Dhx30 (BC016202) Mouse Untagged Clone

CAT#: MC223099

Dhx30 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (cDNA clone MGC:27662 IMAGE:4527765), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dhx30
• Synonyms: HELG, Ddx30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC016202
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCCAGGCGGCTCATGGCGCTGGCTGCCGGCGTCTCTCCGCGCCTCCGGCCGCCGGATCCCC
  TTGTCGCTTCCGGGCGACAAGGATGTTCGCGCGGCTTCTCATCAAGCTTTGTCCGCTCTGATGGCACTCA
  GGAAGCTGCCGAGGTCGAATCAGAGGTGGCCCCTAGCGAGCCTGGGGAGGGTGACGGAAGCATGGTGAAC
  GCTTCTAGGGACCTATTAAAAGAGTTTCCGCAGCCTAAAAACCTTCTCAACAGCGTGATTGGAAGAGCCC
  TTGGCATCTCACATGCAAAAGACAAGTTAGTCTATGTGCACACGAATGGACCGAAGAAAAAGAAAGTCAC
  CCTGCACATAAAGTGGCCCAAGAGCGTGGAGGTGGAAGGCTATGGCAGCAAGAAGATTGATGCTGAGCGT
  CAGGCTGCAGCAGCTGCCTGCCAACTCTTCAAGGGCTGGGGTCTGCTGGGACCACGGAATGAGCTGTTTG
  ATGCAGCTAAATACCGAGTGCTAGCTGATCGTTTTGGGTCTCCAGCTGACAGCTGGTGGCGCCCAGAACC
  CACCATGCCTCCAACTTCCTGGCGGCAGCTGAATCCTGAGAACATTCGGCCAGGGGGTCCTGCAGGACTA
  TCCCGATCCTTAGGCCGAGAGGAAGAGGAAGATGAGGAGGAAGAGCTAGAAGAGGGGACCATTGATGTGA
  CAGAGTTTCTGTCTATGACCCAGCAAGACTCCCACAACCCACTCAGGGACTCAAGGGGGGGCTCCTTTGA
  AATGACAGATGATGACAGTGCTATCAGAGCTCTGACCCAGTTTCCACTTCCCAAGAACCTCCTGGCCAAA
  GTGATTCAGATTGCAACCTCCTCCTCCACAGCTAAGAATCTCATGCAGTTCCATACTGTGGGTACCAAGA
  CCAAGCTGGCTACACTCACTCTGCTCTGGCCCTGTCCCATGACCTTTGTTGCCAAAGGGCGACGCAAAGC
  TGAGGCTGAGAATAAGGCAGCAGCCTTGGCTTGCAAGAAACTGAAGAGCCTTGGCCTTGTGGACAGGAAC
  AATGAGCCGCTTACCCATGCCATGTACAACCTGGCCTCCCTGCGTGAGTTGGGTGAGACCCAGCGCCGGC
  CATGTACCATCCAGGTGCCTGAGCCCATCCTTCGCAAGATAGAGGCCTTCCTGAGTCATTACCCGGTGGA
  CAGCTCATGGATTTCCCCAGAACTCCGACTGCAGAGTGATGACATCTTGCCCTTAGGCAAGGACTCAGGG
  CCCTTGAGTGACCCTATCACAGGCAAGCCATACATGCCCCTGTCAGAAGCAGAGGAGGTGCGCTTAAGCC
  AGAGCCTGCTAGAGCTGTGGCGGAGGAGAGGGCCGATCTGGCAGGAGGCCCCGCAGCTACCTGTAGACCC
  TCATCGGGACACTATCCTCAGTGCCATTGAACAACACCCAGTGGTAGTCATCTCTGGGGACACAGGCTGT
  GGGAAGACCACACGTATCCCTCAGCTGCTATTGGAGCGCTATGTGACTGAGGGTCGAGGTGCCCGCTGCA
  ATGTGATCATCACACAACCTCGCCGGATCTCAGCTGTGTCTGTGGCACAGCGGGTCAGCCATGAACTGGG
  CCCCTCCTTGCGCCGGAATGTGGGCTTCCAGGTACGCTTGGAAAGTAAGCCCCCAGCCCGAGGTGGGGCG
  CTGCTCTTCTGCACTGTGGGTATCCTGCTTCGGAAGCTGCAGAGCAACCCCAGCCTGGAGGGTGTGAGCC
  ATGTCATTGTGGATGAGGTCCATGAGCGGGATGTGAACACAGACTTCCTGCTGATTCTGCTCAAGGGCCT
  GCAGCGGCTCAACCCAGCTCTTCGGCTGGTGCTCATGAGTGCTACAGGAGATAATGAGCGCTTTTCCCGA
  TACTTTGGGGGCTGCCCTGTTATCAAGGTACCTGGCTTCATGTATCCCGTCAAGGAACACTACCTGGAGG
