Mbtps1 (NM_019709) Mouse Untagged Clone

CAT#: MC223144

Mbtps1 (untagged) - Mouse membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mbtps1
• Synonyms: 0610038M03Rik; AV003995; mKIAA0091; S1P; SKI-1; Ski1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223144 representing NM_019709
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCGTCAGCACCTGGCTTCTTGTGCTGGTGGTTTTGCTCTGTGGGAAACGGCACCTGGGCGACA
  GGCTGGGGACGAGAGCTTTGGAAAAGGCCCCGTGCCCCAGCTGCTCCCACCTGACTTTGAAGGTGGAATT
  CTCTTCAACTGTGGTGGAGTACGAATATATTGTGGCTTTCAACGGATACTTCACAGCCAAAGCTAGAAAC
  TCATTTATTTCAAGTGCTCTGAAAAGCAGTGAAGTGGAAAACTGGAGAATAATACCTCGGAACAACCCAT
  CCAGTGACTACCCTAGTGATTTTGAGGTGATTCAGATAAAAGAGAAGCAGAAGGCGGGGCTGCTCACACT
  TGAAGATCACCCCAACATCAAGCGGGTGACACCCCAGCGGAAAGTCTTTCGTTCCCTCAAGTTTGCTGAA
  TCCAACCCCATCGTGCCCTGTAATGAAACCCGGTGGAGCCAGAAGTGGCAGTCATCACGTCCCCTGAAAA
  GAGCCAGTCTCTCCCTGGGCTCTGGATTCTGGCATGCAACAGGAAGACATTCAAGTCGGCGATTGCTGAG
  AGCCATTCCTCGCCAGGTCGCCCAGACACTGCAGGCAGATGTGCTGTGGCAGATGGGATACACAGGTGCT
  AATGTCAGAGTTGCTGTTTTTGATACTGGGCTCAGTGAGAAGCATCCGCATTTTAAGAATGTGAAGGAGA
  GAACCAACTGGACCAATGAGCGGACCCTGGATGATGGGCTAGGCCATGGCACATTCGTTGCAGGTGTGAT
  TGCCAGCATGAGGGAGTGCCAAGGATTTGCTCCAGATGCAGAGCTGCACATCTTCAGGGTCTTTACCAAC
  AATCAGGTGTCTTACACATCTTGGTTTCTGGATGCCTTCAACTATGCCATCCTAAAGAAGATGGACGTTC
  TCAACCTTAGCATCGGTGGGCCCGACTTCATGGATCATCCGTTTGTTGACAAGGTGTGGGAATTAACAGC
  TAACAATGTAATTATGGTTTCTGCTATTGGCAATGATGGACCTCTCTATGGCACTCTGAATAACCCTGCT
  GATCAGATGGATGTGATTGGAGTGGGTGGCATTGACTTTGAAGATAACATCGCTCGCTTTTCTTCCAGGG
  GAATGACTACCTGGGAATTACCAGGAGGCTATGGTCGTGTGAAGCCTGACATTGTCACCTATGGTGCTGG
  AGTGCGGGGTTCCGGTGTGAAAGGGGGCTGCCGTGCACTCTCAGGGACCAGTGTCGCTTCCCCAGTGGTC
  GCTGGGGCCGTCACCTTGTTAGTAAGCACAGTACAGAAGCGGGAGCTGGTGAATCCTGCCAGTGTGAAGC
  AAGCTTTGATAGCGTCAGCCCGGAGACTTCCTGGGGTCAACATGTTCGAGCAAGGTCATGGCAAGTTGGA
  TCTGCTGCGAGCTTATCAGATCCTCAGCAGCTATAAACCGCAGGCAAGCCTGAGTCCTAGCTACATCGAC
  CTGACTGAGTGTCCCTACATGTGGCCCTACTGCTCCCAGCCTATCTACTATGGAGGAATGCCAACAATCG
  TTAATGTCACCATCCTCAATGGCATGGGCGTCACAGGAAGAATTGTGGATAAGCCTGAGTGGCGACCCTA
  TTTACCACAGAATGGAGACAACATTGAAGTGGCCTTCTCCTACTCCTCAGTGTTGTGGCCCTGGTCAGGT
