Timeless (BC058641) Mouse Untagged Clone

CAT#: MC223076

Timeless (untagged) - Mouse timeless homolog (Drosophila) (cDNA clone MGC:73470 IMAGE:6837105), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Timeless
• Synonyms: tim, Debt69
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058641
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTGTACATGATGAACTGTGAACTTCTAGCCACGTGTAGCGCCCTTGGGTACTTGGAAGGAGGGA
  CTTACCACAAGGAGCCGGATTGCCTGGAGAGTGTGAAGGATTTGATCCGATACCTGAGGCACGAGGATGA
  GACCCGAGATGTGCGGCAGCAGCTGGGAGCTGCACAGATCCTGCAGAGCGACCTCCTGCCAATCCTCACG
  CAGCATCGCCAGGACAAGCCTCTCTTCGATGCCGTGATCAGGCTGATGGTAAATTTGACACAGCCAGCCT
  TGCTCTGTTTTGGCAGCGTGCCTAAGGACTCCAGTGTACGGCACCATTTTCTGCAGGTTCTAACGTACCT
  GCAAGCCTACAAAGAGGCCTTTGCCAGTGAGAAGGCATTTGGAGTCCTCAGCGAGACCTTGTATGAATTG
  CTACAGCTGGGCTGGGAGGATCGGCAAGAAGAAGACAACTTGCTGATCGAGCGGATCCTTCTGCTGGTCA
  GAAATATTCTCCATGTCCCGGCCAACCTTGAGCAGGAGAAGAGTATCGATGATGATGCCAGCATCCACGA
  CCGTCTCCTTTGGGCAATTCACCTCAGTGGCATGGACGACTTGCTCCTCTTCCTGTCCAGCTCATCCGCC
  GAGCAGCAGTGGAGCCTCCATGTGCTGGAGATCATCTCCCTCATGTTCCGAGACCAGACCCCTGAGCAGC
  TAGCGGGAGTAGGGCAGGGACGCTTGGCTCAGGAGCGAAGCACGGATGTGGCAGAATTGGAGGTGCTGCG
  CCAACGGGAGATGGCGGAGAAGAGAGCTCGGGCCCTCCAGCGAGGAAACAGGCACTCTCGATTTGGGGGC
  TCCTACATTGTCCAGGGGTTGAAATCTATTGGGGAGAAGGATGTCGTCTTTCACAAAGGCCTTCACAATC
  TCCAGAACTACAGCTCAGATCTGGGAAAGCAGCCCAGGAGGGTGCCCAAGCGTCGTCAGGCTGCCCAGGA
  GCTGTCTGTCCATCGCCGCTCTGTCCTGAATGTGAGACTCTTCCTCAGAGACTTCTGCTCTGAGTTCCTG
  GAGAACTGCTACAACCCGCTCATGGGCGCGGTCAAGGATCATCTGCTTCGGGAGAGAGCGCAGCAGCATG
  ACGAGACTTACTACATGTGGGCAATGGCTTTCTTCATGGCCTTCAACCGAGCTGCCACCTTCCGCCCCGG
  CCTTGTTTCTGAGACCCTCAGTATCCGTACCTTTCACTTTGTGGAGCAGAACCTCACCAACTACTACGAG
  ATGATGCTGACAGACCGCAAGGAGGCCGCCTCCTGGGCGCGCAGGATGCACCTGGCCCTGAAGGCCTACC
  AGGAGCTGCTGGCCACGGTGAACGAGATGGACATGTGCCCAGATGAGGCTGTTAGGGAGAGCAGTCGTAT
  CATCAAAAACAACATTTTCTATATGATGGAGTACCGAGAACTATTCCTGGCGCTCTTTCGAAAGTTTGAT
  GAGAGATACCATCCACGCTCATTCCTTCGAGACCTGGTGGAAACCACCCACCTCTTCCTCAAAATGTTGG
