Clcn1 (NM_013491) Mouse Untagged Clone

CAT#: MC222986

Clcn1 (untagged) - Mouse chloride channel 1 (Clcn1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clcn1
• Synonyms: adr; Clc-1; Clc1; mto; myotonia; nmf355; SMCC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222986 representing NM_013491
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGTCCCAGTCCCAGCGGCATGGAGGGGAACAGAGCTGGTGGGGCAGTGCCCCCCAGTACCAGT
  ACATGCCCTTTGAACATTGTACCAGCTACGGACTGCCCTCAGAGAATGGGGGCCTTCAGCACCGGCCCCG
  AAAGGACATGGGTCCCAGGCACAATGCCCACCCAACACAGATATATGGCCATCAAAAAGAACAATATTCA
  TATAAGGCACAGGACGGGGGAATGCCCAAGAAGATGGGCTCCAGTTCTACCATGGACAGCTTGGATGAGG
  ACCACTATTCTAAATGTCAAGACTGTGTCCATCGCCTGGGACGTGTGCTGAGAAGGAAGCTGGGGGAAGA
  TTGGATCTTTCTTGTGCTCCTGGGCCTCCTGATGGCTCTAGTCAGCTGGTGCATGGACTATGTTAGTGCC
  AAGAGCCTTCAGGCCTACAAGTGGACCTATGCCCAGATGAAGCCTAGCCTTCCTCTGCAGTACCTGGCCT
  GGGTCACCTTCCCACTTATCCTGATCCTCTTCAGCGCCCTCTTTTGCCAACTCATCTCTCCCCAGGCTGT
  GGGCTCTGGAATCCCTGAGATGAAAACAATTCTTCGTGGTGTTGTCTTGAAGGAATACCTCACACTCAAG
  GCCTTTGTAGCCAAGGTGGTAGCTTTGACAGCTGGACTGGGCAGTGGCATCCCTGTGGGGAAAGAGGGTC
  CCTTTGTTCACATCGCCAGCATCTGTGCTGCTGTCCTCAGCAAGTTTATGTCCATGTTCTCTGGTGTCTA
  TGAGCAGCCATACTATTACACTGACATCCTGACAGTGGGCTGTGCCGTGGGGGTCGGTTGCTGTTTTGGA
  ACACCACTTGGAGGAGTGCTATTTAGCATCGAGGTCACCTCTACCTACTTTGCTGTTCGGAATTACTGGC
  GAGGATTCTTTGCAGCCACATTCAGTGCCTTTGTGTTCCGTGTCCTGGCCGTTTGGAACAAGGATGCTGT
  CACCATCACTGCTCTGTTCAGAACGAATTTCCGAATGGATTTCCCCTTTGACCTGAAGGAACTCCCAGCT
  TTTGCTGTCATTGGGATTTGCTGCGGGTTCTTGGGAGCTGTTTTCGTTTATCTGCATCGCCAAGTCATGC
  TCGGTGTCCGAAAGCACAAGTGTCTCAGCCAATTTCTTGCTAAGCACCGCCTGCTATATCCTGGAATTGT
  TACCTTTGTCATCGCCTCGCTCACATTTCCACCAGGAATGGGTCAATTCATGGCTGGAGAGCTGATGCCC
  CGTGAAGCTATCAGTACCCTCTTTGACAACAACACATGGGTAAAGCACATAGGTGACCCCCAAAGCTTGG
  GCCAGTCAGCTGTGTGGCTTCACCCCCAGGTCAACGTGATCATCATCATCCTTCTCTTCTTCGTTATGAA
  GTTTTGGATGTCCATTGTAGCCACCACTATGCCCATACCCTGTGGAGGCTTCATGCCTGTTTTTGTGCTA
  GGAGCTGCATTTGGAAGGCTGGTAGGAGAGATCATGGCCATGCTATTCCCTGAGGGTATCTTATTTGATG
