Vwa8 (NM_173758) Mouse Untagged Clone

CAT#: MC223124

Vwa8 (untagged) - Mouse RIKEN cDNA 1300010F03 gene (1300010F03Rik), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vwa8
• Synonyms: 1300010F03Rik; 4932416F07Rik; AI957255; Kiaa0564; mKIAA0564
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223124 representing NM_173758
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTCACGCCTCCTCCTCCTAGGAGCCCCGGGCGGCCTTGGGGACGTGGCCTCCCGGCGAGTGCGGC
  TGCTCTTGCGGCAGGTGTTGCGGGGCAGGCCGGGCGGGGACCAGCAGCGGCTGGAGGTCAGGCTGCTGCA
  CTCTGGGGCGACCGACTCAGGTGAAACGGTTAGTATTGGAGATGTGTCCTACAAGCTGAAAACTCCCAAG
  AATCCAGAACTTGTCCCACAGAACTACATTTCAGACTCTCCAGCTCAGTCCATAGTCCAGCATCTGAGAT
  GGCTGATGCAGAAGGATCTGCTGGGGCAGGATGTCTTTCTCATCGGACCTCCTGGGCCTCTCCGGCGCTC
  GGTGGCTATGCAGTACTTGGAGCTGACCAAACGAGAGGTGGAATACATCGCCCTGTCAAGGGACACCACT
  GAAACTGACCTCAAACAGCGCCGGGAGATCCGAGCTGGCACAGCCTTTTACATTGACCAGTGTGCGGTTC
  GGGCTGCCACAGAAGGCAGGACGCTGGTTTTAGAAGGCTTGGAAAAGGCGGAGAGAAACGTGCTTCCAGT
  ACTGAACAACTTGCTGGAGAACCGGGAGATGCAGCTTGAAGATGGGCGCTTCCTCATGTCTGCGGAGCGC
  TATGACAAGCTTCTCCAAGATCACACTAAAGAGGAGCTGGACGCGTGGAAGATTGTCCGAGTCAGTGAGA
  ATTTCCGAGTGATTGCCTTGGGCTTGCCAGTGCCCAGGTACTCTGGGAATCCATTAGACCCCCCTCTCCG
  GTCTCGATTTCAAGCCAGAGATATCTATTTTCTACCCTTTCAGGACCAACTAAAACTATTGTATTCAGTT
  GGAGCCAATGTTTCTGCTGAGAAGATTTCTCAGCTCTTGTCCTTTGCGACAACCCTCTGCTCCCAAGAAT
  CCTCTACGCTTGGGCTTCCAGACTTTCCCTTAGATAGTTTGCCAGAGGCAGTTCAAATCCTGGACTCCTT
  TCCCATGATGTCGATTGAACATGCACTCCAGTGGGTGTATCCTTACACTCTTTTACTCGGACACGAGGGG
  AAGATGGCCGTGGAGGGTGTTTTAAAACGCTTTGAGCTCCAAGGTTCTGGACATTCCCTGCTTCCTAAGG
  AGATTGTAAGAGTGGAGAGGATGACTGACAGCCACGGCTCCTATGCCCACGTGACAATCCGGGTCGCAGG
  AAAAGAGGTGACCATTAAGGTACCAGCGGGGACCAGAGCAGTAAACCAGCCTTGTGCTCCTGACCATTTC
  ATTCAGACTGTCAGTCACAAGCAATTACTGGCTGAGATGGTGCAGTCTCATATGGTGAAGGACATATGCT
  TAATTGGAGGGAAGGGCTGTGGGAAAACGGTCATCGCTAAGAACTTTGCTGCTCTCCTAGGATACAGCAT
  AGAACCCATCATGCTTTACCAGGATATGACTGCGCGGGACCTGCTACAGCAGCGGTACACGCTCCCCAAC
  GGCGACACCGCCTGGCGCTCCTCACCCCTTGTGAGTGCTGCCCGGGAAGGCAAACTGGTCCTGTTGGATG
  GTATCCACCGCGTCAACGCTGGCACACTGGCTGTATTGCAAAGACTGATCCATGACCGGGAGCTCAGCCT
  GTATGATGGATCTCGGCTGCTGAGGGAGGACAGGTACCTGAGCTTGAAGGAGAAGCTACAACTGACCGAT
