Ulk2 (BC053029) Mouse Untagged Clone

CAT#: MC223082

Ulk2 (untagged) - Mouse Unc-51 like kinase 2 (C. elegans) (cDNA clone MGC:62255 IMAGE:5709559), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ulk2
• Synonyms: Unc51.2, mKIAA0623
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053029
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGGTGGGCGACTTCGAGTACTGCAAGCGGGACCTCGTGGGACACGGGGCCTTCGCTGTGGTCT
  TCCGGGGGCGGCACCGCCAGAAAACTGATTGGGAGGTGGCTATTAAAAGTATTAATAAAAAGAACTTGTC
  AAAATCACAAATTCTGCTTGGAAAGGAAATAAAAATCTTAAAGGAGCTTCAGCATGAAAACATCGTAGCG
  CTCTATGATGTTCAGGAATTGCCCAACTCTGTCTTTCTGGTGATGGAGTATTGCAATGGTGGAGACCTGG
  CAGATTATTTGCAAGCTAAAGGAACTCTGAGTGAAGATACTATCAGAGTGTTTCTCCATCAGATTGCGGC
  AGCCATGCGAATCCTGCACAGCAAAGGGATAATCCACAGGGATCTCAAACCACAGAATATCCTGTTGTCT
  TATGCCAATCGAAGGAAGTCGAATGTCAGTGGTATTCGTATTAAAATAGCTGATTTTGGTTTCGCACGGT
  ACCTACATAGTAACACAATGGCAGCGACACTGTGTGGATCCCCAATGTACATGGCTCCCGAGGTTATTAT
  GTCTCAACATTATGATGCTAAGGCAGATTTATGGAGCATAGGAACAGTGATCTATCAATGCCTAGTTGGA
  AAACCACCTTTTCAGGCTAATAGTCCTCAGGACCTAAGGATGTTTTATGAAAAAAACAGGAGCTTAATGC
  CTAGTATTCCCAGAGAAACATCACCTTACTTGGCTAATCTCCTTTTGGGTTTGCTTCAGAGAAATCAAAA
  GGATAGAATGGACTTTGAAGCATTTTTCAGCCATCCTTTCCTTGAGCAAGTTCCAGTTAAAAAATCTTGC
  CCAGTCCCAGTGCCTGTGTATTCTGGCCCTGTCCCTGGAAGCTCCTGCAGCAGCTCACCATCTTGTCGCT
  TTGCTTCTCCACCATCCCTTCCAGATATGCAGCATATTCAGGAAGAAAACTTATCCTCCCCACCGTTGGG
  TCCTCCCAACTATCTACAGGTGTCCAAAGACTCTGCGAGTAATAGTAGCAAGAACTCTTCTTGTGACACG
  GATGACTTTGTTTTGGTTCCACACAACATCTCGTCAGACCACTCATATGACATGCCAATGGGGACTACGG
  CCAGACGTGCTTCAAATGAATTCTTTATGTGTGGAGGGCAGTGTCAACCTACTGTGTCACCTCACAGCGA
  AACAGCCCCAATTCCAGTTCCTACTCAAGTAAGGAATTATCAGCGCATAGAACAGAATCTTATATCCACT
  GCCAGCTCTGGCACAAACCCACATGGTTCTCCAAGATCTGCAGTAGTACGAAGGTCTAATACCAGCCCCA
  TGGGCTTCCTCCGGGTTGGGTCCTGCTCCCCTGTACCAGGAGACACAGTGCAGACAGGAGGACGAAGACT
  CTCTACTGGCTCTTCCAGGCCTTACTCACCATCCCCTTTGGTTGGTACCATTCCTGAACAGTTTAGTCAG
  TGCTGCTGTGGACATCCTCAGGGCCATGAAGCCAGGAGTAGGCACTCCTCAGGTTCTCCAGTGCCACAGA
