Dock6 (BC057368) Mouse Untagged Clone

CAT#: MC223138

Dock6 (untagged) - Mouse dedicator of cytokinesis 6 (cDNA clone MGC:67290 IMAGE:6832553), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dock6
• Synonyms: 2410095B20Rik; 4931431C02Rik; C330023D02Rik; mKIAA1395
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCATGTAGCCAGCCTTGTGCACCGGAACCTGGAGGCTGTCCAAGACTCCCGTGGCCACTGCCCAC
  TGCTGGCCTCCTATGTCCACTACGCCTTTCGCCTCCCTGGTGGTGACCTCAGCCTGCCAGGCGAAGCCCC
  TCCAGCAACTGTGCAGGCTGCCACACTGGCCCGTGGCTCTGGTCGCCCCGCCAGCCTGTACCTGGCACGG
  TCTAAGAGCATCAGCAGCAGCAACCCTGACCTGGCTGTGGTCCCTGGCTCTGTGGACGATGAGGTGTCCC
  GCATCCTAGCCAGCAAGGGTGTCGATCGCTCACACTCCTGGGTGAATTCTGCTTATGCTCCGGGAGGCAG
  CAAGGCTGTGCTGCGGCGGGTGCCCCCATACTGTGGGGCTGATCCCAGACAGCTGCTGCACGAGGAGCTG
  GCTCTGCAGTGGGTGGTGAGCGGCAGTGCGGTTCGTGAGCTAGTCCTGCAGCATGCCTGGTTCTTCTTCC
  AGCTCATGGTAAAAAGCATGGAGCTCCATCTGCTTCTGGGCCAGCGACTGGACACTCCCCGCAAGCTGCG
  CTTCCCTGGGCGCTTCCTGGATGACATTGCCGCCCTGGTGGCTTCCGTGGGCCTGGAAGTCATCACCAGA
  GTCCATAAGGACATGAAGCTAGCTGAACGCCTCAACGCTAGCCTGGCTTTCTTCCTCAGTGACCTCCTGT
  CCATAGCGGACCGAGGCTACATTTTCAGCCTGGTACGCGCTCACTACAAGCAGGTGGCCACTCGGCTCCA
  GTCTGCCCCCAACCCAACTGCACTGCTCACACTACGAATGGACTTCACCCGCATCCTGTGTAGCCATGAG
  CACTATGTGACCCTTAACCTCCCCTGTTGCCCCCTGTCGCCCCCAGCATCCCCGTCCCCCTCGGTGTCCT
  CCACTACCTCCCAGAGCTCCACCTTCTCCAGCCAGGCCCCCGATCCGAAGGTGACCAGCATGTTCGAGCT
  GAGCGGGCCCTTCAGGCAGCAGCACTTCCTGTCCGGACTCCTGCTGACAGAGCTGGCCCTGGCCCTGGAT
  CCGGAAGCTGAGGGGGCATCCCTTTTGCACAAGAAGGCTATTAGTGCCGTGCACAGCCTGCTGTGCAGCC
  ACGATGTTGACTCCCGCTACGCAGAAGCTACCGTGAAGGCCAAGGTGGCCGAGCTGTACCTGCCCCTCCT
  CTCCCTTGCAAGGGACACACTGCCACAGCTGCATGGCTTTGCCGAGGGCTCAGGGCAGCGGTCAAGACTG
  GCTTCCATGCTGGACTCAGACACAGAAGGTGAAGGGGACATCGGAAGCACCATCAACCCATCTGTGGCCA
  TGGCCATCGCTGGGGGTCCCCTGGCTCCTGGCTCTCGCACCAGCATTTCCCAGGGACCTTCAACAGCAGC
  TCGCTCAGGCTGTCCTCTCTCTGCTGAATCCAGCCGGACCTTGCTGGTGTGTGTCCTGTGGGTCCTGAAG
  AATGCAGAGCCAACGCTCCTGCAGCGCTGGGCTGCTGACCTGGCCCTGCCCCAGCTGGGGCGACTCCTCG
  ACCTACTCTACCTCTGTCTGGCTGCCTTCGAGTACAAGGGGAAGAAAGCCTTTGAGCGGATCAACAGCCT
  CACGTTCAAGAAGTCTCTGGACATGAAAGCGCGGCTGGAGGAAGCCATCCTGGGCACCATTGGGGCGCGG
  CAGGAGATGGTGCGGCGGAGTCGGGAGAGAAGTCCATTTGGAAACCAAGAGAATGTTCGCTGGCGGAAGA
  GTGCCACACACTGGAGACAGACCTCAGACCGTGTGGACAAGACCAAGGATGAAATGGAACATGAAGCTCT
  GGTGGACGGAAACCTGGCGACAGAGGCCAGCCTGGTGGTTCTGGACACGCTGGAGACCATCGTGCAGACA
  GTGATGCTGTCCGAGGCCCGTGAGAGCATCCTGAGTGCCGTGCTGAAAGTTGTCCTCTACAGTCTTGGGA