  ATATCATCTATAAGATCTTACCTGGGGGCTATGCAGTAATTGGAGCAGCAGCTTTGACAGGGGCTGTCTC
  CCACACAGTCTCCACAGCCGTCATTTGCTTCGAATTAACCGGTCAGATTGCTCACATCCTGCCCATGATG
  GTGGCTGTTATCTTGGCCAACATGGTGGCTCAGAGTCTGCAGCCCTCCCTCTATGACAGCATCATCCAGG
  TCAAGAAGCTTCCCTATCTGCCAGACCTTGGTTGGAACCAGCTCAGCAAATTTACAATTTTTGTTGAGGA
  CATCATGGTACGTGATGTGAAGTTCGTTTCAGCTTCTTGTACATATGGGGAACTGAGAAACCTACTCCAG
  GCCACCACAGTCAAGACTTTACCATTGGTTGACTCCAAAGATTCAATGATCCTGCTGGGCTCTGTGGAAC
  GCTCAGAACTGCAGTCCCTCCTGCAGCGCCACCTGTGTGCAGAGCGAAGGTTGAAGGCTGCCCAGGACAT
  GGCTCGAAAGTTATCAGAGCTGCCTTATAATGGCAAGGCTCAGCTGGCTGGGGACTGGCATCCTGGTGGT
  CGACCTGAGTCCTTTGCCTTCGTAGATGAAGATGAAGATGAGGACCTCTCCAGGAAGATGGAGCTTCCAC
  TGACTCCAGCTCCTCCCCCTCCTTCCCCTCCTCCTCCACCTTCCCAGTTTCCTATTGCTCCATCAAACCC
  TGAAGAACCTAATGGGCCACTGCCCAGCCATAAACAGCCACCTGAAGCCTCAGACTCTGCAGATCAAAGA
  TCATCCACCTTCCAACGCCTGCTGCACTGCTTGCTAGGAAAAGCTCACTCCAAAAAGAAGAAAATAACAC
  AGGATTCAACAGATTTAGTGGATAACATGTCACCTGAAGAGATTGAGGCCTGGGAGCGGGAGCAGCTGAG
  CCAGCCTGTGTGCTTTGATTGCTGCTGCATCGACCAGTCTCCCTTCCAGCTGGTGGAGCAGACAACCCTG
  CACAAGACTCATACACTCTTCTCACTTCTTGGCCTCCATCTTGCCTATGTGACCAGCATGGGGAAGCTCA
  GAGGTGTCTTGGCACTAGAAGAGCTACAGAAAGCTATTGAGGGCCACACCAAATCTGGGGTGCAGCTTCG
  CCCTCCACTTGCCAGCTTCCGGAATACAACTTCAATCCGGAAGACTCCTGGGGGGCCACCCCCTCCTGCA
  GAGGGCTGGAATGTACCTGAGGATGGAGATGGGGCTCCTGGAAGAGAAGTCATGGTTCCTACCATGCCAG
  AGACTCCTGTCCCACCACCATCTCCAGAGGCCCCTTCCTGCCTGGCCCCAGCCAGAGCGGAGGGTGAGCT
  GGAGGAACTGGAGATGGTGGGGAGCCTAGAGCCTGAGGAGGAGCTGGCTGACATCTTGCATGGCCCCAGT
  CTGCGGTCCACTGATGAGGAAGATGAGGACGAGCTGATCCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013491
• Insert Size: 2985 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013491.2, NP_038519.1
• RefSeq Size: 3545 bp
• RefSeq ORF: 2985 bp
• Locus ID: 12723
• UniProt ID: Q64347
• Cytogenetics: 6 20.57 cM
• Gene Summary: Voltage-gated chloride channel (By similarity). Plays an important role in membrane repolarization in skeletal muscle cells after muscle contraction (Probable) (PubMed:8119941).[UniProtKB/Swiss-Prot Function]