Zfp516 (NM_001177464) Mouse Untagged Clone

CAT#: MC223142

Zfp516 (untagged) - Mouse zinc finger protein 516 (Zfp516), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp516
• Synonyms: C330029B10Rik; D230016L03; mKIAA0222; Zfp26l; Znf516
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223142 representing NM_001177464
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGCAGCAGAGAGGCTGAGATGGAGCTGAGGAGAGGACCCAGCCCACCCAGGGCTGGCAGGAGCC
  ACGAAGTGGATGGGGACAAGGCTGCCTGCCACAGCTGCTGCATCTGTGGCAAGAGCTTTCCTTTCCAGAG
  CTCCCTGTCACAGCACATGCGCAAGCACACGGGAGAGAAGCCCTACAAGTGTCCCTACTGTGATCACAGG
  GCTTCCCAGAAGGGCAACCTCAAGATTCATATCCGCAGCCACCGGACAGGAACTCTGATTCAGGGGCACG
  AGCCAGAGGCGGGAGAGGCTCAGCTGGGTGAGATGCGTGTCTCTGAGGGCCTGGATGGGTGTGCCAGCCC
  TACTAAGAGCACCTCTGCCTGTAACCGCGTGCTGAACGGGGCCGTGCCGATGGACGGCAGCAAGATTCTG
  CTCAGGAGCAGCCGCAAGGAGGTGGAGGGTGCGGCCAGCGCCCAGGAAGACACTGAGGCCACGGTGCCGT
  GCTCCTTCTGTAAGAGCCGGTTCGAGCGCAAGAAGGACCTGGAGCTGCATGTGCACCAGGCCCACAAGCC
  GTTCAAGTGCAGGCTGTGCAGCTATGTCACCCTGCGGGAGGAGTCTCTGCTCAGCCACATAGAGAGGGAC
  CACATCACCGCACAGGTGCCCAATGGCAGCGAGGCCTGTGTGGAGAACGGCAAACCTGAGCTGAGCCCTG
  GGGAGTTTCCCTGCGAGGTGTGTGGCCAGGCCTTCAGCCAGACCTGGTTCCTGAAGGCACACATGAAGAA
  ACACCGGGGCTCCTTTGACCACGGCTGCCACATCTGCGGCCGGAGATTCAAAGAGCCTTGGTTCCTCAAG
  AACCATATGAAGGCGCATGGACCCAAGGCAGGAAGCAAGAACAGACCCAAGAGTGAGCTGGATCCCATTG
  CCACCATCAACAATGTGGTGCAGGAGGAAGTCATTGTCGCTGGCCTGTCCCTCTATGAGGTCTGCACAAA
  GTGCGGGAACCTGTTTACAAACCTGGACAGCTTGAACGCCCATAACGCCATACACCGTAAAGTTGAAGCC
  AGCCGGATTCGAGCCCCGGCTGAGGAGGGGGACTCAGAGGACCCCCTGGACACTAAGCAGTTCTTCTTAC
  AGTGCCTGAATCTGACACCATATGTGGCTGGTGATGTGTCCCCCGGTGGGCAGGCTGGACGGCGGGTGGC
  TGAATTGGACCCCGTCAACAGCTATCAGGCCTGGCAGCTAGCTACCAGGGGTAAGGTGGCTGAGCCGGCC
  GAGTACCTCAAGTACGGGACCTGGGACGAGGCGCTGGCAGGGGATGTAGCCTTTGACAAAGACAAGCGTG
  AGTATATACTGGTGAGCCAGGAAAAACGCAAGCGTGAACAGGATGCTCCGGCTACCCAAGCTCCCCCGAG
  GAAGAGGGCCAGTGTACCTGGGGACCCCATGCTTTCTGGCCACCTTGATCCCCGGCCAACCTCACGCCCC
  AACCGCAGGGCCTCGGCCACCACTGGCCAGGGCAAGTCCTCTGAGTGCTTCGAGTGCGGCAAGATCTTCC
  GCACCTACCACCAGATGGTGCTCCACTCCCGCGTGCACCGCCGTGCACGCCGTGACAGGGATCCTGAAGG
  GGACAGAGCAGCGCGTGCCCGTTGCGGTTCACTCAGCGAGGGTGACTCGGCTTCCCAGCCAAGCAGCCCT
  GGCTCAGCCTGCGCCATTGCTGACTCCCCTGGCCTGGCTGAGGAAGTGGTGGATGACAGTGGTGAAGAGG
  CTGTGCCTGAACCCGCATCAGGGGGCCAGCCTCGCCACTGCTGCTCTTCTGGAGAGGTGACACCCACAGC
  ACTCTCCAACGGAGACCAGAATCACAAGCTTGGAAATAACCTGCCAGAGAAAGACATCAGCGAGCCCAAG
