Phc3 (NM_153421) Mouse Untagged Clone

CAT#: MC222974

Phc3 (untagged) - Mouse polyhomeotic-like 3 (Drosophila) (Phc3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phc3
• Synonyms: E030046K01Rik; Edr3; Hph3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222974 representing NM_153421
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGTGAGCCGAGCTCTGGAACATCCGTGTCAACAACAGCCAGCAGTACCACCACCACCACCATCA
  CCACATCCTCCTCCCGAATGCAGCAGCCACAGATCTCTGTCTACAGCGGGTCAGACCGACATGCTGTTCA
  GGTAATTCAGCAGGCCTTGCATCGGCCCCCCAGCTCGGCAGCTCAGTACCTGCAGCAGATGTACGCGGCT
  CAGCAGCAGCACCTGATGCTGCACACGGCTGCTCTGCAGCAGCAGCACCTAAGCAGCTCGCAGCTCCAGA
  GCCTTGCGGCTGTCCAGGCAAGTTTGTCCAGTGGAAGACCATCTACATCCCCCACAGGCAGTGTTACACA
  ACAGTCAAGTATGTCCCAGACATCTATCCTGTCCGCTTCTCCTGCACCTGCTCAGCTGATGAACCGCTCC
  CAGACGTCCAGTTCTACCAGCGGCAGTATTACCCAGCAGACTATGCTACTCGGGAGTACCTCTCCCACCC
  TAACTGCCAGCCAAGCCCAGATGTATCTCCGAGCTCAGATGCTGATTTTCACACCTGCTACCACTGTGGC
  TGCTGTACAGTCTGACATCCCTGTTGTCTCGTCGTCACCGTCACCTTCCTGTCAGTCTGCAGCTGCTCAG
  GTTCAGAATTTAACCTTACGCAGCCAGAAGCTGGGTGTCTTATCTAGCTCACAGAATGGCTCACCAAAAA
  GCGCTGGTCAAACCCAGTCCTTGACAATTTGCCATAATAAAACAACAGTGACCAGTTCTAAAATCAGCCA
  ACGAGACCCTTCTCCAGAGAGCAAGAAGGGAGGAAGCCCAGGTCTGGAATCTCGGAGCACGGCCGTCACT
  CGGACATCAAGCATCCACCAGTTAATAGCACCAGCTTCATATTCTCCAATTCAGCCTCATTCTCTAATCA
  AACATCAGCAGATTCCTCTTCATTCACCACCTCCTAAAGTTTCCCATCATCAGCTGCTATTACAACAGCA
  ACAACAGCAAATTCAGCCAATCACCCTTCAGAGTCCAAGTCAAGATCCACCCCCATCCCAGCACTGTATC
  CCACTCCCAAACCATGGCCTTTCTCCGGCTCCCAGTAATGCCCAGCCGCAGCATTGCTCCCCAGTTCAGA
  GCCATCCTCCGCCTTTAACTGTGTCTCCCAACCAGGCACAGTCCGCACAGCAGTCTGTAGTGGTGTCTCC
  TCCCCCCCCTCATTCCCCAAGTCAGTCTCCTACTATAATTATCCATCCACAAGCACTTATCCAGCCACAC
  CCTCTTGTGTCTTCAGCTCTTCAAACAGGGCCAAATTTGCAGCAGGCTGCTGCTGATCAGGTACAGTCCA
  CCGCACAGCTGAATCTTCCATCCCATCTTCCACTTCCAGCATCCCCTGTTGTACACATTGGCCCTGTCCA
  GCAGTCTGCCTTGGTGTCCCCTGGTCAGCAGATGGTGTCCCCAACATCACACCAGCAGTATTCAGCCCTA
  CAGTCCTCTCCAATTCCCATCGCGACCCCTCCACAGATGTCGGCATCTCCTCCAGCTCAGCTCCCACCAC
  TGCCCTTGCAGTCTATGCAGTCTTTACAAGTGCAGCCTGAAATTCTATCCCAGGGCCAGGTTTTGGTGCA
  GAATGCTTTGGTGTCAGAAGAGGAGCTACCTGCTGCAGAAGCTTTGGTCCAGTTGCCATTTCAGACTCTT
  CCTCCTCCACAGACTGTTGCGGTAAATCTACAAGTACAGCCGCCAGCACCTGTTGATCCACCAGTGGTTT
  ATCAAGTAGAAGATGTGTGTGAGGAAGAAATGCCAGAGGAGTCCGATGAGTGTGCAAGGATGGACAGAAC
  CCCCCCACCCCCCACCTTGTCTCCAGCAGCTGTCACTGTGGGGAGGGGAGAGGACTTGACATCCGAACAT
  CCTTTGTTAGAGCAAGTGGAGTTGCCTGCTGTGGCCTCAGTCAGTGCTTCCGTGATCAAGTCTCCATCAG
  ACCCCACCCATGCCTCTGCTCCTGCACCTCCCCTCTTAATCCCTGCTGCCTCCACCAGGAGCAGCAGCAC
  TTCTCTGGCCAGCAGCACTCCCAGCCTGGAAAACAAGCCTCCACAGGCTATTGTGAAACCACAGATTTTG
  ACCCACGTTATAGAAGGCTTTGTGATTCAGGAGGGGCTGGAGCCGTTTCCTGTGAGTCGATCATCACTGC
  TGATAGAACAACCTGTGAAGAAGAGGCCTCTCTTGGATAATCAGGTGGTAAACTCTGTGTGTGTCCAGCC
  AGAGTTACAGAATAACACAAAGCATGCAGATAACTCATCCGACACAGAGATAGAAGACATGATGGCTGAA
  GAGACATTGGAAGAAATGGATAGCGAGTTACTCAAGTGTGAATTCTGTGGAAAAATGGGATATCCTAATG
  AATTTTTACGGTCCAAACGATTCTGTACTATGTCATGTGCCAAAAGGTACAACGTTAGCTGTTCTAAAAA
  ATTTGCACTTAGTCGTTGGAATCGTAAGCCTGATAATCAGAGTCTTGGGCATCGGGGCCGTCGTCCAAGT
  GGCCCTGAAGGGGCAGCAAGAGAACATATCCTTAGGCAGCTTCCAATTACTTATCCATCTGCAGAAGAAG
  ACGTGGCCTCTCATGAAGATCCTGTGCCTTCTGCTATGACGACCCGTCTGCGAAGGCAGAGCGAGCGGGA
  AAGAGAGCGAGAGCTTCGGGATGTGAGGATTAGGAAGATGCCTGAGAATAGTGACTTGCTCCCAGTCGCA
  CAGACTGAGCCCTCCATATGGACAGTCGACGACGTCTGGGCCTTCATCCATTCTTTGCCCGGCTGCCAGG
  ACGTTGCAGATGAGTTCAGGGCACAAGAGATTGATGGGCAGGCCCTGCTCTTGCTAAAAGAAGACCATCT
  CATGAGTGCAATGAACATGAAGCTGGGCCCAGCCCTGAAGATTTGTGCACGCATCAACTCTCTGAAGGAC
  TCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153421
• Insert Size: 2946 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153421.2, NP_700470.2
• RefSeq Size: 11066 bp
• RefSeq ORF: 2946 bp
• Locus ID: 241915
• UniProt ID: Q8CHP6
• Cytogenetics: 3 A3
• Gene Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream translational start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.