Nlrp9b (NM_194058) Mouse Untagged Clone

CAT#: MC223160

Nlrp9b (untagged) - Mouse NLR family, pyrin domain containing 9B (Nlrp9b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nlrp9b
• Synonyms: Nalp-delta; Nalp9b; Nlrp9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223160 representing NM_194058
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCTCATCTGGCTATGGCTTGCTGAAGCTTCTGCAAAAACTGAGTGATGAGGAGTTCCAGAGAT
  TTAAAGAGCTTCTCAGAGAAGAGCCTGAAAAGTTTAAACTCAAACCAATATCCTGGACGAAAATCGAGAA
  TTCTTCCAAAGAAAGCCTGGTAACGCTATTGAACACACATTATCCAGGACAGGCATGGAATATGATGTTA
  AGCCTGTTTCTACAGGTCAATCGGGAAGACCTCTCAATCATGGCTCAGAAAAAGAAGAGACATAAACAAA
  CTAAATACAAAAAGTTTATGAAGACTACATTTGAAAGGATATGGACATTGGAGACCAACACTCATATACC
  TGATAGAAACTACCATTTAATTGTAGAAGTCCAGTACAAAGCATTGCAGGAAATATTTGATTCTGAGTCA
  GAGCCAGTCACTGCAATTGTAGCAGGTACAACAGGAGAAGGAAAAACCACTTTCTTAAGAAAAGCAATGT
  TGGATTGGGCATCAGGAGTTTTACTGCAGAACCGGTTTCAGTATGTTTTCTTCTTCTCTGTCTTCTCACT
  TAACAATACCACAGAGTTAAGCTTAGCCGAGCTTATCTCAAGTACGTTGCCTGAATCTTCAGAGACAGTG
  GATGATATTTTATCTGATCCAAAAAGAATCTTGTTTATCCTGGATGGATTTGATTACCTGAAATTTGACT
  TGGAACTCAGGACAAACTTGTGCAATGACTGGAGGAAGAGGCTGCCAACACAAATCGTCTTGAGTAGTTT
  GCTACAGAAAATAATGCTCCCGGGATGTTCTCTGCTTCTTGAATTGGGACAAATAAGTGTACCAAAAATC
  CGTCATTTGCTGAAGTATCCAAGGGTGATAACTATGCAAGGATTCTCTGAGCGGTCTGTAGAGTTCTATT
  GTATGTCCTTCTTTGATAACCAGAGAGGCATAGAAGTAGCTGAGAATTTAAGGAATAATGAAGTACTCCA
  TTTATGCAGCAATCCTTATCTATGCTGGATGTTCTGTAGTTGTTTAAAGTGGCAGTTTGACAGGGAGGAG
  GAAGGGTACTTTAAGGCTAAAACAGATGCGGCTTTCTTCACAAACTTTATGGTGAGTGCATTCAAATCAA
  CATATGCCCATAGTCCGTCCAAACAGAACAGAGCCCGATTAAAGACCCTGTGTACCTTGGCTGTAGAAGG
  AATGTGGAAAGAGTTGTTTGTCTTTGACTCTGAAGATCTCAGGAGGAATGGGATATCTGAATCCGATAAG
  GCAGTGTGGCTGAAAATGCAATTTCTCCAGACTCACGGTAACCACACTGTGTTTTATCATCCTACACTAC
  AGTCATATTTTGCTGCCATGTTCTATTTCCTCAAACAAGACAAAGACATATGTGTCCCAGTCATTGGAAG
  CATACCTCAACTTCTAGGAAACATGTATGCTCGTGGCCAGACCCAGTGGCTCCAGTTAGGCACATTTTTG
  TTTGGACTCATCAATGAACAAGTCGCCGCCTTACTCCAACCATGCTTTGGCTTTATACAACCCATATATG
  TAAGACAGGAAATCATTTGTTACTTTAAGTGTCTTGGTCAACAAGAGTGCAATGAGAAACTGGAAAGATC
  CCAGACTTTGTTCTCCTGTCTGCGTGACAGCCAGGAGGAAAGATTTGTTAGACAAGTGGTGGATCTACTT
  GAAGAAATTACTGTTGATATTAGTAGTTCAGATGTATTATCAGTGACTGCATATGCTCTGCAGAAATCCT