  TACCTTGCCATCTCCATTTCTGTGACCAAGAAGGCAGCTTCCTGGGAAGGCATCGCTCAGGGCCACATCA
  TGATCACAGTGGCGTCCCCAGCAGAGACAGAGTTACACAGTGGTGCGGAGCACACTTCCACCGTGAAGCT
  GCCCATCAAGGTGAAGATCATTCCCACCCCTCCTCGGAGCAAGAGAGTCCTCTGGGACCAGTACCACAAC
  CTCCGCTACCCACCTGGCTACTTCCCCAGGGACAACTTGCGGATGAAGAATGACCCTTTAGACTGGAATG
  GCGACCACGTCCACACCAACTTCAGGGACATGTACCAGCATCTGCGCAGCATGGGCTACTTCGTGGAGGT
  GCTCGGCGCCCCATTCACATGTTTTGACGCCACACAGTATGGCACTTTGCTGCTGGTGGACAGTGAGGAA
  GAGTACTTCCCTGAGGAGATTGCTAAGCTGAGGAGGGATGTGGACAATGGCCTTTCCCTCGTCATCTTCA
  GTGACTGGTACAACACTTCTGTTATGAGAAAAGTGAAGTTTTATGATGAAAACACCAGGCAGTGGTGGAT
  GCCAGACACCGGAGGAGCGAACATCCCAGCTCTGAATGAGCTGCTGTCTGTGTGGAACATGGGGTTCAGT
  GACGGCCTATATGAAGGGGAGTTTGTCCTGGCAAACCATGACATGTACTATGCGTCGGGGTGCAGCATCG
  CCAAGTTTCCAGAAGATGGCGTCGTGATCACACAGACTTTCAAGGACCAAGGATTGGAGGTCTTAAAACA
  AGAGACAGCAGTTGTGGAAAATGTTCCCATTTTGGGGCTTTATCAGATTCCATCTGAAGGTGGAGGCCGG
  ATCGTGCTGTATGGAGACTCCAACTGCTTGGATGACAGTCACAGACAGAAGGACTGCTTTTGGCTTCTGG
  ATGCGCTCCTTCAGTACACATCCTATGGCGTGACCCCTCCCAGCCTCAGCCATTCAGGGAACCGGCAGCG
  CCCACCTAGCGGAGCCGGCTTGGCCCCTCCTGAAAGGATGGAAGGAAACCACCTCCATCGGTACTCCAAA
  GTTCTTGAAGCCCACTTGGGAGACCCGAAACCTCGGCCCCTGCCAGCCTGTCCACATTTGTCATGGGCCA
  AGCCACAGCCTTTGAATGAGACGGCACCCAGTAATCTTTGGAAACATCAGAAGCTGCTCTCCATTGACCT
  GGACAAAGTAGTGTTACCCAACTTTCGATCCAATCGCCCTCAAGTGAGACCTTTGTCCCCTGGAGAGAGT
  GGTGCCTGGGACATTCCTGGAGGGATCATGCCTGGCCGCTACAACCAGGAGGTGGGACAGACCATCCCCG
  TCTTCGCCTTCCTCGGAGCCATGGTGGCCCTGGCCTTCTTTGTGGTACAGATCAGCAAGGCCAAGAGCCG
  GCCGAAGCGGAGGAGGCCCAGGGCAAAGCGTCCACAACTTGCACAGCAGGCCCACCCTGCAAGGACCCCA
  TCAGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019709
• Insert Size: 3159 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019709.4, NP_062683.3
• RefSeq Size: 4393 bp
• RefSeq ORF: 3159 bp
• Locus ID: 56453
• UniProt ID: Q9WTZ2
• Cytogenetics: 8 E1
• Gene Summary: Serine protease that catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs). Other known substrates are BDNF, GNPTAB and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg-Ser-Val-Leu (SERBP-2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.