Phc3 (NM_001165954) Mouse Untagged Clone

CAT#: MC223013

Phc3 (untagged) - Mouse polyhomeotic-like 3 (Drosophila) (Phc3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phc3
• Synonyms: E030046K01Rik; Edr3; Hph3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223013 representing NM_001165954
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGCGGAGTTTAAGGACCACAGCACAGCTATGGATAGTGAGCCGAGCTCTGGAACATCCGTGT
  CAACAACAGCCAGCAGTACCACCACCACCACCATCACCACATCCTCCTCCCGAATGCAGCAGCCACAGAT
  CTCTGTCTACAGCGGGTCAGACCGACATGCTGTTCAGGTAATTCAGCAGGCCTTGCATCGGCCCCCCAGC
  TCGGCAGCTCAGTACCTGCAGCAGATGTACGCGGCTCAGCAGCAGCACCTGATGCTGCACACGGCTGCTC
  TGCAGCAGCAGCACCTAAGCAGCTCGCAGCTCCAGAGCCTTGCGGCTGTCCAGGCAAGTTTGTCCAGTGG
  AAGACCATCTACATCCCCCACAGGCAGTGTTACACAACAGTCAAGTATGTCCCAGACATCTATCCTGTCC
  GCTTCTCCTGCACCTGCTCAGCTGATGAACCGCTCCCAGACGTCCAGTTCTACCAGCGGCAGTATTACCC
  AGCAGACTATGCTACTCGGGAGTACCTCTCCCACCCTAACTGCCAGCCAAGCCCAGATGTATCTCCGAGC
  TCAGATGCTGATTTTCACACCTGCTACCACTGTGGCTGCTGTACAGTCTGACATCCCTGTTGTCTCGTCG
  TCACCGTCACCTTCCTGTCAGTCTGCAGCTGCTCAGGTTCAGAATTTAACCTTACGCAGCCAGAAGCTGG
  GTGTCTTATCTAGCTCACAGAATGGCTCACCAAAAAGCGCTGGTCAAACCCAGTCCTTGACAATTTGCCA
  TAATAAAACAACAGTGACCAGTTCTAAAATCAGCCAACGAGACCCTTCTCCAGAGAGCAAGAAGGGAGGA
  AGCCCAGGTCTGGAATCTCGGAGCACGGCCGTCACTCGGACATCAAGCATCCACCAGTTAATAGCACCAG
  CTTCATATTCTCCAATTCAGCCTCATTCTCTAATCAAACATCAGCAGATTCCTCTTCATTCACCACCTCC
  TAAAGTTTCCCATCATCAGCTGCTATTACAACAGCAACAACAGCAAATTCAGCCAATCACCCTTCAGAGT
  CCAAGTCAAGATCCACCCCCATCCCAGCACTGTATCCCACTCCCAAACCATGGCCTTTCTCCGGCTCCCA
  GTAATGCCCAGCCGCAGCATTGCTCCCCAGTTCAGAGCCATCCTCCGCCTTTAACTGTGTCTCCCAACCA
  GGCACAGTCCGCACAGCAGTCTGTAGTGGTGTCTCCTCCCCCCCCTCATTCCCCAAGTCAGTCTCCTACT
  ATAATTATCCATCCACAAGCACTTATCCAGCCACACCCTCTTGTGTCTTCAGCTCTTCAAACAGGGCCAA
  ATTTGCAGCAGGCTGCTGCTGATCAGGTACAGTCCACCGCACAGCTGAATCTTCCATCCCATCTTCCACT
  TCCAGCATCCCCTGTTGTACACATTGGCCCTGTCCAGCAGTCTGCCTTGGTGTCCCCTGGTCAGCAGATG
  GTGTCCCCAACATCACACCAGCAGTATTCAGCCCTACAGTCCTCTCCAATTCCCATCGCGACCCCTCCAC
  AGATGTCGGCATCTCCTCCAGCTCAGCTCCCACCACTGCCCTTGCAGTCTATGCAGTCTTTACAAGTGCA
  GCCTGAAATTCTATCCCAGGGCCAGGTTTTGGTGCAGAATGCTTTGGTGTCAGAAGAGGAGCTACCTGCT
  GCAGAAGCTTTGGTCCAGTTGCCATTTCAGACTCTTCCTCCTCCACAGACTGTTGCGGTAAATCTACAAG
  TACAGCCGCCAGCACCTGTTGATCCACCAGTGGTTTATCAAGTAGAAGATGTGTGTGAGGAAGAAATGCC
  AGAGGAGTCCGATGAGTGTGCAAGGATGGACAGAACCCCCCCACCCCCCACCTTGTCTCCAGCAGCTGTC
  ACTGTGGGGAGGGGAGAGGACTTGACATCCGAACATCCTTTGTTAGAGCAAGTGGAGTTGCCTGCTGTGG
  CCTCAGTCAGTGCTTCCGTGATCAAGTCTCCATCAGACCCCACCCATGCCTCTGCTCCTGCACCTCCCCT
  CTTAATCCCTGCTGCCTCCACCAGGAGCAGCAGCACTTCTCTGGCCAGCAGCACTCCCAGCCTGGAAAAC
  AAGCCTCCACAGGCTATTGTGAAACCACAGATTTTGACCCACGTTATAGAAGGCTTTGTGATTCAGGAGG
  GGCTGGAGCCGTTTCCTGTGAGTCGATCATCACTGCTGATAGAACAACCTGTGAAGAAGAGGCCTCTCTT
  GGATAATCAGGTGGTAAACTCTGTGTGTGTCCAGCCAGAGTTACAGAATAACACAAAGCATGCAGATAAC
  TCATCCGACACAGAGATAGAAGACATGATGGCTGAAGAGACATTGGAAGAAATGGATAGCGAGTTACTCA
  AGTGTGAATTCTGTGGAAAAATGGGATATCCTAATGAATTTTTACGGTCCAAACGATTCTGTACTATGTC
  ATGTGCCAAAAGGTACAACGTTAGCTGTTCTAAAAAATTTGCACTTAGTCGTTGGAATCGTAAGCCTGAT
  AATCAGAGTCTTGGGCATCGGGGCCGTCGTCCAAGTGGCCCTGAAGGGGCAGCAAGAGAACATATCCTTA
  GGCAGCTTCCAATTACTTATCCATCTGCAGAAGAAGACGTGGCCTCTCATGAAGATCCTGTGCCTTCTGC
  TATGACGACCCGTCTGCGAAGGCAGAGCGAGCGGGAAAGAGAGCGAGAGCTTCGGGATGTGAGGATTAGG
  AAGATGCCTGAGAATAGTGACTTGCTCCCAGTCGCACAGACTGAGCCCTCCATATGGACAGTCGACGACG
  TCTGGGCCTTCATCCATTCTTTGCCCGGCTGCCAGGACGTTGCAGATGAGTTCAGGGCACAAGAGATTGA
  TGGGCAGGCCCTGCTCTTGCTAAAAGAAGACCATCTCATGAGTGCAATGAACATGAAGCTGGGCCCAGCC
  CTGAAGATTTGTGCACGCATCAACTCTCTGAAGGACTCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001165954
• Insert Size: 2982 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165954.1, NP_001159426.1
• RefSeq Size: 11051 bp
• RefSeq ORF: 2982 bp
• Locus ID: 241915
• UniProt ID: Q8CHP6
• Cytogenetics: 3 A3
• Gene Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.