Heph (NM_001159627) Mouse Untagged Clone

CAT#: MC223056

Heph (untagged) - Mouse hephaestin (Heph), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Heph
• Synonyms: C130006F04Rik; Cpl; mKIAA0698; sla
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223056 representing NM_001159627
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCAGGCCATCTTCTCTGGGCTTTACTGTTGATGCACTCCTTGTGGTCTATACCAACTGATGGGG
  CCATTCGAAACTACTACTTGGGCATCCAGGATATGCAGTGGAACTATGCTCCCAAAGGAAGAAATGTCAT
  CACAAATCAGACTCTCAACAATGACACAGTGGCTTCCAGTTTCCTGAAGTCTGGCAAAAACAGGATAGGG
  AGTAGTTACAAGAAGACTGTTTATAAGGAATACAGTGATGGCACATACACTGAAGAAATAGCCAAGCCTG
  CCTGGTTGGGCTTCCTAGGACCACTGTTACAGGCTGAGGTGGGGGATGTCATCTTGATTCACCTGAAGAA
  TTTTGCGAGCCGACCTTACACCATTCACCCTCACGGTGTTTTTTATGAGAAGGACTCAGAAGGCTCACTA
  TACCCAGATGGTTCTTCTGGGTATCTGAAAGCGGATGATTCTGTCCCCCCTGGGGGCAGCCATGTCTACA
  ACTGGAGTATTCCAGAAAGTCATGCCCCCACTGAGGCAGACCCAGCATGCCTCACCTGGATTTACCACTC
  GCATGTAGATGCTCCAAGAGACATTGCAACTGGTCTCATTGGACCTCTTATCACCTGTAAAAGAGGGACC
  CTGGATGGTAATTCCCCACCTCAGAGGAAGGATGTGGACCATAATTTCTTCCTCCTCTTCAGTGTGATAG
  ATGAGAACCTTAGCTGGCACCTTGATGACAACATTGCTACTTACTGCTCAGACCCTGCCTCGGTGGACAA
  AGAAGATGGAGCCTTTCAAGACAGCAACAGGATGCATGCAATCAATGGGTTTGTCTTTGGGAACTTACCA
  GAGTTGAGCATGTGTGCACAGAAGCATGTGGCCTGGCACTTGTTTGGCATGGGCAATGAAATAGATGTCC
  ACACAGCTTTCTTCCATGGACAGATGCTGAGTATCCGTGGACACCACACTGATGTTGCAAACATTTTTCC
  AGCTACCTTTGTGACTGCTGAGATGGTGCCCCAAAAGTCTGGAACCTGGCTAATTAGCTGTGAAGTGAAC
  AGCCACTTGAGAAGTGGCATGCAGGCCTTCTACAAGGTTGACTCTTGCTCCATGGACCCACCTGTGGACC
  AGCTCACTGGCAAAGTTCGTCAGTACTTCATTCAGGCCCATGAGATTCAATGGGACTATGGTCCAATAGG
  GTATGATGGCAGAACTGGGAAGAGTTTGAGAGAGCCAGGAAGTGGCCCAGATAAGTACTTCCAGAAGAGC
  TCTAGTCGAATTGGAGGTACTTACTGGAAAGTTCGATATGAAGCCTTTCAAGATGAGACATTCCAGGAAA
  GGGTACATCAGGAAGAAGAAACACATCTTGGAATACTGGGACCAGTGATAAGGGCTGAAGTGGGTGACAC
  CATCCAGGTGGTCTTCTATAACCGTGCCTCCCAGCCATTCAGCATACAGCCCCATGGTGTCTTTTATGAG
  AAAAACTCTGAGGGCACCGTGTACAATGATGGCACATCTCATCCCAAAGTAGCCAAGTCATTTGAAAAAG
  TCACATACTACTGGACGGTTCCTCCCCATGCTGGGCCCACTGCTCAGGATCCTGCCTGTCTAACCTGGAT
  GTACTTCTCTGCTGCAGATCCCACAAGAGATACAAATTCTGGCCTGGTGGGCCCTCTACTGGTGTGCAAG
  GCTGGGGCCTTGGGTGCAGATGGCAAGCAGAAAGGAGTGGATAAAGAATTTTTTCTCCTCTTCACTGTGT
  TTGATGAGAATGAGAGCTGGTACAACAATGCCAATCAGGCAGCTGGTATGTTGGATTCCCGACTGCTCTC