  CCAAATTAAAGAAACTTCATCTGCATATACAAAAGACAGTTTTTTCAGAAATCTACTGCCCAGACCATTG
  TAAAACAAGAACTTCTATAGGCAAAAGACGAAATACAGCCGAATATTGGAAAACATTATGTGGCATTTTC
  TGCAACTTATATGTGTTGGATCTGGACAGCTGTCAGTTCAATAAAAGGGCCATACAGGACCTCTGTAATT
  CGATGTCTCCAACTCCTACGGTGCCCCTGACTGCTTTTAAGCTCCAAAGCTTGTCGTGTTCCTTCATGGC
  TGACTTTGGAGATGGTTCATTATTTCACACCCTTCTTCAGCTACCTCACCTGAAATACCTGAACCTGTAT
  GGCACTTATCTCTCCATGGATGTGACTGAGAAGCTGTGTGCTGCTCTGAGATGCTCAGCATGCAGAGTGG
  AGGAGCTGCTGTTGGGTAAATGTGGCATCTCAAGTAAGGCCTGTGGCATAATTGCGATTTCCCTTATCAA
  CTCTAAAGTGAAACACCTCTCATTGGTTGAAAATCCCTTGAAGAACAAAGGAGTGATGTCACTGTGTGAA
  ATGCTGAAGGATCCAAGCTGTGTCCTGCAGTCACTGATGTTGTCTTACTGCTGCCTCACATTCATTGCCT
  GTGGACATCTCTATGAAGCACTTTTGAGCAACAAACACCTCTCCCTGCTTGATCTGGGATCAAATTTCCT
  GGAAGATACTGGAGTGAACCTTCTGTGTGAAGCTTTGAAAGATCCAAACTGCACCCTCAAGGAGTTATGG
  TTGCCGGGTTGCTTTTTAACTTCACAATGCTGTGAGGAGATCTCGGCTGTCCTCATTTGCAATAGAAATC
  TAAAGACCCTGAAACTGGGCAACAATAACATACAAGACACTGGTGTCAGACAGTTATGTGAAGCTCTGAG
  TCATCCCAACTGTAACTTGGAGTGTCTTGGGTTAGACCTGTGTGAGTTCACCAGTGACTGTTGTAAGGAC
  CTAGCCTTGGCTCTCACCACCTGCAAAACACTGAATAGCTTAAATCTGGACTGGAAAACCTTGGACCACT
  CTGGGCTGGTGGTGCTCTGTGAGGCTTTGAATCACAAAAGATGTAACCTGAAGATGCTTGGACTGGACAA
  ATCTGCGTTTTCCGAGGAATCACAGACATTACTGCAAGATGTGGAAAAGAAAAATAATAATCTGAATATT
  TTGCATCATCCCTGGTTTGAGGCGGAACGCAACAAGAGGGGTACTCGTCTGGTATGGAACAGCAGGAATT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_194058
• Insert Size: 3012 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194058.2, NP_918947.2
• RefSeq Size: 3346 bp
• RefSeq ORF: 3012 bp
• Locus ID: 243874
• UniProt ID: Q66X22
• Cytogenetics: 7 A3
• Gene Summary: As the sensor component of the NLRP9 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens, including rotavirus, initiates the formation of the inflammasome polymeric complex, made of NLRP9, PYCARD and CASP1. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and release in the extracellular milieu. The active cytokines stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. NLRP9 inflammasome activation may be initiated by DHX9 interaction with viral double-stranded RNA (dsRNA), preferentially to short dsRNA segments.[UniProtKB/Swiss-Prot Function]