Tek (BC050824) Mouse Untagged Clone

CAT#: MC223075

Tek (untagged) - Mouse endothelial-specific receptor tyrosine kinase (cDNA clone MGC:59299 IMAGE:6333576), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tek
• Synonyms: AA517024; Cd202b; Hyk; STK1; Tie-2; Tie2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050824
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCTTTAGCCGGCTTAGTTCTCTGTGGAGTCAGCTTGCTCCTTTATGGAGTAGTAGAAGGCGCCA
  TGGACCTGATCTTGATCAATTCCCTACCTCTTGTGTCTGATGCCGAAACATCCCTCACCTGCATTGCCTC
  TGGGTGGCACCCCCATGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTTAATGAACCAGCACCAAGAT
  CCACTGGAGGTTACTCAAGATGTGACCAGAGAATGGGCGAAAAAAGTTGTTTGGAAGAGAGAAAAGGCCA
  GTAAGATTAATGGTGCTTATTTCTGTGAAGGTCGAGTTCGAGGACAGGCTATAAGGATACGGACCATGAA
  GATGCGTCAACAAGCATCCTTCCTACCTGCTACTTTAACTATGACCGTGGACAGGGGAGATAATGTGAAC
  ATATCTTTCAAAAAGGTGTTAATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGATGTGAAGCTCAGA
  AGTGGGGGCCCGACTGTAGCCGTCCTTGTACTACTTGCAAGAACAATGGAGTCTGCCATGAAGATACCGG
  GGAATGCATTTGCCCTCCTGGGTTTATGGGGAGAACATGTGAGAAAGCTTGTGAGCCGCACACATTTGGC
  AGGACCTGTAAAGAAAGGTGTAGTGGACCAGAAGGATGCAAGTCTTATGTGTTCTGTCTCCCAGACCCTT
  ACGGGTGTTCCTGTGCCACAGGCTGGAGGGGGTTGCAGTGCAATGAAGCATGCCCATCTGGTTACTACGG
  ACCAGACTGTAAGCTCAGGTGCCACTGTACCAATGAAGAGATATGTGATCGGTTCCAAGGATGCCTCTGC
  TCTCAAGGATGGCAAGGGCTGCAGTGTGAGAAAGAAGGCAGGCCAAGGATGACTCCACAGATAGAGGATT
  TGCCAGATCACATTGAAGTAAACAGTGGAAAATTTAACCCCATCTGCAAAGCCTCTGGGTGGCCACTACC
  TACTAGTGAAGAAATGACCCTAGTGAAGCCAGATGGGACAGTGCTCCAACCAAATGACTTCAACTATACA
  GATCGTTTCTCAGTGGCCATATTCACTGTCAACCGAGTCTTACCTCCTGACTCAGGAGTCTGGGTCTGCA
  GTGTGAACACAGTGGCTGGGATGGTGGAAAAGCCTTTCAACATTTCCGTCAAAGTTCTTCCAGAGCCCCT
  GCACGCCCCAAATGTGATTGACACTGGACATAACTTTGCTATCATCAATATCAGCTCTGAGCCTTACTTT
  GGGGATGGACCCATCAAATCCAAGAAGCTTTTCTATAAACCTGTCAATCAGGCCTGGAAATACATTGAAG
  TGACGAATGAGATTTTCACTCTCAACTACTTGGAGCCGCGGACTGACTACGAGCTGTGTGTGCAGCTGGC
  CCGTCCTGGAGAGGGTGGAGAAGGGCATCCTGGGCCTGTGAGACGATTTACAACAGCGTCTATCGGACTC
  CCTCCTCCAAGAGGTCTCAGTCTCCTGCCAAAAAGCCAGACAGCTCTAAATTTGACTTGGCAACCGATAT
  TTACAAACTCAGAAGATGAATTTTATGTGGAAGTCGAGAGGCGATCCCTGCAAACAACAAGTGATCAGCA
