Scaf8 (NM_134123) Mouse Untagged Clone

CAT#: MC223114

Scaf8 (untagged) - Mouse SR-related CTD-associated factor 8 (Scaf8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Scaf8
• Synonyms: A630086M08Rik; A930036P18Rik; AA408306; AI447644; AI448652; AU015035; mKIAA1116; Rbm16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223114 representing NM_134123
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCGTGAAGACCTTCAACAGCGAGTTGTATTCACTGAATGACTATAAGCCACCCATTTCTAAAG
  CAAAAATGACCCAAATTACAAAGGCAGCCATCAAAGCTATTAAGTTTTATAAACATGTGGTACAAAGTGT
  TGAGAAATTCATTCAGAAATGTAAACCAGAATACAAGGTACCTGGACTATATGTTATTGACTCCATTGTG
  CGACAATCCCGGCATCAGTTTGGTCAAGAAAAGGATGTGTTTGCACCCAGATTTAGTAATAACATCATTA
  GCACTTTTCAGAATTTATATCGTTGCCCTGGGGATGACAAGAGTAAAATAGTGAGAGTATTAAACTTATG
  GCAGAAGAATAATGTTTTTAAGAGTGAGATTATCCAGCCTCTTCTGGATATGGCGGCAGGGATTCCTCCC
  CCAGTTGTCACGCCTGTTTTGGCCAGCACCACTGCAGCTATGAGCAACACTCCAGGAACACCCGTGACAC
  CTGTTACTCCAGCCAATGTGGTCCAAGGCTTACCTGATCCATGGGTATCTCAGATAGCAAACACAGACAC
  ACTGGCAGCTGTTGCTCAGATCCTACAAAGTCCTCAAGGTCAACAGCTTCAGCAGCTAATACAAACCTTA
  CAGATACAGCAGCAGAAGCCTCAGCCTTCTATCCTGCAGGCCTTGGATGCTGGTCTTGTGGTTCAGTTAC
  AGGCACTCACTGCACAGCTCACAGCTGCAGCTGCTGCAGCCAACACACTTACGCCCTTGGATCAGGGAGT
  CTCTTTCAACAAGAAGTTGATGGATAGATTTGATTTTGGGGAAGATTCTGAGCATAGTGAAGAATCCAAA
  AAGGAAATGCCTACTCCTCAACTCTCTCACGTTTCAGAGTCTGTGAACAACTCCATCTTCCATCAGATAG
  CAGAACAGCTGCAGCAGCAGAACTTGGAGCAGTTGCGACAGCAGCTCCTGGAGCAGCAGCAGCCTCAGAA
  GGTAACTCCTCAAGATAGCCAGGAGGGGACATTTGGGTCTGAGCACTCAGCTTCTCCCTCACAAGGGAGT
  AGCCAACAGCATTTTCTGGAACCTGAAGCAAATTTAGATGATTCCATAGACATTCAGCAACAGGATATGG
  ATATTGATGAAGGGCAAGATGTTGTTGAAGAGGAGATCTTTGAACCAGAAGCTAAGAAAGTAGCTGTTCG
  CTCAAGATCAAGAACTCATTCACGATCTCGATCAAGGTCACCAAGAAAACGAAGGTCCAGGTCACGCTCT
  GGGTCCCGAAAGCGAAAGCACAGGAAGCGGTCTCGGTCCCACTCGAGAGAGAAAAAGAGAAAAGCCTCAC
  GCTCCTACTCCAGTGAGCGCAGAGCCAGGGAAAGGGAGAAAGAGCGCCAGAAAAAGGGCTTGCCTCCTGT
  CAGGTCAAAAACACTAAGTGTATGCAGCACTACTCTCTGGGTTGGCCAAGTGGACAAGAAGGCTACACAG
  CAAGATTTAACCAACCTTTTTGAGGAATTTGGACAAATTGAATCCATTAATATGATTCCACCCAGGGGCT
  GTGCATATGTCTGCATGGTTCATCGACAAGATTCATTTCGGGCTCTTCAGAAACTGAGTTCAGGATCCTA
  TAAAATTGGTTCCAAGGTCATTAAGATTGCCTGGGCTTTGAACAAAGGTGTAAAGACAGAATACAAACAG
  TTCTGGGATGTGGATCTTGGCGTCACATATATACCCTGGGAAAAAGTGAAAGTAGATGACTTGGATGGCT
  TTGCAGAGGGCGGCATGATTGATCAGGAGACTGTAAATGCTGAATGGGAAACTGTGAAAGCCTCAGAACC
  TGTTAAAGAGCCAGTGCAGACTGCACAGAGTCCAGCTCCAGTTGAGAAGGAGTCAGTGGTCACCACACAG
