Ptprn2 (NM_011215) Mouse Untagged Clone

CAT#: MC223149

Ptprn2 (untagged) - Mouse protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptprn2
• Synonyms: 4930425H11Rik; IA2beta; mKIAA0387; phogrin; Phol; PTP-NP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223149 representing NM_011215
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCGCCGCTCCCGCTTTTGCTGCTGCTGCTGCTACCGCCGCCGCTGCCTCGCGCTCTGCCCGCCC
  CCGCGTCTGCCCGCGGCCGGCAGCTCCCGGGGCGCCTGGGATGCTTGTTTGAGGATGGCTTGTGTGGATC
  ACTGGAGACCTGTGTGAACGATGGTGTGTTTGGAAGATGTCAAAAGGTTCCGGTGATGGACACTTACCGA
  TATGAGGTACCACCAGGAGCCCTGCTGCACCTGAAGGTCACCTTACAGAAGCTCTCCCGTACAGGTTTCA
  CGTGGCAAGATGACTATACCCAGCGTGTGATTGCCCAGGAGCTTGCAAACCTCCCCAAGGCCTACCTATG
  GCATGGGGAAGCGTCCGGCCCAGCCAGGTCCTTACAACAGAATGCTGACAATGAAAAATGGTTCAGTCTG
  GAGAGGGAGGTGGCCCTGGCCAAGACCCTTCGGCGCTATCTGCCCTACCTGGAGCTTCTGTCCCAGACCC
  CAACAGCAAATGCACACTCTAGGATAGACCATGAGACTCGTCCAGCCAAGGGTGAAGACTCTTCCCCTGA
  GAACATCCTGACCTACGTGGCCCACACATCAGCACTGACCTATCCTCCTGCAACCCGGGCCAAGTATCCT
  GATAACCTTCTGCGGCCCTTTAGCCGGCTCCAGCCAGATGAGCTCAGCCCCAAGGTAGACGGTGACATAG
  ACAAACAGAAGCTGATTGCAGCACTGGGCGCCTACACTGCTCAGAGGCTTCCTGGAGAAAATGACCCAGA
  GCCACGGTACCTTGTACATGGTTCCGCGAGAGCACCAAGGCCATTCTCAGCAACTGCTTTGTCTCAGAGA
  TGGCCTCCACCTCCTGGAGACGCCAAAGACTCCCCGAGTATGGATGATGACACACTCCTGCAGAGTCTCC
  TGAAGGATTTGCAGCAGAACTCTGAAGTGGACCGCCTGGGCCCCCTGAAGGAGGAGAAAGCAGACTCAGT
  TGCTGGAGCCATACAAAGTGATCCTGCAGAGGGAAGCCAAGAAAGCCACGGGAGAGGGGCTGAAGGACAG
  CCAAGAGAGCAGACAGATGCCCCAGAGACAATGCTTCAAGATCACAGACTATCAGAGGTGGATGACCCAG
  TGTACAAGGAGGTCAACCGTCTGAGCTTCCAGCTTGGGGACCTCTTGAAGGACTATGGGTCTCCTCTCTT
  ACCTGAAGGTCCCCTTCTAGAAAAATCCTCCAGAGAAGAGATTAAGAAATCAGAGCAGCCAGAGGAGGTC
  TTGTCTTCAGAAGAGGAGACTGCTGGGGTGGAGCATGTGAGGAGCCGGACTTACTCCAAAGACCTATTTG
  AAAGGAAACCAAACTCAGAGCCCCAGCCCAGGAGGCTTGAGGATCAGTTCCAAAACCGAGCTCCAGAGTT
  GTGGGAGGATGAAGAAAGCCTCAAATTGGCAGCACAGGGACCCCCTAGTGGAGGCCTACAGCTGGAAGTG
  CAGCCTTCTGAGGAACAGCAGGGATACATCCTCACAGGAAACAACCCTCTAAGTCCAGAGAAGGGGAAGC
  AGCTGATGGACCAAGTTGCCCACATCCTCCGGGTACCTTCCAGCTTCTTTGCAGATATCAAAGTTTTGGG
  ACCAGCAGTGACCTTCAAAGTAAGTGCCAACATCCAAAACATGACAACTGCCGATGTCATCAAGGCTGCA
  GCTGACAACAAAGACCAGCTGGAGAAGGCAACTGGACTGACAATCCTTCAAAGTGGAATCAGGCCGAAGG
  GAAAGCTCAAACTCCTGCCGCATCAGGAAGAGCAAGAGGACTCTACCAAGTTCATTTTGCTCACCTTCCT
  CTCCATTGCCTGCATCCTGGGGGTTCTCCTGGCTTCCAGCCTTGCCTACTGCCTCCGCCACAACTCACAC
  TACAAGCTGAAGGACAAGTTGTCTGGACTAGGCGCTGACCCCAGTGCAGATGCCACTGAAGCCTACCAGG
  AGCTATGCCGCCAGCGTATGGCTATTCGTCCACAGGACCGCTCTGAGGGACCACATACATCACGCATCAA
