Med24 (NM_011869) Mouse Untagged Clone

CAT#: MC222996

Med24 (untagged) - Mouse mediator complex subunit 24 (Med24), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med24
• Synonyms: 100kD; 911GSE; AU040102; AW547152; D11Ertd307; D11Ertd307e; DRIP; DRIP100; Gse; Gse2; Pp; Pparb2; Pparbp2; R7552; R75526; Thr; Thrap4; Trap; Trap100
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222996 representing NM_011869
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTGGTGAACCTGAAGCAGGCCATTTTGCAAGCCTGGAAAGAGCGATGGAGTGACTACCAATGGG
  CAATCAACATGAAAAAATTCTTCCCCAAAGGAGCCACCTGGGACATTCTCAACCTAGCAGAAGCGCTCCT
  GGAGCAGGCTATGATTGGGCCTTCCCCCAATCCTCTGATCCTGTCGTACCTGAAGTACGCCATTAGTTCC
  CAGATGGTGTCCTGCTCCTCTGTGCTCACCGCCATCAGTAAGTTTGATGACTTTTCCCGGGACCTGTGTG
  TCCAGGCTCTGCTGGATATCATGGACATGTTCTGTGACCGACTCAGTTGCCACGGCAAAGCAGAGGAATG
  CATCGGGTTGTGCCGAGCGCTCCTTAGCGCCCTCCACTGGCTGCTTCGATGTACAGCAGCTTCTGCGGAG
  CGACTCCAGGAAGGGCTGGAGGCTGGCACTCCGGCCCCCGGGGAGAAGCAGCTTGCCTTGTGCTTGCAGT
  GCCTGGAGAAGACCCTCAGCAGCACCAAGAACCGAGCCCTGCTACACATCGCCAAACTAGAGGAGGCCTC
  CTCCTGGACTGCCATAGAGCATTCTCTTCTAAAACTTGGGGAGATCCTGGCCAATCTCAGCAACCCCCAG
  CTCCGGAGCCAAGCTGAGCGTTGCGGCACACTCATCAGGAGCATCCCCAGCATGCTGTCCGTGCACTCCG
  AGCAACTGCACAAGACCGGCTTCCCCACCATCCACGCACTCATCTTGCTTGAGGGCACCATGAACCTGAC
  CGGGGAGATGCAGCCCCTGGTGGAGCAGCTGATGATGGTGAAGCGCATGCAGCATATCCCCACACCTCTC
  TTTGTCCTGGAAATCTGGAAAGCTTGCTTTGTGGGACTCATTGAGTCCCCCGAGGGCACGCAGGAGCTGA
  AGTGGACAGCTTTCACCTACCTCAAGATTCCACAGGTCTTGGTGAAGTTGAAGAAGTACTTTCATGGAGA
  GAAGGATTTCACGGAGGATGTCAACTGTGCTTTTGAGTTCCTGCTGAAGCTCACCCCCTTGTTGGACAAA
  GCTGACCAGCGCTGCAATTGCGACTGCACGAACTTCCTCCTCCAGGAGTGCAACAAGCAGGGGCTTCTGT
  CTGAGGTCAACTTTGCCAGCCTTGTGGGCAAACGCACGGCAGATCGGGACCCGCAGCTAAAATCATCAGA
  AAATGCTAACATCCAGCCGAATCCTGGCCTGATCCTGCGGGCAGAGCCCACGGTCACAAACATTCTCAAG
  ACAATGGATGCAGATCACTCCAAGTCCCCGGAGGGACTGCTAGGGGTCCTGGGACATATGCTGTCTGGGA
  AGAGCCTGGATTTGCTGCTGGCTGCTGCCGCCGCCACTGGGAAGCTTAAATCCTTCGCCCGGAAATTCAT
  CAATCTGAATGAGTTCACGACCCACGGCAGTGGAGAGAGCACGAAAACAGCCTCGGTCCGAGCCTTGCTC
  TTTGACATTTCCTTCCTCATGCTATGCCATGTGGCCCAGACCTATGGCTCAGAGGTGATCCTCTCGGAGT