  ACATCTTGGCCAAGCTGGGTAAACATCAGTACCCACACCGGCATCGGCACCATGAGTCGGAAGATGAATG
  TGCACTTGATTTGGACCTTGTGACTGACCTGGTTCTGCATATCGATGCCCGTGGGGAACCAGGTGGGATC
  CTGTGCTTTCTACCTGGCTGGCAAGAAATCAAAGGAGTGCAACAACGGCTCCAGGAGGCCCTGGGCATGC
  ATGAAAGCAAGTACCTCATCTTACCAGTGCACTCCAATATCCCCATGATGGACCAGAAGGCCATATTCCA
  ACAGCCTCCACTTGGGGTACGCAAGATTGTATTGGCCACCAACATTGCTGAAACCTCCATCACAGTTAAC
  GACATTGTACATGTCGTGGACAGCGGTCTGCACAAGGAGGAACGCTATGACCTGAAGACCAAGGTGTCCT
  GCCTGGAGACTGTGTGGGTGTCGAGAGCAAATGTCATTCAGCGCCGGGGCAGGGCAGGCCGCTGCCAGTC
  AGGTTTTGCCTACCACTTGTTCCCGAGGAGCCGGCTGGAGAAAATGGTTCCTTTCCAAGTGCCAGAGATC
  CTGCGCACACCTCTTGAGAACCTGGTGCTGCAAGCCAAAATCCATATGCCTGAGAAGACGGCAGTGGAGT
  TCCTCTCTAAGGCTGTGGACAGTCCAAATATCAAGGCAGTGGATGAGGCCGTGATCCTGCTCCAGGAGAT
  TGGGGTGCTGGACCAGCGGGAGTATCTGACCACCTTGGGACAGCGCCTGGCCCACATCTCTACTGACCCC
  CGACTGGCCAAGGCCATAGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTGCTGGTGGTTGTTTCCT
  GCCTTACCCGGGACCCCTTCAGCAGCAGTTTGCAGAACCGGGCAGAAGTAGACAAGGTGAAGGCATTGCT
  GAGCCATGACAGTGGCAGTGACCATTTGGCCTTCGTGCGGGCTGTGGCTGGCTGGGAGGAGGTACTGCGC
  TGGCAGGACCGTACCTCCAGGGAAAACTACCTGGAAGAAAACCTTCTGTATGCCCCCAGCTTGCGCTTCA
  TCCACGGGCTCATCAAGCAGTTCTCAGAGAACATTTATGAGGCTTTCCTAGTGGGGAAGCCCTCTGACTG
  CACACTGCCCTCTGCTCAGTGCAATGAGTACAGCGAGGAAGAGGAGCTGGTGAAGGGTGTGCTGATGGCT
  GGCCTCTACCCCAACCTCATCCAGGTGAGACAAGGTAAGGTTACTCGGCAAGGCAAGTTCAAACCCAACA
  GTGTCACTTACAGGACCAAATCTGGCAACATCTTGCTGCATAAGTCAACCATTAACAGGGAGGCTACCCG
  GTTACGGAGCCGATGGCTGACATATTTCATGGCCGTCAAGTCCAATGGTAGCGTCTTTGTTCGAGATTCC
  TCCCAGGTGCACCCACTAGCTGTGTTGCTCCTAACAGATGGGGACGTGCACATCCGAGATGATGGGCGTC
  GGGCCACCATCTCATTGAGTGACAGCGACCTGCTTCGGCTGGAAGGTGATTCACGAACTGTGCGGTTGCT
  AAGGGAGTTTCGTCGAGCCCTAGGACGGATGGTGGAGCGGAGCCTCCGCAGCGAGTTAGCTGCACTTCCT
  CTTAGTGTGCAGCAAGAACACGGGCAGCTGCTTGCACTGCTGGCAGAGTTGCTTCGAGGACCTTGTGGCA
  GCTTTGATATGCGCAAGACAGCTGATGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC016202
• Insert Size: 3672 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC016202, AAH16202
• RefSeq Size: 3768 bp
• RefSeq ORF: 3671 bp
• Locus ID: 72831
• Cytogenetics: 9 F2
• Gene Summary: RNA-dependent helicase (PubMed:25219788). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (By similarity). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (By similarity). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (PubMed:25219788).[UniProtKB/Swiss-Prot Function]