Efl1 (NM_001159672) Mouse Untagged Clone

CAT#: MC223112

Eftud1 (untagged) - Mouse elongation factor Tu GTP binding domain containing 1 (Eftud1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Efl1
• Synonyms: 4932434J20Rik; 6030468D11Rik; AI451340; AU019507; AU022896; D7Ertd791e; Eftud1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223112 representing NM_001159672
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTCAGTGGTGTTGATAAGATGATTCGACTTCAGAAGAACACTGCCAACATTAGGAATATCTGTG
  TTCTGGCTCATGTTGACCACGGAAAAACTACTCTTGCTGATTGTCTTATATCCAGTAATGGAATCATCTC
  CAGCCGCCTGGCAGGCAAGTTAAGGTATATGGACAGCAGAGAAGATGAACAGGTCAGAGGGATCACCATG
  AAGTCCAGCGCCATCTCACTACATTATGCAGAAGGTCATGAGGAGTACCTGATTAATCTGATAGACTCTC
  CTGGACACGTGGACTTCTCCTCAGAAGTATCAACAGCGGTTCGCATTTGTGATGGATGCATCATTGTGGT
  GGATGCTGTGGAGGGGGTCTGTCCACAGACACAGGCAGTTCTTCGACAAGCCTGGCTTGAAAACATCCGC
  CCTGTTTTAGTGATCAATAAGATAGACCGCCTGATAGTGGAACTAAAATTCACCCCACAAGAGGCCTATT
  CTCACCTCAAGAATATTTTAGAACAGATTAACGCACTTACAGGAACTCTTTTTACTTCTAAAGTCCTAGA
  AGAAAGAGCAGAGAGAGAGACAGAATCCCAGGCGAAGCCACATTCTGAGCAAGGAGAGCAAGTGTATGAC
  TGGAGCGCGGGCTTGGAGGACGTGGACGACTCGCAGCTGTACTTCTCGCCAGAGCAGGGCAATGTGGTGT
  TTACCAGTGCGATAGATGGCTGGGGCTTTGGAATTGAGCACTTTGCCAGAATCTATAGTCAAAAAATTGG
  CATCAAGAAGGAAGTTCTTTTGAAAACCTTGTGGGGAGATTATTATATAAATATGAAGGCTAAGAAGATT
  ATGAAAGTTGATCAGGCCAAAGGAAAGAAACCTTTATTTGTACAGTTGATCCTGGAAAATATATGGAGTT
  TGTATGATGCAGTCTTGAAAAAGGACAAAGAAAAAATTGATAAAATTGTGACTTCTTTAGGATTAAAAAT
  TGGAGCCCGGGAGGCACGACATTCAGACCCTAAAGTCCAGATCAATGCCATTTGCAGCCAGTGGCTCCCC
  ATATCCCACGCTGTCCTCGCTATGGTGTGTCACAAACTGCCCAGTCCCCTTGACATGACGTCGGAGCGAG
  TAGAGAAACTGCTGTGCACAGGATCCCAGACGTTTGAGTCTCTGCCACCGGAGACCCAAGCCCTGAAAGC
  AGCTTTTATGAAATGTGGAAGTGAGGATACTGCTCCAGTTATTATCTTTGTTTCCAAAATGTTTGCAGTT
  GATGTGAAAGCCTTGCCTCAGAATAAGCCAAGGCCTCTCACTCAAGAAGAAATGGCCCAGAGACGGGAAC
  GGGCAAGACAGAGGCACGCAGAGAAGCTTGCAGCAGCCCAGGGGCAAACATCCCAGGGACCCACCCAGGA
  TGGAGGTGCCCTTGAAACAAGTCCCCATGAAGATGAGCCAAGAGGTGATGAACCAGATGTGGCAAGTGTA
  AGCCGCCAGCCTGTTTCCCAGGAGGAAAGCAGCCAAGAGGCCTTCATCGCATTTGCTCGTGTGTTCAGTG
  GCATAGCTCGAAGAGGAAAGAAAATTTTCGTCTTAGGACCCAAGTACAGCCCTGTTGATTTTTTACAGCG
  GGTTCCACTAGGCTTCTCAGCTCCACTAGAGGACCTCCCTCCTGTGCCCCACATGGCGTGCTGTACTCTG