  CGAGCTCAGGAGAAGAAGTCCCCTTCTTTGAGACTTGGATGCAGACCTGCATGCCCGAGGAGGGCAAGAT
  TTTGAACCCTGACCATCCCTGCTTCCGCCCTGACTCCACCAAAGTCGAGTCCTTGGTGGCCCTGCTCAAC
  AACTCTTCAGAGATGAAGCTAGTACAGATGAAGTGGCACGAGGCCTGCCTCAGCATTTCAGCAGCCATTT
  TGGAAATCCTCAACGCTTGGGAGAATGGGGTTCTGGCATTTGAGTCCATCCAGAAAATCACCGATAATAT
  CAAGGGAAAGGTATGCAGTCTGGCGGTGTGTGCTGTGGCTTGGCTCGTGGCCCACGTCCGGATGCTGGGG
  CTGGATGAGCGTGAGAAGTCACTGCAGATGATCCGCCAGTTGGCAGGGCCACTGTACAGCGAGAACACCC
  TGCAGTTCTACAATGAGAGGGTGGTGATTATGAACTCAATCTTGGAACACATGTGTGCGGACGTGCTCCA
  GCAGACAGCCACGCAGATCAAGTTTCCATCCACAGGCGTGGACACGATGCCCTACTGGAACCTGCTTCCT
  CCCAAGCGCCCCATCAAGGAGGTCCTGACAGACATCTTTGCCAAGGTACTGGAGAAGGGCTGGGTGGACA
  GCCGCTCCATCCACATCCTTGACACCCTGCTGCATATGGGCGGCGTCTACTGGTTCTGCAACAACCTGAT
  TAAGGAGCTGCTGAAGGAGACTCGCAAGGAGCACACGTTAAGGGCGGTCCAGCTTCTCTACTCCATCTTC
  TGTCTGGACATGCAGCAAGTGACCCTGGTCCTGCTGGGTCACATCCTGCCCGGGCTGCTTACTGACTCTT
  CCAAGTGGCACAGCCTTATGGACCCTCCTGGCACTGCGCTGGCCAAACTGGCTGTGTGGTGTGCCCTGAG
  CTCTTACTCTTCCCACAAGGGACAGGCGTCCTCCCGCCAGAAGAAGAGACACAGAGAGGACATTGAGGAC
  TATGTCAGCCTCTTCCCCGTGGAGGACATGCAGCCCTCGAAGCTGATGCGACTCCTGAGCTCCAGTGACG
  ATGATGCCAACATCCTGTCAAGTCCCACTGACCGCTCCATGAACAGCTCCCTCTCAGCCTCCCAGCTCCA
  CACAGTCAACATGAGGGACCCTCTGAACCGAGTCCTGGCCAACCTGTTCCTGCTCATTTCCTCCATCTTG
  GGTTCACGCACCGCTGGCCCCCACACCCAGTTTGTGCAGTGGTTCATGGAGGAGTGTGTGGGCTGCCTGG
  AGCAGGACAGCAGGGGCAGCATTCTGCAGTTCATGCCCTTCACTACCGTATCAGAACTGGTGAAAGTGTC
  TGCTATGTCCAGTCCCAAGGTTGTTCTGGCCATCACAGACCTCAGCCTGCCCCTGGGCCGGCAGGTGGCC
  GCCAAAGCCATTGCTGCACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011869
• Insert Size: 2964 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011869.2, NP_035999.2
• RefSeq Size: 3427 bp
• RefSeq ORF: 2964 bp
• Locus ID: 23989
• UniProt ID: Q99K74
• Cytogenetics: 11 62.46 cM
• Gene Summary: This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene may form a submodule of the mediator complex that magnifies the effects of activators on the general transcription machinery. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]