Frmd4a (NM_001177844) Mouse Untagged Clone

CAT#: MC223120

Frmd4a (untagged) - Mouse FERM domain containing 4A (Frmd4a), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Frmd4a
• Synonyms: 2700017I06Rik; 5330420J21; AI594879; C230040M21Rik; Frmd4; Gm13190; mKIAA1294
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223120 representing NM_001177844
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATTAGCCAGCATCAGTTCTATCTGGACAGAAAGCAGAGTAAGTCCAAGATCCATGCGGCCCGAA
  GTCTGAGCGAGATCGCCATTGACCTGACGGAAACAGGGACCCTGAAGACCTCAAAGCTGGCCAACATGGG
  GAGCAAGGGGAAGATCATCAGCGGGAGCAGTGGGAGTCTGCTGTCCTCAGGTTCTCAGGAATCAGATAGC
  TCGCAGTCGGCCAAGAAAGACATGTTGGCTGCCTTGAAATCCAGGCAGGAAGCTCTGGAGGAGACGCTGC
  GCCAGAGGCTGGAGGAACTGAAGAGATTGTGTCTCCGGGAAGCTGAACTCACAGGAAAATTGCCTGTGGA
  ATATCCCCTGGATCCAGGCGAGGAACCACCTATTGTTCGGAGGAGAATTGGAACAGCCTTCAAGCTGGAT
  GAACAAAAAATCCTGCCTAAAGGAGAGGAAGCTGAGCTGGAACGCCTGGAACGAGAGTTTGCGATTCAGT
  CCCAGATCACAGAGGCCGCCCGCCGTCTAGCCAGTGACCCCAATGTCAGCAAAAAACTGAAGAAGCAAAG
  GAAGACCTCTTATTTGAATGCACTGAAGAAGCTGCAGGAGATTGAAAATGCAATCAACGAGAACCGCATC
  AAGTCAGGGAAGAAACCCACCCAGAGGGCTTCACTTGTCATCGATGATGGAAATATCGCCAGCGAAGACA
  GCTCTCTCTCGGATGCCCTCGTTCTGGAAGATGAAGACTCTCAGGTTACCAGCACAATATCCCCCTTACA
  GTCGCCACACAAGGGACTCCCTCCTCGGCCACCTTCATCACACAACAGGCCTCCTCCCCCACAGTCCCTG
  GAGGGACTCAGGCAGCTGCACTATCACCGTACCGACTATGACAAGTCACCCTTAAAGCCCAAAATGTGGA
  GCGAGTCATCTTTAGATGAGCCTTATGAGAAGGTGAAGAAACGCTCCTCCCACGGCCATTCCAGCAGCCA
  CAAGCGCTTCCCCAGCACAGGAAGCTGTACCGAGGCAGGAGTAAGCAGCTCCTTGCAGAACAGTCCCATC
  CGAAGCCTCCCACATTGGAATTCCCAGTCTAGCATGCCATCCACCCCAGACCTGCGCGTCCGGAGTCCCC
  ACTATGTTCATTCCACAAGGTCAGTGGACATCAGCCCCACGAGACTGCACAGCCTTGCACTGCACTTTAG
  GCATCGAAGCTCCAGCCTGGAGTCCCAGGGCAAGCTCCTGGGCTCGGAGAACGACACCGGAAGCCCCGAC
  TTCTACACCCCGAGGACTCGTAGCAGCAATGGCTCAGACCCCATGGACGACTGCTCGTCCTGCACCAGCC
  ACTCGAGCTCAGAGCACTACTACCCAGCACAGATGAACGCCAACTACTCAACGCTGGCTGAGGACTCGCC
  GTCCAAGGCGCGGCAGCGACAGCGGCAGAGGCAGCGGGCAGCAGGCGCCCTGGGCTCAGCCAGCTCTGGC
  AGCATGCCCAACCTGGCGGCCCGCAGCGGGGCTGCAAGCACTGGAGGTGGCGTGTACCTGCACAGCCAGA
  GCCAGCCCAGCTCTCAGTACCGCATCAAGGAGTACCCACTATATATCGAGGGCAGTGCCACACCCGTGGT
  GGTACGCAGCCTGGAGAGCGACCAGGAGGGCCACTACAGCGTCAAAGCGCAGTTTAAGACCTCCAACTCT
  TACACAGCTGGCGGCCTGTTCAAAGAGAGCTGGCGCGGGGGTGGCGACGAGGGGGATGCAGGCCGCCTTA
  CACCATCTCGGTCTCAGATCCTGCGGACTCCTTCGTTGGGGCGTGACGGCGCCCACGACAAAGGCTCCGG
  CCGTGCCGCCGTGTCTGATGAGCTGCGCCAGTGGTACCAGCGCTCCACAGCCTCGCATAAGGAGCACAGC
  CGTCTGTCACACACCAGCTCCACCTCCTCGGACAGCGGCTCTCAGTACAGCACCTCTTCCCAGAGCACCT
  TCGTGGCGCACAGCAGGGTCACCAGGATGCCCCAGATGTGCAAGGCCACGTCAGCTGCCTTACCTCAAAG
  CCAGAGAAGCTCAACACCGTCCAGTGAAATTGGAGCCACCCCACCAAGCAGCCCCCACCACATCCTGACC
  TGGCAGACTGGGAGCTACAGTGACAGCTGCTTCCTGGATTCCTCCCTCTATCCAGAGCTAGCTGATGTCC
  AATGGTACGGGCAGGAAAAAGCCAAGCCCGGGACCCTCGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177844
• Insert Size: 2214 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177844.1, NP_001171315.1
• RefSeq Size: 5836 bp
• RefSeq ORF: 2214 bp
• Locus ID: 209630
• Cytogenetics: 2 A1
• Gene Summary: Scaffolding protein that regulates epithelial cell polarity by connecting ARF6 activation with the PAR3 complex (PubMed:20080746). Plays a redundant role with FRMD4B in epithelial polarization (PubMed:20080746). May regulate MAPT secretion by activating ARF6-signaling (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1.