Phlpp (BC059254) Mouse Untagged Clone

CAT#: MC223110

Phlpp (untagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phlpp
• Synonyms: SCOP, mKIAA0606
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC059254
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTTGCCAGTGAACCGATGGACAAGACGACAGGTCATTCTGTGCGGGACGTGCTTGATAGTGTCGT
  CCGTGAAAGACAGCGTGAGTGGGAAGATGCATGTCCTGCCCCTCATTGGTGGAAAAGTGGAGGAAGTGAA
  AAAGCACCAGCACTGTTTAGCGTTCAGCTCTTCGGGGCCTCAAAGCCAGACTTACTACATCTGCTTTGAT
  ACTTTCACGGAGTACTTACGGTGGCTGCGGCAAGTCTCCAAGGTTGCATCACAGCGTATAAGCTCAGTAG
  ACCTCTCCTGTTGTAGCCTTGAACATCTGCCTGCCAACCTCTTTTACAGCCAAGACCTTACTCATCTCAA
  CTTAAAACAGAACTTCCTAAGGCAAACCCCCACCCTCCCAGCTGCCAGAGGACTTGGTGAACTGCAAAGG
  TTCACCAAATTGAAGAGCCTTAACCTTTCCAATAACCACCTAGGAGCCTTTCCGTCAGCAGTCTGCAGCA
  TTCCAACCCTAGCAGAGCTGAATGTATCTTGCAATGCCCTGCGAGAAGTCCCAGCAGCTGTTGGAGATAT
  GCAGAACTTGCAGACGTTTCTGCTGGATGGAAATTTTCTCCAGTCCCTTCCTGCTGAACTGGAGAGCATG
  CACCAGCTCAGCTATCTGGGTCTTTTCTTTAATGAATTCACTGACATTCCAGAGGTATTGGAGAAGCTGA
  CTGCTGTGGATAAGCTGTGTATGGCTGGGAACTGTGTGGAGACCCTCAGACTACAGGCCTTAAGAAGAAT
  GCCTCATATTAAACATGTGGACCTAAGACTGAACATACTCAGAAAGCTTATGGCAGATGAGGTGGACTTT
  GTGCAACATGTCACTCAGCTTGACCTGCGAGACAATAAGCTTGGTGATCTAGATGCTATGATCTTCAACA
  ACATAGAAGTTCTGCACTGTGAGAGGAATCAGCTGGTGACATTGAACGTTTGTGGCTATTTCCTAAAGGC
  ACTCTATGCTTCTTCTAATGAACTTGCTCAACTTGATGTCTACCCAGTTCCGAATTATCTCTCTTACATG
  GATGTCTCAAGAAACTGCCTAGAAAGTGTGCCTGAGTGGGTATGTGAAAGCCGGAAATTAGAAGTTTTGG
  ATATTGGCCATAATCAAATATGTGAACTTCCTGCCCGCCTGTTTTGTAATAGTAGTCTCCGGAAATTGCT
  AGCAGGACACAACCGGTTGGCAAGGCTTCCTGAAAGGCTGGAGAGAACTTCTGTGGAGGTCTTGGATGTC
  CAGCACAACCAGATCACTGAGCTCCCACCAAACCTCCTCATGAAGGCTGACAGCCTGAGATTCCTGAATG
  CATCTGCAAACAAACTGGAAACCCTGCCTCCAGCCACACTTTCTGAGGAGACGAGCAGTATATTACAGGA
  GTTGTACCTGACAAACAACTGCCTCACGGATAAGTGTGTGCCCTTGTTAACAGGGCACCCCCGTCTGAAG
  ATCCTACACATGGCCTATAACCGGCTTCAGAGCTTCCCGGCAAGTAAAATGGCAAAACTGGAGGAACTTG
  AAGAAATTGATATCAGTGGGAATAAGCTGAAAGCCATCCCCACAACAATCATGAACTGCAGACGCATGCA
  CACCGTGATTGCTCACTCCAATTGTATCGAAGTCTTTCCTGAAGTTATGCAGCTCCCAGAAGTCAAGTGT
  GTAGATCTGAGCTGTAATGAGCTGAGTGAAATCACGTTACCAGAAAACCTGCCACCTAAGCTGCAGGAGC
  TAGACCTGACTGGAAATCCACGCCTTGCCCTGGACCACAAAAGCCTGGAGCTGCTCAATAACATTCGCTG
  CTTCAAGATTGACCAGCCGTCAGCAGGAGATGCATCAGGAGCCCCAGCAGTATGGAGTCATGGTTACACT
  GAAGCATCGGGAGTAAAAAACAAGCTGTGTGTCGCAGCCCTGTCTGTAAATAACTTCCGTGACAACCGAG