  GAGAACCTGTACCTTCTGATGGGAAGGGAGCTGGAAGATCTGGAAGAAGTCCCCCCAGGAAACGTGCTGG
  GAATTGGAGGCCTCCAGGACTTTGTGCTAAAATCTGCAACTTTGTGCAGCCTGCCGTCCTGCCCACCGTT
  TATTCCCCTCAACTTTGAAGCCACCCCGATTGTGAGAGTTGCTGTTGAACCAAAGCACCCAAGTGAAATG
  CCTCAGCTTGTGAAAGGAATGAAGCTGCTAAACCAGGCTGACCCCTGTGTCCAGGTTTTAATTCAGGAAA
  CAGGAGAGCATGTCCTGGTCACGGCCGGGGAGGTCCACCTTCAGCGATGTTTGGATGACTTACGAGAAAG
  GTTTGCAAAGATCCACATCAGTGTATCTGAACCCATTATTCCATTCAGAGAAACGATCACAAAACCTCCC
  AAAGTTGACATGGTCAATGAAGAAATAGGCAGGCAGCAAAAAGTTGCTGTCATACACCAAACAAAAGAAG
  AGCAAAGCAAAATCCCAGAGGGGATCCACGTGGACTCGGATGGGCTTATCACCATACCGACTCCCAACAA
  ACTGGCCACTCTCAGTGTCCGTGCCATACCGCTTCCAGAAGAAGTCACCAGGATTCTGGAAGAAAACAGC
  GACTTGATTCGTTCCATGGAGCTTTTGACATCCTCCTTGAACGAGGGTAGAAATACCCAAGCCATTCATC
  AGAAGACCCAAGAGAAAATATGGGAATTCAAAGGAAAGCTAGAGAAACATCTAACAGGGAGGAAATGGAG
  GAACACTGTTGACCAGATCTGGTCATTTGGCCCAAGGAAATGTGGGCCCAACATCCTAGTCAGTAGAAGT
  GAAGATTTCCAGAACTCAGTATGGTCTGGCCCAGCTGGCAGAGAATCAAAAGAGGCCAGCAGATTCCGAG
  ACTTTGGCAATAGCATTGTGAGTGGCTTCCAGCTTGCGACCCTCTCTGGCCCCATGTGTGAGGAGCCGCT
  CATGGGTGTCTGTTTTGTTCTAGAAAAGTGGGAGCTAAATAAATGTGCAGAACAAGGAGCAAGTGATAAA
  CAGCACCAAGGTCAGTGTGATCTGGCAGGAGAGGGACAGGGGGGAGGTAAAACCTGTCACGTTGGAGATG
  AAAACCAAGAGCAACAAGATGTCTGCTCGGAGCCCTTTGAAGAGACTTCCCAGAAAGGAGACTCTCCGGT
  TATTGACTGCTATGGACCCTTCTCCGGGCAGCTAATTGCTACCATGAAAGAAGCCTGCCGCTACGCTCTG
  CAGGTGAAACCTCAACGCCTGATGGCAGCTATGTACACATGTGACATCATGGCCACCAGTGATGTCCTCG
  GCCGAGTCTATGCCGTGCTGTCAAAGCGAGAAGGTCGAGTGCTTCAGGAAGAGATGAAGGAAGGGACGGA
  CATGTTCATCATCAAGGCTGTGCTGCCTGTAGCGGAAAGCTTTGGCTTTGCGGATGAGATCAGGAAGAGG
  ACCAGCGGACTGGCCAGCCCACAGCTGGTGTTCAGCCACTGGGAGGTCATTCCCAGCGACCCGTTCTGGG
  TGCCGACCACGGAGGAGGAGTACCTGCACTTTGGGGAGAAGGCGGATTCCGAGAACCAGGCCCGGAAGTA
  CATGAATGCCGTGCGGAAGCGGAAGGGCCTCTATGTGGAAGAAAAGATTGTGGAACACGCAGAAAAGCAG
  AGAACCCTCAGCAAAAACAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159672
• Insert Size: 3384 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159672.1, NP_001153144.1
• RefSeq Size: 3608 bp
• RefSeq ORF: 3384 bp
• Locus ID: 101592
• UniProt ID: Q8C0D5
• Cytogenetics: 7 47.25 cM
• Gene Summary: Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.