  GAACATCAAAGTGCCTGGGAACCTGACCTCGGTGCTACTGAGCAACTTAGTCCCCAGGGAGCAGTACACA
  GTCCGAGCTAGAGTCAACACCAAGGCGCAGGGGGAGTGGAGTGAAGAACTCAGGGCCTGGACCCTTAGTG
  ACATTCTCCCTCCTCAACCAGAAAACATCAAGATCTCCAACATCACTGACTCCACAGCTATGGTTTCTTG
  GACAATAGTGGATGGCTATTCGATTTCTTCCATCATCATCCGGTATAAGGTTCAGGGCAAAAATGAAGAC
  CAGCACATTGATGTGAAGATCAAGAATGCTACCGTTACTCAGTACCAGCTCAAGGGCCTAGAGCCAGAGA
  CTACATACCATGTGGATATTTTTGCTGAGAACAACATAGGATCAAGCAACCCAGCCTTTTCTCATGAACT
  GAGGACGCTTCCACATTCCCCAGCCTCTGCAGACCTCGGAGGGGGAAAGATGCTACTCATAGCCATCCTT
  GGGTCGGCTGGAATGACTTGCATCACCGTGCTGTTGGCGTTTCTGATTATGTTGCAACTGAAGAGAGCAA
  ATGTCCAAAGGAGAATGGCTCAGGCATTCCAGAACGTGAGAGAAGAACCAGCTGTGCAGTTTAACTCAGG
  AACTCTGGCCCTTAACAGGAAGGCCAAAAACAATCCGGATCCCACAATTTATCCTGTGCTTGACTGGAAT
  GACATCAAGTTTCAAGACGTGATCGGAGAGGGCAACTTTGGCCAGGTTCTGAAGGCACGCATCAAGAAGG
  ATGGGTTACGGATGGATGCCGCCATCAAGAGGATGAAAGAGTATGCCTCCAAAGATGATCACAGGGACTT
  CGCAGGAGAACTGGAGGTTCTTTGTAAACTTGGACACCATCCAAACATCATTAATCTCTTGGGAGCATGT
  GAACACCGAGGCTATTTGTACCTAGCTATTGAGTATGCCCCGCATGGAAACCTCCTGGACTTCCTGCGTA
  AGAGCAGAGTGCTAGAGACAGACCCTGCTTTTGCCATCGCCAACAGTACAGCTTCCACACTGTCCTCCCA
  ACAGCTTCTTCATTTTGCTGCAGATGTGGCCCGGGGGATGGACTACTTGAGCCAGAAACAGTTTATCCAC
  AGGGACCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTACATAGCCAAAATAGCAGATTTTGGATTGT
  CACGAGGTCAAGAAGTGTATGTGAAAAAGACAATGGGAAGGCTCCCAGTGCGTTGGATGGCAATCGAATC
  ACTGAACTATAGTGTCTATACAACCAACAGTGATGTCTGGTCCTATGGTGTATTGCTCTGGGAGATTGTT
  AGCTTAGGAGGCACCCCCTACTGCGGCATGACGTGCGCGGAGCTCTATGAGAAGCTACCCCAGGGCTACA
  GGCTGGAGAAGCCCCTGAACTGTGATGATGAGGTGTATGATCTAATGAGACAGTGCTGGAGGGAGAAGCC
  TTATGAGAGACCATCATTTGCCCAGATATTGGTGTCCTTAAACAGGATGCTGGAAGAACGGAAGACATAC
  GTGAACACCACACTGTATGAGAAGTTTACCTATGCAGGAATTGACTGCTCTGCGGAAGAAGCAGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC050824
• Insert Size: 3219 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050824, AAH50824
• RefSeq Size: 4549 bp
• RefSeq ORF: 3218 bp
• Locus ID: 21687
• Cytogenetics: 4 43.34 cM
• Gene Summary: Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1 and TIE1.[UniProtKB/Swiss-Prot Function]