  GCAGAGGTTTTCCCTCCTCCTGTCGCCATGCTGCAGATTCCTGTAGCACCAGCTGTTCCTGCAGTTAGCT
  TAGTTCCTCCAGCGTTTCCTGTGTCGATGCCTGTCCCCCCTCCTGGATTCAACCCGATCCCTCCACCTCC
  TTTTCTACGAGCAAGTTTTAACCCTTCACAACCACCTCCTGGTTTTATGCCACCTCCAGTTCCCCCACCT
  GTGGTACCTCCCCCTGCAATCCCACCGGTAGTACCAACATCTTTAGTGCAACCACCATTGTCCATGACCC
  CTGAAGCTGTGAAAGATGTTGGATTTGGCAGCCTGGTTTTACCAAGTGGTTCTGTTGCTGGCAGTCTTGC
  TCCTTCAACTCTACCAGCTGGAAATGTTTTTAATCCTCCCAGTAAAGCAGAGCCTGAAGAAAAAGTACCT
  CACCTTATAGAGCACCAGATTCCTTCTGGTGAGAACACAAGACCAGTGATTCCAAGTGATATTCCAAGTA
  GTGCTGCAATGTTAGCACAGCCGCCTGGGGCCTCAAGCACCTCTGGGATCCTGTGCGTGCAGAGACCAAA
  TGTATCAAGTAATTCTGAAATTCTGGGGGTTCGTCCAGCTAATGTTTCCAACAGTGCTGCAATTATGGGA
  GCCCAGCCACCAAATATTCTAAATAACTCTGGAATTTTGGCCATACAGCCACCAAATGTGTCCAGTGGCT
  CTGGACTTCTTGGGGTACTGCCTCCAAACTTGCCTAACAACTCTGGACTTGTAGGACTACAGCCACCAAA
  TGTTACAAGTCCTGCTGGACTTTTGGGAACACAGCCACCAATTGGACCTCAAAACTTACCACCTTTAGCC
  ATCCCTGCTCAAAGGATGCCCGCGTTGCCAATGTTAGACATTCGCCCAGGACTGATAGCACAGGCTCCCG
  GGCCACGATTCCCTTTACTACAGCCTGGAATTCCACCACAACGCGGTATCCCTCCCCCATCGGTACTTGA
  TGCAGCTCTTCATCCTCCACCCCGCGGACCTTTTCCTCCAGGAGATCTTTTCAGTCAGCCAGAAAGACCT
  TTTCTGGCCCCTGGAAGACCAAGTATAGACAATGTTCCCAACCCAGATAAAAGAATACCACTTGGGAATG
  ACAATATTCAGCAGGAAGGGGATAGAGATTACCGCTTTCCTCCTATAGAAACCAGGGAGGGCATTACCAG
  ACCTCCTCAGGTGGATGTTAGGGATGTGGTTGGGCGACGTCTAGATCCCAGAGAAGGCCCTGGAAGGCCT
  CCATTAGATGCTAGGGATCATTTTGGAAGACCTCCTGTGGACATGAGGGAGAATCTTGTGAGGCCAAGTC
  TAGACCACCTTGGTCGAAGAGACCACTTTGGCTTTCCCCCAGAGAAGCCTTGGGGGCCTAGAGATTTTGA
  TGAGAGAGAGCATCGAGTTCTGCCTGTCTTTGGTGGTCCAAAAGGCTTACATGAAGAAAGAGGTAGATTT
  CGGGCTGGAAATTACCGATTTGATCCTAGAAGTGGTCCTTGGAACAGAGGATTTGGTCAAGAAGTTCACA
  GAGATTTTGATGACCGCAGAAGACCCTGGGAGAGGCAGAGGGATAGGGATGACAGAGATTTTGATTTCTG
  CAGAGAAATTAATGGAAATCGTCTTGGACGAGATAGAATTCAAAACACTTGGGTTCCCCCTCCTCATGCT
  CGGGTTTTTGATTATTTCGAAGGGGCCACTTCTCAACGGAAAGGTGATAATGTGCCTCAAGTTAATGGTG
  AAAATACAGAGAGACATGCTCAGCCACCGCCTTTACCAGTACAGAAGGACCCTGAACTCTATGAGAAACT
  GGCGTCTTCAGGTGACGTAGACAAGGAGGAGAGCGGCACAGTTGCTGGTGTAGAGAGTGAAGCGGTGGTA
  GAAAGCACAGAGACTGAGGGGACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134123
• Insert Size: 3807 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134123.3, NP_598884.2
• RefSeq Size: 4892 bp
• RefSeq ORF: 3807 bp
• Locus ID: 106583
• UniProt ID: Q6DID3
• Cytogenetics: 17 A1
• Gene Summary: Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF4, also acts as a positive regulator of transcript elongation.[UniProtKB/Swiss-Prot Function]