  CAGCGTCTCATCCCAGTTCAGCGATGGGCCGATGCCTAGTCCTTCGGCTCGGAGCAGCACTTCATCCTGG
  TCTGAGGAGCCTGTCCAGTCCAACATGGACATCTCTACTGGCCACATGATCCTGGCCTACATGGAAGACC
  ATCTGAAGAACAAGAACCGCCTGGAGAAGGAGTGGGAAGCACTGTGCGCCTACCAAGCAGAGCCCAACAG
  CTCACTTGTGGCCCAGAGAGAGGAGAATGCACCCAAGAACCGTTCCCTGGCTGTGCTGACCTATGACCAC
  TCCAGGATCCTGTTGAAGTCTCAAAACAGCCATGGCAGTTCCGACTACATCAATGCCAGCCCCATTATGG
  ACCATGACCCACGAAACCCCGCATACATTGCCACCCAAGGCCCACTTCCCGCCACGGTGGCCGACTTCTG
  GCAGATGGTGTGGGAAAGCGGCTGTGCAGTCATTGTCATGCTGACACCCCTCTCCGAGAACGGCGTCCGG
  CAGTGCCATCACTACTGGCCCGATGAAGGCTCCAACCTCTACCATGTCTACGAGGTCAATCTAGTCTCTG
  AGCACATATGGTGCCAGGATTTCCTGGTGAGAAGCTTTTACCTGAAGAACCTGCAGACCAACGAGACTCG
  CACGGTGACCCAGTTCCACTTCCTGAGTTGGTATGACCAGGGAGTCCCTTCCTCCACGAGGTCACTCCTG
  GATTTCCGCAGAAAAGTGAACAAATGCTACCGAGGCCGCTCTTGTCCGATCATTGTCCATTGCAGTGACG
  GCGCCGGCAGGAGTGGAACCTACGTCCTGATTGACATGGTTCTCAATAAGATGGCCAAAGGTGCTAAAGA
  GATTGATATCGCAGCGACCCTGGAGCACTTGAGGGACCAGAGACCAGGCATGGTCCAGACAAAGGAGCAG
  TTTGAGTTTGCGCTGACAGCTGTGGCTGAGGAGGTGAATGCCATCCTGAAGGCCCTTCCCCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011215
• Insert Size: 3006 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011215.2, NP_035345.2
• RefSeq Size: 4688 bp
• RefSeq ORF: 3006 bp
• Locus ID: 19276
• UniProt ID: P80560
• Cytogenetics: 12 62.65 cM
• Gene Summary: Plays a role in vesicle-mediated secretory processes (PubMed:21732083). Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation (PubMed:21732083). Plays a role in insulin secretion in response to glucose stimuli (PubMed:15220191, PubMed:16418280, PubMed:21732083). Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) (PubMed:16269463). Required to maintain normal levels of renin expression and renin release (PubMed:19019914). May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization (PubMed:12364328). Has phosphatidylinositol phosphatase activity; the PIPase activity is involved in its ability to regulate insulin secretion. Can dephosphorylate phosphatidylinositol 4,5-biphosphate (PI(4,5)P2), phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate (By similarity). Regulates PI(4,5)P2 level in the plasma membrane and localization of cofilin at the plasma membrane and thus is indirectly involved in regulation of actin dynamics related to cell migration and metastasis; upon hydrolyzation of PI(4,5)P2 cofilin is released from the plasma membrane and acts in the cytoplasm in severing F-actin filaments (By similarity).[UniProtKB/Swiss-Prot Function]