Ccdc40 (BC085159) Mouse Untagged Clone

CAT#: MC223030

Ccdc40 (untagged) - Mouse coiled-coil domain containing 40 (cDNA clone MGC:109697 IMAGE:30533905), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc40
• Synonyms: B930008I02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC085159
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGATGCTGAGAAAGTCAGCACGGATGGGGAAGCCATCTCTGAAGGAGAAGTGGGGTCCAATGGAG
  AGACTCCCCCAGAAACAGAAGTGGAATTCATTGGAGAGACCGCTCCAGACACAGATGTGGAATTCATTGG
  AGAGACCTCCCCAGGAACAGACGTGGAACCCACTGGAGAGAGCATACAGGAAACAGAAGTTGAATCCATC
  GGAGAGGCTACCCCAGGAATGGACGTGGAACCCATTAAAAAGACCATGACAAAACTCAACGTTGAATCCA
  TCGGAGAAGAAACCTCAGAAACAGACGTGGACTCCATTAGGAAGGCCCTCCGAGGAATAGACTTGGAATC
  TATCACAGTTGCCTATCCGCCAAAAAAGGCTAAACACAGAAAGGTCAGACCCCAAGCGGAAGTTGAATCC
  ACTGGAAGAGCTGCCCCAGAAGGAGAATTAGAAGTTTCTGACCACGAGAAAGTAGAAGCCCTTCTCGATG
  AGTTGGATGAGCTCTCTGAGATAGTCAGCTCTCCCGAGGTCTCCTACTCTGACATCAGTCCCTTGGAGAT
  GGGTGAAGATGACACAAACGTCTCAGCCACATCAACCGACACATTCCAGCAAGGCATATATGAGCCCATA
  GAACCCATAGAGCCCACAGAGCCCCCAGAGCCTGCAGAGCCCCCAAAGCCTGCAGAGACCCCAGAGGACA
  GCACGGTGAGAGCCCCAGCGCATCCCTATCAAAGAGACTTCCCAATGGGGGCACGACACCGATTCAGACT
  GAGCATCATGGGAAGTCTGACTCCCTCGGACACGGACGATCTTCCCCTGGAAACGGACGAGCCGCCTCAG
  CAAGAGTCAGTGCAGTCAACTCCCAGAGCCCTTGAAGAAACCCGGATACAGTTCCTAGACCAGGTCCAGA
  GCCTCAGCCCGGAGGCCCTGCTCGATAGAGCAACTGAAGGGAGTGATGAAGCAGAGGAGGAAGGCTCCCA
  GCTGATCGTGCTGGACCCAGACCATCCTCTGATGATCAGATTCCAGGAGGCTCTGAAGGGCTACCTCAAC
  CGGCAGATGGACAAACTGAAGCTGGACGTCCAAGAGCTGGATGTGGCCACCAAGCAAACCCGAAGCCAGA
  GGCAGGAGCTGGGCGTGAACCTCTACGGGGTCCAGCAGCACCTGGCCCGCCTGCAGATGCAGCTGGAGAA
  GAGCCATGACCGGCACTCTCTGGTGGCCTGTGAGCGGAGAAGGAAGGAGGAGGAGCTGCAATGCGCACGC
  TCCGTCTACAACAAGACCTGCCAGACCGCCAATGAGGAACGAAAGAAGTTGGCCGCCCTGCAGACAGAGG
  TGGAGAGCCTGGCCCTGCACCTCTTCTACATGCAGAACATAGAGCAGGACGTGCGCGATGACATCCAAGT
  CATGAAGCAGGTGGTGAGGAAGACCGAGACGGAGAAGATGCACGCCGAGGTGGAGAAGAAAAAGCAGGAC
  CTGTTTGTGGACCAGCTCACCGAAAGGTCACACCAGCTAGAAGAAAACATCGCCCTGTTTGAGGCTCAAT
  ACTTGTCCCAGGCTGAGGACACCCGGGTCCTCAAGAAAGCAGTGACTGAGGCCATCACAGAGATTGACAC
  CATTGCCGTGGAGAAGAAGCGAATCCTACAGCAGTGGACCACCAGCCTAGTGGGCATGAAGCACCGTAAC
  GAGGCATACAAGACAGTGATGGATGCGCTCAGGGAGTGTCAGCATCAGGTGAAGTCCACCGACAGTGAGA