  GCGCCCAGAGTGCCCTGTTCCTGCAGCATGGCCTGGCCACCCAGCGGGCCCTGGTCTCCAAGTTTCCGGA
  GCTGCTTTTCGAGGAAGACACGGAGCTTTGTGCCGACCTGTGCCTGAGACTTCTGCGACACTGTGGCAGC
  CGCATCAGCACCATCCGCATGCACGCCAGCGCCTCCCTCTACCTGCTTATGCGCCAGAACTTCGAGATTG
  GCCATAACTTTGCCCGTGTGAAGATGCTGGTGACCATGTCTCTGTCGTCCCTTGTGGGGACAACTCAGAA
  CTTTAGTGAAGAGCATTTGAGAAAGTCCCTCAAGACCATCCTGACCTACGCAGAGGAGGACATAGGGCTG
  AGGGACAGCACCTTCGCTGAGCAGGTCCAGGACCTCATGTTCAACCTGCACATGATCCTGACAGACACGG
  TGAAGATGAAGGAACACCAGGAGGACCCTGAGATGCTCATGGACCTCATGTACAGAATTGCTCGGGGATA
  CCAAGGCTCTCCAGACCTGCGGCTGACATGGCTGCAGAACATGGCTGGCAAACATGCAGAGCTGGGCAAT
  CATGCAGAGGCTGCCCAGTGCATGGTCCACGCCGCTGCCCTGGTGGCCGAATACCTTGCCCTCCTCGAGG
  ACAGTCGCCACCTGCCTGTGGGCTGTGTGTCCTTCCAGAATGTCTCATCCAACGTGCTGGAGGAGTCTGC
  CATCTCTGATGACATCCTGTCGCCAGATGAGGAGGGCTTCTGCTCTGGGAAGAACTTCACAGAACTGGGT
  CTGGTGGGGCTGCTGGAGCAGGCGGCCGGCTACTTCACCATGGGTGGTCTGTATGAAGCGGTGAACGAAG
  TCTACAAAAACCTTATCCCCATCCTTGAAGCCCACAGAGACTACAAGAAGCTGGCTGCGGTGCACGGGAA
  ACTGCAGGAGGCCTTCACCAAGATTATGCACCAGAGCTCTGGCTGGGAGCGTGTATTTGGGACATATTTC
  CGAGTGGGCTTCTATGGCACACGATTTGGTGACCTGGATGAACAAGAGTTTGTGTACAAGGAACCGTCAA
  TCACGAAGCTTGCAGAGATCTCACACCGGCTGGAGGAGTTCTATACGGAAAGGTTCGGGGATGATGTGGT
  AGAGATCATCAAAGATTCTAACCCAGTGGACAAGTCCAAGCTGGACCCACAGAAGGCGTACATACAGATA
  ACCTATGTGGAGCCACATTTCGACACTTATGAGCTCAAGGATCGGGTGACCTACTTCGATCGGAACTATG
  GGCTGCGGGCCTTCCTCTTCTGCACACCCTTCACACCAGATGGACGTGCGCACGGAGAGTTGGCCGAACA
  GCACAAACGCAAGACGCTGCTGAGCACGGAGCATGCCTTTCCCTACATCAAGACACGCATCCGAGTGTGC
  CACCGTGAGGAGACAGTGCTGACACCAGTGGAGGTGGCCATTGAGGACATGCAGAAGAAGACCCGGGAGC
  TGGCCTTTGCCACCGAGCAGGACCCTCCAGATGCCAAGATGCTGCAGATGGTTCTCCAGGGTTCTGTGGG
  ACCCACTGTGAACCAGGGTCCCTTGGAAGTGGCCCAGGTGTTTTTGTCAGAGATCCCAGAAGATCCCAAG
  CTCTTCCGACATCACAACAAACTCCGGCTCTGCTTCAAGGATTTCTGCAAAAAGTGCGAGGATGCACTGA
  GAAAGAACAAGGCCCTGATTGGCCCAGACCAGAAGGAGTACCACCGGGAGCTGGAGCGTCACTATAGCCG
  CCTGCGGGAGGCTCTGCAGCCTCTGCTTACCCAACGTCTGCCCCAGCTGCTGGCACCAAGTTCCACCAGC
  CTCAGGAGCTCCATGAACAGATCAAGTTTCAGGAAGGCTGACCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC057368
• Insert Size: 1626 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057368, AAH57368
• RefSeq Size: 4208 bp
• RefSeq ORF: 3827 bp
• Locus ID: 319899
• Cytogenetics: 9 A3
• Gene Summary: Acts as guanine nucleotide exchange factor (GEF) for CDC42 and RAC1 small GTPases (By similarity). Through its activation of CDC42 and RAC1, regulates neurite outgrowth in an vitro differentiation system.[UniProtKB/Swiss-Prot Function]