAAAGAGAAGTGTTAATGAAAC
  GCATTCAGACAGAGAAAACGATGCTGCTAAGGCGAGCGACTGTGTAAAGGAGAAGGCCCCACCCATGCCC
  ATAGGCAGGCACTGTGGCTCTGCCATCCCTGACAAACCAAGCCTTAATAAGTCAAATGTTCTCCTCCAAG
  GTGCTTCACAAGCAAGCAGCATGGGCACGGCAGGCTTAGCTAGCTTTTCTAAGATAGACCTGCCTGCAGG
  AGCTGCTCCTCCCGCTGCTCCAGATGCCGGATCAGATTTTACAGTTATTCCTACCTTTGTTACTGAAAAT
  AAAGTTAAAAGTCTTAAAGGTCCATATGCCAAATTACCTAGTCCAGAGCCAAGTATGAGTCCTACGATGC
  ATCGGAGGCATTCTCGGTCAGCATCAGCATGTCAGATCCTTATAAACAACCCAGTAAATGCCTGTGAGCT
  GAGCCCAAAGGGGAAAGAAGAGGCAGTGGATAGAACCGCTCCAGCTGCTGCTGAGACAACAAATGAGTCT
  GAAACTGTGCCAAAATCACCGACTGATTTGACAGGGGTTTGTTCAAGTAATGTTTCTGCTACCAAAATCA
  CGTCTGAGAGCACAAGAGAGATGGTCGTGGGTAAGCCAAGTCAGAGGCAGCAGGCTTTAGGAGCTCACTT
  AGGCAATAACGTCACTGTTGAGCGGTCTGCTATGGAAGGTCCCTTCATAGCTGATGACAGGGGAGCCCAG
  AAGGTGGACGGTACTTGTATGGCTGTGCCCAAGTTGCATGAACTGCAGCCCAGCAGTCAGTGTGTGTCAA
  GTCAAACCTTGGAAGATGTGTGTGAACTCAAGTCAGCCAGTCTGCTAGCAAAGAACTCCTGCAACTTACA
  AATGGAACTGAATAAATCTTACGATGTGAAACACCCATCTCCCTTACTTACGCAAACCCAGACTTCCAGA
  CAGCAGATGGACACACCGCCGGTGTTCCGTGGAAACGAGCAGTTTGTAGATAACAGTTTTGAGAAAGTTA
  AGCGGAGACTTGATTTAGATGTTGATAGTTTACAAAAAGAAAATTGTCCTTATATTATAACAGCTGGAGT
  AGCTGAGCAGGAGCGGGACCGTCTGCTGGAAAGAAGATACCCCAAGGGATTCGTCCACATTAACAAGAAT
  AAGATGCTAGAAACTAGCCCCAAAGAAGGCCAGGAACTACTGAAAAGCAAGATGTTAGCTTTTGAAGAAA
  TGCGAAAGAGACTAGAGGAACAGCATGCCCAGCAGTTGTCACTCCTCATAGCTGAGCAGGAGAGGGAGCA
  GGAGCAACTGCAAAAGGAAATAGAAGAGCAGGAGAAGATGCTGAAGGAGAAGGCAGTTACGACGGACGTC
  TCTGACTTGAACAGTGCACTGGAGTGGAGACAAAGAACCGATTCTGCTTTGCTAGAAACCATGCTGTCCC
  AAGTGGACTCACTCCAGACTTCAAACAACTCTGGCTTCATAACTTCTGCCCTGCAGTACAGCTTCGGCTC
  TGCAGGTGAAGCACCGTTCTACCTCTGGGGATCACTGACCAGTGGCGTGACCAGAGTCTCAGGAACAAGG
  CCTTGTGGACGGGCCCAAGCTAAATGGTCTCAGGTTTTTAATCCAGAAATACACGCAAAATTTAATAAAA
  TAACTGCCGTGGCAAAAGGGTTTCTTACTCGTAAACTTATGCAAACAGACAAACTGAAGCAGCTTCGACA
  AACTGTAAAAGACACCATGGAATTCATACGAAGTTTTCAGTCAGAAGCCCCTCTGAAGAGAGGTGTCGTC
  TCAGCCCAAGATGCTTCCCTTCAGGAACGAGTGTTAGCTCAGTTGCGAGCTGCTCTGTATGGTATTCATG
  ACATCTTCTTCGTAATGGATGCAGCCGAGAGAATGTCAATCCTCCATCATGATCGAGAAGCCCGCAAAGA
  GAAACTGCTCCGGCAAATGGATAAGATGAAAAGTCCACGAGTGGCGCTTTCAGTGGCAACACAGAAGTCC
  CTGGACAGGAAGAAGTTCATGAAAGTTGCTGAGATGGGAATGCCAAATAAGAAGTTCCTGCTTAAACAAA
  ACCCCTCAGAAACAAGAGTCCTTCAGCCAAACCAAGGACAGAATGCACCCGTTCATAGGCTACTTAGTAG
  ACAAGGAACCCCTAAGACTTCAGTGAAGGGGGTTGTGCAAAATAGACAGAAGCCTTCACAGAGCAGAGTG
  CCTAACAGAGCGCCTGTGTCAGGAGCATATGCAGGAAAAACCCAAAGAAAGCGGCCAAATGTTGCGACAA
  TTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_182995
• Insert Size: 3015 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182995.1, NP_892040.1
• RefSeq Size: 5024 bp
• RefSeq ORF: 3015 bp
• Locus ID: 101565
• UniProt ID: Q7TSH4
• Cytogenetics: 7 F2
• Gene Summary: Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation (PubMed:23141541). Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation (PubMed:26965371). Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2 (By similarity).[UniProtKB/Swiss-Prot Function]