  TCGAAGTCTGCAAGAAGTCCATCATGCAGGAGGAAGAGAAGAATGAGAAGCTGGCCCGTCTCCTGAACCG
  CGCAGAGACAGAAGCCACACTGGTGCAGAAAATGACCGCCCAGTGCTTAAGCAAGCAAGAGGCTCTGCAG
  ACGGAGTTTAACACGTACCAGCTCGCCCTGCAGGACACGGAGGAGATGCTGAACAAGGGCTATGTGGAAC
  ACTCGGCAGTCTTGAGCGAGCTCCAGGCCACACGCCAGGCTTTCCACCAGGAACAGGAGCTACGGCAAAA
  GATGGACATGTCCATGGTGGACAAGCTGCAGGAACAGGGGACCTCCAGCAAGATGACCAAGTACTTTCAT
  CAGCTCCTTCGGAAGCTGCAAAAGGAAAACACCAACCTGGTGACACATCTTTCAAAAATTGACGGCGATA
  TCGCCCAGGCCACCCTGGACATCACCAACACCAACTGCAAAATAGACATGCATAAGAAGAAGCTGACAGA
  AGAGTACAACACGGGAGTGGCTGAGGCGCAGATGACCTGGTTGCGCCTGCAGCAGGAACTGGTCCAGGTC
  ACGCATGAGCGCGAGGAGCAGCTGGTGTCCGTGGACCAACTGAAGAAGGAGGTGCACATCATGGAACAAA
  AGAAGCTTCGCATAGAGAGCAAGATTGCACATGAGAAGAAGGAACAAAAGATAGTGAGCCGCCACATGAG
  GGACCTGGACAACGACCTGAGCAAGCTCAACATGCTGTTGGACAAGAACCGATGCAGTTCGGAAGAGCTG
  GAGCAGAACAATATAGCAACTGAGACGGAGTTTCTGCGCACGCTGAAGGACTCAGAAAGAGAGACCATTC
  AAATGCAGGAAAAGCTGATGGAACTCGCTGAGGAAAAGGCCACCCTCCTCAACAGCTTCATGGAGGCAGA
  GCACCAAATCATGCTGTGGGAGAAGAAAATCCAACTGGCCAAGGAGATGCGCAGCTCGGTGGATTCTGAG
  ACCGGGCAGACAGAGATCCGTGCTATGAAGGCCGAGATCCACAGGATGAAGGTTAGGCACGGGCAGCTAC
  TGAAGCAGCAGGAGAAGATGATCCGTGACATGGAGTTGGCTGTGGCCCGCAGGGAGACCATCGTCGTCCA
  GGCTGAAGGGCAGAGCAAGATAGACAAGAAAGTAATCACCAAGACAGAGTTCCACTACCAGCAGCGCGAG
  CTGCAGAAGAAAGTCAGGGAGATGCACAAGGCCACAGATGACTGCACCAACACCATCTCAGAACTGGAAG
  AAACTCAAAAGTTCCTAAGCAGCTCCCTCCAGGAGAAGCAGCAACTGCTGTCAGAAATGCAGGCTACCAC
  AGATGTTCTTGAAGAGGAGATCAACCAGCTAACAGCTCTCAAACGGCAGAACCTTCTGGAGATCGTGACC
  CTGCAGACCCGTGGCAAGCACCTGCAGGCCGCCATTGAGGGGAAATATGTGTTCCTGCACCGCAACAGCA
  GGTCCCAGCTGATGGAGCGTAAGCGCCTGAGCGTCCGGCTGTCCCAGCTCAACAAGGTCCTCTCCAGCGT
  GCAGGAAGACTACCCCCAGTACCAGGAGGTGCTGCAGAGCATTCAACAGAAGATCGCCACCAAGCTGGAG
  ACCCCAGAGCCCTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC085159
• Insert Size: 3378 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC085159, AAH85159
• RefSeq Size: 4591 bp
• RefSeq ORF: 3377 bp
• Locus ID: 207607
• Cytogenetics: 11 E2
• Gene Summary: Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Probably acts together with CCDC39 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella. Not required for outer dynein arm complexes assembly. Required for axonemal recruitment of CCDC39.[UniProtKB/Swiss-Prot Function]