  GAGCAGCTACAGAACAGGTCCATTTTTCCTATCCACCCTTCCTTCAGAATCATTGCGTTGGCAGAGCCCC
  CAATTGTTGGAAGCACAACACAGCAGTGGCTGGGACCAGAATTTTTAACTATGTTCTTTTTCCATCACAT
  GAAGCCTCTTGTGAAAAGTGAAGAAATCCAAGTGATAAAGGAGACGGTTCCAAATGTACCTCAGGAAGCA
  CTGGAGAAATTGCTGTCAGTCACTCACAAACTCAGGGAGACCCAGGATCCCACAGCACAGTCCTTGGCAG
  CCTCGCTTTCCACCCGTCAGCTCTTACGGATCTCTCGCCGGCTCTCGAAGTATCCCAGCGAAAATCTTCA
  TGATGCCATTACCAAAGCCTGTCTTTCCAGGTTCTTACCAAGCCTTGCTCAGTCTGCACTGGAGAAGAAT
  CTGGCAGATGCGGCAATAGAAACAAACACTGAGGACAGCCTGGAGCCAGAGCTGGAGAATTACAAGTGTG
  AAGTAGTCGCCGGGTCTCTGAAGATTGGCGCCGTCAGTGTGCCAGTGCACAATGCCCATGAGAAGATGAA
  AGTGCCTGACGTTCTTTTCTACGACAATGTTCAGCACATGGTAGTGATGGAAGACATGCTTAAAGACTTT
  GTCCTTGGCGAACACTTACTATTGGTTGGTAACCAGGGGGTAGGAAAGAACAAGATTGTGGACCGATTCC
  TTCACCTGCTCAACAGACCCCGAGAATATATACAGCTGCACAGGGACACCACAGTGCAGTCTCTGACTCT
  CCAGCCCACAGTGAAAGGTGGACTGATTGTATATGAAGACTCACCTCTGGTTAAAGCAGTAAAGTTGGGT
  CATATTCTAGTAGTAGATGAGGCAGACAAAGCCCCAACAAATGTCACCTGTATTTTGAAGACTCTGGTAG
  AAAACGGAGAAATGATTCTTGCGGACGGAAGACGTATTGTTGCAGATGCAGCAAATGTGGATGGAAGAGA
  GAACCTTGTCGCGATCCATCCTGACTTCAGGATGCTGGCTCTGGCCAACAGGCCTGGCTTCCCCTTCTTG
  GGCAATGATTTCTTTGGAACTTTAGGTGACATTTTTAGCTGTCATGCCATTGACAACCCCAAGCCCCACT
  CAGAGCTTTCGATGCTCAAACAGTACGGCCCAGACGTGCCTGAGCCCGTCCTGCAGAAGCTCGTGGCAGC
  CTTTGGAGAGCTGAGGAACTTGGCTGACCAAGGGATTATCAACTACCCTTATTCCACCAGAGAAGTGGTC
  AACATCGTCAAGCACTTACAGAAATTTCCCACTGAAGGCCTCTCCAGTGTGGTTCGGAATGTATTTGACT
  TCGATTCTTACAACAACGACATGAGGGAGATTTTGATGAACACTTTGCACAAATATGGGATCCCCATTGG
  AGCAAAGCCTACCAACGTGCAGCTGGCAAAGGAGTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_173758
• Insert Size: 3117 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173758.3, NP_776119.2
• RefSeq Size: 3486 bp
• RefSeq ORF: 3117 bp
• Locus ID: 219189
• UniProt ID: Q8CC88
• Cytogenetics: 14 D3
• Gene Summary: Exhibits ATPase activity in vitro.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents a shorter transcript with a distinct UTR and polyA signal and site, compared to variant 1. The resulting predicted protein (isoform 2) is much shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.