  AGGCCCTCTATGGTGTGTTTGATGGAGACCGGAATGTGGAGGTGCCCTACCTTCTCCAGTGCACCATGAG
  TGACATTTTGGCTGAAGAGCTTCAGAAAACGAAAAATGAAGAAGAATACATGGTCAATACATTCATAGTC
  ATGCAAAGGAAACTGGGAACTGCTGGGCAGAAACTTGGTGGTGCTGCTGTCCTCTGCCACATCAAGCCTG
  ACCCTGTGGACCTGGGAGGATCCTTTACCCTGACCTCTGCTAATGTTGGCAAGTGCCAAACGGTTCTCTG
  TCGAAATGGGAAGCCACTGTCTCTGTCCAGGTCATACATCATGAGCTGTGAAGAAGAGCGGAAGAGGATT
  AAGCAGCACAAAGCCATCATCACTGAGGATGGCAAGGTCAATGGAGTGACAGAGTCCACGCGCATCCTGG
  GCTACACCTTCCTCCATCCCAGTGTGGTGCCTCGCCCCCATGTGCAGTCGGTGCTTCTGACTCCACAGGA
  TGAGTTTTTCATCCTGGGCAGCAAAGGGCTGTGGGACAGCCTATCCATTGACGAGGCTGTGGAAGCTGTA
  CGCAATGTGCCAGATGCTCTGGCTGCTGCTAAGAAGCTCTGCACCCTGGCTCAGAGCTATGGCTGCCATG
  ACAGCATCAGTGCTGTGGTGGTACAGCTAAGTGTCACCGAAGACAGCTTCTGCTGCTGTGAGCTCAGTGC
  TGGAGGGAGCATGCCACCACCCAGCCCCGGAATCTTCCCACCATCCGTCAACATGGTCATCAAGGACCGA
  CCCTCAGATGGGCTGGGTGTGCCATCCTCCAGCAGTGGCATGGCATCTGAGATCAGCAGTGAACTGTCTA
  CCTCCGAGATGAGTAGTGAGGTGGGCTCCACAGCCTCTGATGAGCCCCCGTCTGGAGTCCTGAATGAGAG
  CAGCCCTGCCTACCCCAACGAGCAGCGCTGCATGCTCCACCCTGTCTGCCTGTCTAACTCCTTCCAACGT
  CAGCTGTCCAGCGCTACTTTCTCCAGCGCGTTCTCTGACAACGGCCTTGACAGTGACGATGAAGAACCCA
  TTGAGGGGGTGTTCAGCAACGGCAGCCGGGTTGAGGTGGAAGTAGACATCCACTGCAGCAGGGCCAAGGA
  AAAGGAGAGACAGCAGCACCTACTTCAGGTGCCAGCTGAGGCCAGTGATGAGGGCATTGTCATCAGTGCC
  AATGAGGATGAGTCAGGTCTGTCCAAGAAGGCAGACTTTTCTGCTGTGGGAACCATTGGACGACGCAGGG
  CTAATGGCTCTGTAGCTCCCCAGGAAAGGAGCCATAATGTGATAGAGGTTGCTGCAGATGCGCCTCTCCG
  GAAGCCAGGAGGCTATTTTGCAGCCCCTGCTCAGCCAGATCCAGATGATCAGTTTATCATCCCCCCAGAG
  CTGGAAGAGGAAGTCAAAGAAATCATGAAACATCACCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
  TGCCACCACCACCCCAGCCGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCG
  GCACTTCCAAATGGATCACCTGCCAGACTGTTACGATACACCGCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC059254
• Insert Size: 3549 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC059254, AAH59254
• RefSeq Size: 4783 bp
• RefSeq ORF: 3548 bp
• Locus ID: 98432
• Cytogenetics: 1 E2.1
• Gene Summary: Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (By similarity). Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity). Involved in the hippocampus-dependent long-term memory formation (PubMed:17382888). Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (PubMed:20080691). Involved in development and function of regulatory T-cells (PubMed:21498666).[UniProtKB/Swiss-Prot Function]