  AGAGGATGTCGAGGGCTTCCAGGACTCCAATCGAATGCATGCTATTAATGGATTTCTGTTCTCTAACCTG
  CCCAGGCTGGACATGTGCAAGGGTGATACTGTGGCCTGGCACCTGCTTGGCCTGGGCACAGAGACTGATG
  TACATGGGGTAATGTTCGAGGGCAACACTGTGCAGCTTCAGGGCATGAGGAAAGGTGCAGTCATGCTCTT
  TCCTCACACCTTTGTGACGGCCATCATGCAGCCTGACAATCCTGGAATATTTGAAATCTACTGCCAAGCA
  GGCAGCCACCGAGAGGAAGGGATGCAGGCAATTTATAATGTCTCTCAGTGTTCTAGTCATCAAGACAGCC
  CACGCCAACACTACCAAGCTTCAAGAGTCTACTATATCATGGCAGAAGAGATAGAGTGGGATTACTGCCC
  TGATAGAAGCTGGGAACTGGAATGGCATAACACATCTGAGAAAGACAGCTATGGCCATGTTTTCCTGAGC
  AATAAAGATGGGCTCCTGGGTTCCAAATATAAGAAAGCTGTATTCAGGGAATACACTGATGGTACTTTCA
  GAATACCTCGGCCAAGGTCTGGACCAGAGGAGCACTTGGGAATCCTGGGTCCACTTATCAGAGGAGAGGT
  TGGTGATATCTTGACTGTAGTGTTCAAGAATAAGGCCAGTCGACCATATTCTATACATGCCCATGGAGTT
  CTAGAATCTAACACTGGCGGGCCACAGGCTGCTGAGCCTGGTGAAGTACTTACTTACCAGTGGAACATCC
  CAGAAAGATCTGGTCCTGGTCCTAGTGACTCTGCTTGTGTTTCCTGGATTTATTATTCTGCAGTGGATCC
  CATCAAGGACATGTATAGTGGTCTGGTTGGACCCCTAGTCATCTGCAGAAATGGTATCTTGGAACCCAAT
  GGAGGCCGGAATGATATGGACCGGGAATTTGCCTTGTTGTTTTTGATCTTTGATGAGAACCAATCTTGGT
  ATCTGAAGGAGAATATTGCAACATATGGACCTCAAGAATCAAGTCATGTTAACTTGAAGGATGCCACCTT
  CCTAGAGAGCAATAAAATGCATGCTATCAATGGGAAACTCTATGCAAACCTCAGGGGTCTTACTGTATAC
  CAAGGAGAACGAGTAGCCTGGTACATGCTAGCCATGGGCCAAGATACTGACATTCACACTGTACACTTCC
  ATGCAGAGAGTTTCCTCTATCAGAATGGGCAAAGTTACAGGGCAGATGTGGTGGATCTCTTCCCAGGAAC
  ATTTGAAGTTGTGGAGATGGTAGCCAGCAACCCTGGGACATGGCTGATGCACTGCCATGTGACTGACCAT
  GTTCATGCTGGCATGGAGACCATCTTTACGGTCTTGTCTCATGAAGAACATTTCAGCACTATGACCACTA
  TTACTAAAGAGATTGGAAAAGCAGTGATTCTAAGGGACATTGGAGGTGACAATGTGAAGATGCTGGGCAT
  GAACATTCCCATAAAGGATGTAGAGATTCTGTCTTCTGCTTTGATTGCCATATGTGTGCTTCTGTTGCTC
  ATTGCTCTGGCTCTTGGTGGTGTAGTCTGGTACCAGCATCGACAAAGAAAGCTTCGGCGCAACAGGAGGT
  CCATTCTTGATGATAGCTTCAAGCTTCTCTCTCTCAAGCAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159627
• Insert Size: 3474 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159627.1, NP_001153099.1
• RefSeq Size: 4685 bp
• RefSeq ORF: 3474 bp
• Locus ID: 15203
• UniProt ID: Q9Z0Z4
• Cytogenetics: X 42.69 cM
• Gene Summary: May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) represents use of an alternate promoter, compared to variant 1, and enocdes the longest isoform (1). Both variants 1 and 3 encode the same isoform.