  GTGGGGAGCGCCATGCCGTCTGTGTCCATACTTGAAAACAGCAGCAGAGAGACCACCAAGGGTCCCGAGC
  AGCATAGATACTCTCTCGACCTAAAGATGCCAGCATTTCACCCCAAGCAGGAGGTGCCCAGCACTACTGA
  CCGGGTAGACTTCCCTGCGAGCATGGAGATAACTAGCCTGCAGCACACCTTGGACAGCCAGGCCGGGCAT
  TCTAAAGAAAAGCTGTCCGATTTGCACAAGGAGCACTGCGGGGTAGGAAAGCGGGCATCCGCCCCGGACC
  TGGTCCCTCTGGACTTGAGCATGAGGTCGAGCAGGGATGAGCCCAGCGGTAAAGAAGCGTGCTCCCTGCA
  GGCGGCCCTGGTCATTCACCCGTGTCCTTACTGTACCCACAAGACCTACTACCCCGAGGTCCTGTGGATG
  CACAAGCGCATTTGGCACAGGGTCAGCTGCAGCTCTGTCGCACCTCCTTGGACTCAGCCCAGTGGCCACA
  AGAGCATTCGCAGCAACCTGGTTTTCCTCACTCGCAGTGGGCGCACAGGCCCTCCGCCTGCTCTTGGGGG
  CAAAGAGTGCCAGCCCCTGCTTCTTTCTCGCTTTGCCCGCACCCAGGTGCCAGGTGGGGCACCAGGATCA
  AAGGGCAGCTCCTCTCCCCTCGGGGTGACCACAAAAGCCGCTAGCATGCCTAAGAATAAGGAGAGCCATT
  CCGGGGGCCCCTGTGCTCTGTGGGCCTCTGGCCCCGATGGATATCGACAGACCAGAGCTGGACATGGCCA
  GGAGCCTCCCAGCGCTGCAGTGCAGGGGCCCCTGGCCAAACCCAAACAGGAGGGCAGCTCCAGGTTGGCA
  CCTTCCCCGGGCAGTGGGAGCCTCAGCAGAAGTACCACCCCCACACCCTCAGTGATAACCCGTGTTGGTG
  CCCAGCCCTCGGCCAATAGCAAACCAGTGGAGAAACTCGGGGGCCCAGCAGTGGGGACTGGCTTTACCCC
  TCCGAATAAGCACAGTGCCCCAGACTCTCTGAAAGCCAAATTCAGTCCTCAGCCTCAGGGTCAGCCTCCA
  TTGAAAGGTGAAGGAGGCTCTCCTCTACCTCCCCGAGAACCCTCTGTGAAGGCGGCCCAGGAGCTGAGAA
  CACTAGCCACCTGCGCAGCGGGGTCCAGAGGGGAGGCGGCCTTGCAAGCCCCACCTGGTGCGCCTCCCAC
  CTTAAACTCCGCCAAGCAGGAGCCGGCAGCTGAGGGGCAGGAGAAGCGCTTGGATATCCTCAGTATCTTT
  AAGACGTACATTCCAAAGGACTTCGCCACCCTCTACCAGGGCTGGGGTGTCAGCAGCCCTGGGCCCGAGC
  ACAGAGGGACATCACTAACAGGGACACCACGGACACAGGCCCACCAGGGAGACTTTGTCTGTGTGGAGTG
  CGGCAAGAGTTTCCATCAGCCCAGCCAACTCAGGGCCCACCTTCGGGCACACACAGTGGTGTTTGAGTGT
  GACGGTCCACGAGATTCTGAAGTTCACACAGCCTCCACGGATGCCCCTAAACAAGGGAGAGACCATACTA
  CCCCAGGGACTGTGCCAGCAGGGCCCCTCCGAAAGGGGATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177464
• Insert Size: 3474 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177464.1, NP_001170935.1
• RefSeq Size: 7703 bp
• RefSeq ORF: 3474 bp
• Locus ID: 329003
• UniProt ID: Q7TSH3
• Cytogenetics: 18 E3
• Gene Summary: Transcriptional regulator that binds to the promoter and activates the transcription of genes promoting brown adipose tissue (BAT) differentiation. Among brown adipose tissue-specific genes, binds the proximal region of the promoter of the UCP1 gene to activate its transcription and thereby regulate thermogenesis. May also play a role in the cellular response to replication stress (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.