  AGCGCTTTTGCCGGAGCCGCGGGAACCTGATGGTGCAGAACAAAAGAAAAAAGAGGAAAAAGAAAAAGAA
  GGTTCAGGACCAGGGTGTTGCTTTCTCACAAAGCCCCGGGGAGCTGGAGGCCATGTGGCCAGCCCTGGCA
  GAGCAGCTGCTGCAGTGTGCCCAGGACCCTGAGCTCAGTGTGGACCCCGTCGTTCCCTTTGATGCGGCCT
  CAGAGGTGCCAGTGGAGGAGCAGCGGGTAGAAGCCATGGTGAGGATCCAAGACTGCCTTACGGCTGGCCA
  GGCCCCGCAAGCCCTGGCCCTCCTGCGGTCTGCCCGGGAAGTGTGGCCTGAAGGAAATGCGTTTGGCTCT
  CCAGTCATTTCCCCAGGGGAAGAAATGCAGTTGCTGAAACAAATCCTCTCCACGCCCCTTCCCCGGCAGC
  AGGAGCCAGAAGAAGGAGATGCAGAGGAGGAAGAGGAAGAGGAGGAGGAAGAGGAGTTACAGGTGGTCCA
  GGTGTCAGAGAAGGAGTTTAACTTTCTGGAATACCTGAAACGCTTCGCATCCTCAACCATCGTGCGGGCC
  TACGTGCTTCTCCTGCGGAGCTACAGGCAGAACAGTGCTCACACCAACCACTGCATCGCCAAGATGCTGC
  ACCGGCTGGCCCATGGCCTGGGGATGGAAGCCCTGCTTTTCCAGCTGTCCCTGTTCTGCCTCTTCAATCG
  GCTGCTTAGTGACCCAGCTGCTGCGGCCTACAAAGAGCTAGTGACTTTTGCCAAATACATCATTGGCAAG
  TTCTTTGCGTTGGCTGCCGTGAACCAGAAAGCGTTTGTGGAGCTGCTGTTCTGGAAGAACACCGCAGTGG
  TTCGGGAAATGACCCAGGGATATGGCTCCCTCGACAGTGGGTCTTCCAGCCACAGAGCTCCTCTGTGGAG
  CCCTGAGGAAGAGGCCCAGCTTCAGGAACTATACCTCGCCCACAAGGATGTGGAAGGTCAAGATGTAGTG
  GAAACCATATTGGCGCACCTGAAAGTCGTTCCTCGAACACGCAAGCAGGTCATCCACCACCTGGTCCGGA
  TGGGCCTGGCCGACAGCGTCAAGGAGTTCCAGAAGAGGAAAGGGACCCAGATTGTCTTGTGGACGGAGGA
  CCAGGAGCTGGAGTTACAGCGGCTCTTTGAGGAGTTCCGGGACTCTGATGATGTTCTTGGTCAAATCATG
  AAGAATATCACAGCCAAACGTTCACGGGCTCGAGTAGTGGACAAACTGTTGGCCCTGGGGTTGGTGTCTG
  AGCGGAGGCAGCTATACAAGAAACGGAGAAAGAAGCTGGCGCCTTCTTGCATGAATGGAGAAAAGTCCCC
  GAGAGACCCCTGGCAGGAAGATCCGGAAGAGGAGCCAGAGCCAGGAGTCCCCGGAGAGCAGGGTCCCAGT
  GAGGAGCACCGGACAGAAGCCCTGAGAGCCCTTCTGTCAGCCCGTAAGAGGAAAGCAGGCCTGGGGCCTA
  CAGAAGAGGAGGCCACTGGGGAGGAAGAATGGAACTCAGCGCCCAAGAAGCGGCAACTGCTGGACAGCGA
  CGAAGAGGAAGATGATGAGGGGAGGAGGCAAGCAGTGTCGGGAACGCCAAGAGTCCACAGGAAGAAACGG
  TTTCAGATTGAGGATGAGGATGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC058641
• Insert Size: 3177 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058641, AAH58641
• RefSeq Size: 4002 bp
• RefSeq ORF: 3176 bp
• Locus ID: 21853
• Cytogenetics: 10 76.49 cM
• Gene Summary: The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]