Aars2 (NM_198608) Mouse Untagged Clone

CAT#: MC222967

Aars2 (untagged) - Mouse alanyl-tRNA synthetase 2, mitochondrial (putative) (Aars2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aars2
• Synonyms: Aarsl; AlaRS; Gm89
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222967 representing NM_198608
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGGCGTTGGCTGCCGCAGCCGGTAAGCTGCGGCGAGCCATTGGGAGGTCGTGCCCATGGCAGC
  CTTTCTCAACCGAGCCCGGTCCACCCCACGGAGCGGCCGTGCGGGACGCCTTCCTGAGCTTCTTCCGAGA
  TCGCCACGGCCACCGGCTCGTGCCCTCCGCTACCGTGAGGCCGCGCGGCGACCCCAGCCTGCTTTTCGTC
  AATGCAGGCATGAACCAGTTCAAGCCCATCTTCCTGGGCACAGTGGATCCACGAAGTGAGATGGCAGGCT
  TCCGACGTGTAGTTAATAGCCAGAAGTGTGTCAGGGCTGGAGGACGCCATAACGACCTGGAGGATGTGGG
  GCGTGATCTCTCTCATCATACGTTTTTCGAGATGCTTGGCAACTGGGCTTTCGGGGGTGAATATTTTAAG
  GAGGAAGCTTGTAGCATGGCCTGGGAACTTCTGACTCAAGTCTACGGGATCCCTGAGGACAGGTTGTGGG
  TCTCCTACTTCAGTGGTGACTCCCAGACAGGACTGGACCCAGACCTGGAGACCAGAGACATCTGGCTAAG
  CTTGGGAGTGCCTGCCAGCCGTGTGCTCTCCTTCGGACCACAAGAGAACTTCTGGGAGATGGGAGACACT
  GGGCCTTGTGGGCCTTGTACTGAGATCCACTATGACCTGGCTGGGGGCGTGGGAAGCCCCCAGCTGGTAG
  AGCTTTGGAATCTGGTCTTCATGCAACACTACAGAGAGGCAGATGGAAGCCTGCAGCTGCTGCCGCAGCG
  ACATGTAGACACAGGAATGGGCCTGGAAAGGCTGGTGGCCGTCCTGCAAGGCAAACGTTCCACCTACGAC
  ACCGACCTCTTCTCTCCACTGCTCGACGCCATACACCAGAGCTGCGGGGCTCCCCCTTACTCTGGCCGGG
  TAGGGGCAGCAGATGAGGGACGGATAGACACAGCGTACCGAGTGGTAGCCGATCACATCCGCACACTCAG
  TGTCTGCATTGCTGACGGTGTCTCCCCGGGGATGTCAGGTGCCCCGCTAGTTCTCCGTCGGATTCTCCGC
  CGAGCTGTGCGCTATTCCACAGAGGTCTTGCAGGCACCTCCTGGCTTTCTAGGCAGCCTGGTGCCAGTGG
  TGGTAGAGACACTGGGATCTGCTTATCCAGAACTTGAGAAGAACTCAGTCAAGATAGCCAGCCTGGTGTC
  AGAGGATGAGGCAGCCTTCCTGGCCTCCCTGCAGCGGGGCCGGCGGATCATCGATCGCACCGTCAAGCGC
  CTGGGCCCTTCTGATTTGTTCCCTGCCGAAGTGGCCTGGTCATTGTCGCTGTCTGGGAATCTGGGGATTC
  CCCTGGACCTGGTGGAGCTGATGCTGGAGGAGAAGGGGGTGAAGCTGGACACGGCAGGACTGGAGCAGCT
  AGCCCAGAAGGAGGCTCAGCACCGGGCCCAGCAAGCAGAGGCAGATCAGGAGGACCGATTGTGTCTTGAT
  GTCCACGCACTGGAGGAGCTGCACCGTCAAGGCATACCCACAACTGATGACAGCCCCAAGTATAACTACA
  CTCTTCACCCCAACGGGGATTATGAGTTTGGCCTCTGTGAGGCCCGGGTGTTACAGCTGTATTCAGAGAC
  TGGGACAGCCGTGGCCTCCGTGGGAGCAGGCCAGCGCTGTGGCCTCCTATTAGATAGAACCAACTTCTAC
  GCTGAACAAGGGGGTCAGGCTTCAGACCGAGGCTACCTTGTCCGTACAGGGCAGCAGGACATGTTGTTCC
  CTGTGGCTGGGGCTCAGCTCTGTGGGGGTTTTATCCTGCATGAGGCCATGGCTCCCGAGCGCCTACAGGT
  GGGGGATCAAGTGCAGCTGTATGTAGATAAGGCCTGGCGAATGGGATGCATGGTGAAGCACACGGCCACC
  CACCTGCTGAGCTGGGCCCTTCGGCAGACCCTCGGACCGACCACCGAGCAGCGGGGCTCCCATCTCAACC
  CCGAGCGGCTGCGCTTTGACGTGGCCACCCAGACCCTACTAACCACAGAGCAGCTACGGACAGTAGAGAG
  CTACGTGCAGGAAGTCGTGGGGCAGGATAAGCCTGTGTTCATGGAGGAAGTGCCCCTGGCACACACTGCC
  CGCATCCCCGGCCTTCGCTCACTGGATGAGGTGTACCCAGACCCCGTCCGGGTGGTGTCCGTTGGGGTTC
  CTGTTGCCCACGCACTGGGGCCAGCCTCCCAGGCTGCAATGCACACCTCCGTGGAGCTGTGCTGTGGGAC
  GCACCTGCTAAGTACCGGGGCCGTGGGAGACTTGGTGATCATTGGGGAACGGCAGCTGGTCAAGGGCATC
  ACTCGCCTACTGGCCATCACTGGGGAGCAGGCCCAACAGGCCCGAGAGGTAGGCCAGAGCCTGTCTCAGG
  AGGTGGAAGCAGCCAGCGAGCGACTAAGTCAGGGCAGCCGAGACCTGCCGGAAGCTCACCGGCTATCTAA
  GGACATAGGACGTCTCACCGAGGTAGCAGAGTCTGCTGTGATACCTCAGTGGCAACGGCAGGAGCTGCAG
  ACCACACTGAAGATGCTACAGCGCCGTGCCAACACTGCCATCCGGAAACTGGAGAAGGGCCAGGCTACAG
  AGAAATCCCAGGAGCTGTTGAAACGACACTCGGAGGGGCCTCTGATTGTGGACACTGTCTCTGCCGAGTC
  CCTCTCAGTGCTGGTAAAGGTCGTGCGGCAGCTGTGCAAACAGGCCCCTAGCATATCCGTGCTGCTGCTC
  AGCCCCCAGCCCACTGGCAGTGTCCTGTGTGCCTGCCAGGTGGCCCAGGATGCCACGCCCACCTTCACTG
  CTGAAGCCTGGGCACTGGCTGTGTGCAGCCACATGGGAGGCAAGGCGTGGGGCTCCAGAGTGGTAGCTCA
  GGGGACTGGACACACGGCTGACCTGGAGGCCGCCCTCGGGACAGCCCGAGCTTACGCACTCAGCCAGCTC
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198608
• Insert Size: 2943 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198608.2, NP_941010.2
• RefSeq Size: 3368 bp
• RefSeq ORF: 2943 bp
• Locus ID: 224805
• UniProt ID: Q14CH7
• Cytogenetics: 17 B3
• Gene Summary: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.[UniProtKB/Swiss-Prot Function]