Top3a (NM_009410) Mouse Untagged Clone

CAT#: MC223078

Top3a (untagged) - Mouse topoisomerase (DNA) III alpha (Top3a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Top3a
• Synonyms: MGC106383
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223078 representing NM_009410
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTTCCCGGTCACCCTCTTAGCGTTTCAGTGGCACCGACGGCCTGGAGGCCGTGCCCTGTCCCGCG
  CTGCCATGGAAGTGGCCTTCCGAGGAGTGCGAAAAGTTCTCTGCGTGGCGGAGAAAAACGACGCTGCCAA
  GGGGATCGCCGACTTGCTGTCCAACGGGCGTATGAGGCGGAAAGAAGGCCTTTCTAAATTCAACAAGATT
  TATGAATTTGACTATCATCTGTATGGCCAGAATGTTACTATGATAATGACTTCAGTCTCTGGACATTTGC
  TGGCTCATGACTTCCAGATGCAGTTTCGGAAATGGCAGAGCTGCAATCCCCTTGTCCTCTTTGAAGCAGA
  AATTGAAAAGTATTGCCCAGAAAATTTTATAGACATCAAGAAAACTCTAGAACGAGAGACACATCATTGT
  CAGGCCCTGGTGATCTGGACGGACTGTGATAGGGAAGGTGAGAACATCGGCTTTGAGATTATCCACGTAT
  GCAAGGCTGTAAAACCCAATCTACGGGTGCTGAGAGCCCGCTTCTCTGAGATCACGCCACACGCCGTCAG
  AACAGCCTGTGAAAACCTAACTGAGCCTGACCAGAGAGTGAGTGACGCAGTGGATGTGAGGCAAGAGCTG
  GACCTGAGGATCGGAGCTGCCTTCACAAGGTTCCAGACCCTGCGGCTCCAGAGGATCTTCCCTGAGGTGC
  TGGCAGAGCAGCTCATCAGCTACGGCAGTTGCCAGTTCCCAACACTGGGGTTTGTGGTGGAGAGGTTCAA
  GGCCATTCAGGCTTTTGTGCCAGAAGTCTTCCACAAAATTAAAGTAACTCATGACCACAAAGATGGGACC
  GTAGAGTTCAACTGGAAACGATACCGTCTCTTTAACCACACAGCTTGTCTTGTCCTTTATCAGTTGTGCA
  TGGAGGATCCCATGGCGACTGTGGTAGAGGTCAGATCTAAGCCAAAGAGCAAGTGGAGGCCGCAGGCTTT
  GGACACGGTGGAGCTAGAGAAACTGGCATCTCGGAAATTGAGAATAAATGCCAAAGAAACCATGAGGATC
  GCGGAAAAGCTCTATACACAAGGGTACATCAGCTACCCCCGGACAGAAACAAACATCTTCCCCAAAGACT
  TAAACCTGGTTGCATTGGTAGAACAGCAGACCGTGGATCCACACTGGGGGGCTTTTGCTCAGACCATTCT
  AGAGCGAGGTGGCCCTACTCCCCGAAACGGGAGCAAGTCTGATCAAGCTCACCCTCCCATTCACCCCACC
  AAATACACCAGCGGCTTGCAGGGAGATGATCGGCGACTGTACGAGTTCATTGTTCGCCATTTCCTGGCTT
  GCTGCTCTCAGGACGCTCAGGGGCAAGAGACTACCGTAGAGATCGACATTGCCCAAGAGCGCTTTGTGGC
  CCACGGGCTCATCATTCTGGCCCGTAACTACCTAGATGTGTATCCGTATGATCACTGGAGTGACAAGCTC
  CTCCCCGTCTATGAACAAGGCTCCCACTTCCAGCCCAGCACTGTGGAAATGGTGGATGGAGAGACTAGCC
  CACCCCAACTGCTTACCGAAGCTGACCTCATCGCCCTCATGGAGAAACATGGGATCGGTACTGATGCAAC
  TCATGCAGAACACATTGAGACCATCAAAGCCCGGATGTATGTGGGACTCACCTCAGACAAGCGGTTCCTG
  CCGGGGCACCTGGGCATGGGACTTGTAGAAGGTTACGATTCCATGGGTTATGAGATGTCCAAGCCTGACC
  TCCGTGCCGAGCTGGAAGCTGATCTCAAGCTGATCTGTGAGGGCAAGAAGGATAAGTTTCAAGTTCTAAG
  GCAGCAAGTGCAAAAATACAAGCAGGTTTTCATTGAAGCAGTGGCGAAGGCAAAGAAGTTGGATGAGGCT
  TTATCTCAGTACTTAGGAGAAAGAACAGAGATGGCCCAGCAAGAAGAGATCTACCCAGCCATGCCAGAGC
  CTGTTCGGAAGTGCCCACAGTGTAACAAGGATATGGTCCTCAAGACCAAGAAGAGTGGTGGGTTCTACCT
  CAGCTGCATGGGGTTCCCAGAGTGTCGGTCAGCTGTGTGGTTTCCTGACTCGGTGCTGGAGGCCAGCAGG
  GACAACAGTGTGTGTTCTGTTTGTCAGCCACCCCCTGTGTACAGGTTGAAGTTGAAGTTTAAGCGTGGTA
  GCCTTCCCCCAGCTATGCCCCTGGAGTTTGTCGGCTGCATAGGTGGATGTGATGAGACTTTGAAGGAAAT
  CTTCGGCCTGCGATTTCCACGGGCTCTCCCAAGAGCTAGCCAGCCCTCTGGCCACCTGCAGGCCAGCCAG
  GCCCTCAACAGGATGGACAGCAGTCAGCATAACCTGTCTCAACCTCTGGTTAACAGACATACTAGACCTT
  CAAAGACAGTGGCCCAAGCCTTGCTACCACCCACTACTGCTGGTGAAAGCAACTCTGTGACTTGCAATTG
  TGGCCGGGAGGCTGTGCTGCTCACTGTCCGCAAGCAGGGCCCCAACCAGGGCCGGCATTTCTACAAGTGC
  AGTAATGGTGACTGCAACTTCTTCCTGTGGGCTGACAGCAGCCATTCCACTGGAGGAGGGACCCCCACCT
  CTGCATCAGGACCCCCAGGCAGCTCTGTAGGATGCCCATCCAGTGTAGGCAGCCACATGGATGGGTTTGG
  CAGCCTTGGCAGCGACAGTGATGGAGGTACACCCTGCCTGTGCGGGCAGCCTGCTGTCACACGGACTGTT
  CAGAAGGATGGACCCAACAAAGGACGCCAGTTCCACACCTGTGCCAAGCCACGAGAGCAGCAGTGTGGCT
  TCTTTCAGTGGGTGGATGAGAATGTGGCCCCAGGAAGTTTTGCAGCCCCTGCATGGCCAGGAGGCAGAGG
  AAAAGCCCAGAGACCAGAGGCTGCAAGCAAAAGACCAAGAGCTGGTTCCTCAGATGCAGGGTCCACAGTA
  AAAAAGCCTCGGAAATGTAGCCTTTGTCACCAGCCTGGACACACCCGAACCTTTTGTCCTCAGAACAGAT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009410
• Insert Size: 3012 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009410.2, NP_033436.1
• RefSeq Size: 3740 bp
• RefSeq ORF: 3012 bp
• Locus ID: 21975
• UniProt ID: O70157
• Cytogenetics: 11 B2
• Gene Summary: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2.[UniProtKB/Swiss-Prot Function]