  CCCAGGCACCACAGTCACTCTTACTGGGTGCTAGACTGCAGAGTGCACCCACCCTCACCGATATCTATCA
  GAACAAGCAGAAGCTCAGAAAGCAGCACTCTGACCCTGTGTGTCCGTCCCATGCTGGAGCTGGGTATAGT
  TACTCACCTCAGCCTAGTCGGCCTGGCAGCCTTGGGACCTCTCCCACCAAGCACACGGGGTCCTCTCCAC
  GGAATTCTGACTGGTTCTTTAAAACTCCTTTACCAACAATCATTGGCTCTCCTACTAAGACTACAGCTCC
  TTTCAAAATCCCTAAAACACAAGCATCTTCTAACCTGTTAGCCTTGGTTACTCGTCATGGGCCTGCTGAA
  AGCCAGTCCAAAGATGGGAATGACCCTCGTGAGTGTTCCCACTGCCTCTCAGTACAAGGAAGCGAGAGGC
  ATCGATCTGAGCAGCAGCAGAGCAAGGCAGTGTTTGGCAGATCTGTCAGTACTGGGAAGTTATCAGAACA
  ACAAGTAAAGGCACCTTTAGGTGGACACCAGGGCAGCACGGATAGTTTAAACACAGAACGACCAATGGAT
  GTAGCTCCTGCAGGAGCCTGTGGTGTTATGCTGGCATTGCCAGCAGGAACAGCAGCAAGCGCCAGAGCTG
  TCCTCTTCACCGTGGGGTCTCCTCCACACAGTGCCACAGCCCCCACTTGTACTCATATGGTCCTTCGAAC
  AAGAACCACCTCAGTGGGGTCCAGCAGCTCAGGAGGTTCCTTGTGTTCTGCAAGTGGCCGAGTATGTGTG
  GGCTCCCCTCCTGGACCAGGGTTGGGCTCTTCCCCACCAGGAGCAGAGGGAGCTCCCAGCCTAAGATACG
  TGCCTTATGGTGCTTCACCACCCAGCCTAGAGGGTCTCATCACCTTTGAAGCCCCTGAACTACCAGAGGA
  GACACTGATGGAGCGAGAGCACACAGACACCTTACGCCATCTGAACATGATGTTAATGTTTACTGAGTGT
  GTGCTGGACCTGACGGCAGTGAGGGGTGGGAACCCTGAGCTGTGCACATCTGCTGTGTCCTTGTACCAGA
  TTCAGGAGAGTGTAGTTGTGGACCAGATCAGCCAGCTAAGCAAAGATTGGGGGCGGGTGGAGCAGCTGGT
  GTTGTACATGAAGGCAGCACAGCTGCTGGCGGCTTCCCTGCATCTCGCCAAAGCTCAGGTCAAGTCTGGG
  AAGCTGAGCCCATCCATGGCTGTGAAACAAGTTGTTAAAAATCTGAATGAAAGATACAAATTCTGCATCA
  CCATGTGCAAGAAACTTACAGAAAAGCTGAATCGCTTCTTCTCCGATAAACAGAGATTTATTGATGAAAT
  CAACAGTGTGACTGCAGAGAAACTCATCTATAATTGTGCTGTGGAAATGGTTCAATCTGCAGCCCTGGAT
  GAGATGTTTCAGCAGACTGAAGACATCGTTTATCGCTACCACAAGGCAGCCCTTCTTTTGGAAGGCTTAA
  GTAAGATCCTGCAGGACCCTACAGATGTTGAAAATGTGCATAAGTATAAATGTAGTATTGAAAGAAGATT
  GTCAGCACTCTGCTGTAGCACTGCAACTGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC053029
• Insert Size: 3114 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053029, AAH53029
• RefSeq Size: 4413 bp
• RefSeq ORF: 3113 bp
• Locus ID: 29869
• Cytogenetics: 11 B2
• Gene Summary: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.[UniProtKB/Swiss-Prot Function]