Myocd (NM_145136) Mouse Untagged Clone

CAT#: MC222964

Myocd (untagged) - Mouse myocardin (Myocd), transcript variant A, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myocd
• Synonyms: BSAC2A; Srfcp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222964 representing NM_145136
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACTCCTGGGGTCTGAACACTCTTTGCTGATTAGAAGGAAGTTCCGATCAGTCTTACAGTTACGGC
  TTCAACAGAGAAGGACCCAGGAGCAGCTGGCTAACCAAGGCTTAATACCGCCACTGAAAGGTCCAACTGA
  ATTCCATGACCCGAGAAAACAATTGGATAGTGCCAAGACTGAAGATTCCCTGAGGCGCAAGGGCAGAAAC
  AGGTCCGACCGTGCCAGCCTGGTTACTATGCACATTCTCCAAGCCTCCACGGCAGAAAGGTCCATTCCAA
  CTGCTCAGATGAAGCTCAAAAGAGCCCGCCTTGCAGATGACCTCAATGAGAAGATCGCTCTCCGCCCAGG
  GCCCTTGGAACTGGTGGAGAAGAACATTCTGCCGATGGATTCTTCCGTGAAAGAGGCTATAAAAGGTACT
  GAGGTGAGCCTCTCCAAGGCAGCAGATGCATTCGCCTTTGAGGATGACAGCAGTAGAGATGGGCTCTCTC
  CAGATCAGGCTAGGAGCGAGGACCCCCAGGGCTCTACAGGATCCACCCCAGACATCAAATCCACTGAGGC
  TCCTCTGGACACAATCCAGGATCTCACTCCTGGCTCAGAAAGTGACAAGAATGATGCAGCCTCCCAGCCA
  GGCAACCAGTCAGACCCTGGGAAGCAGGTTCTCGGCCCCCTCAGCACCCCGATTCCTGTGCACACTGCTG
  TAAAGTCCAAGTCTTTGGGTGACAGTAAGAACCGCCACAAAAAGCCCAAAGACCCCAAACCAAAGGTGAA
  GAAGCTCAAATACCATCAGTACATCCCCCCAGACCAGAAGGCAGAGAAGTCTCCCCCACCCATGGACTCT
  GCCTATGCCCGGCTGCTCCAGCAACAGCAGCTATTCCTGCAGCTACAGATCCTCAGCCAGCAGCAGCAAC
  AGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCGGTTCAGCTACCCTGGGAT
  GCACCAAACACACCTCAAAGAACCAAATGAACAGATGGCCAGAAATCCGAATCCTTCTTCAACACCACTG
  AGCAATACCCCTCTATCCCCTGTCAAAAATAGCATTTCTGGACAAACTGGTGTTTCTTCTCTCAAACCAG
  GCCCCCTCCCACCCAACCTGGATGATCTCAAGGTGTCAGAGTTAAGACAACAGCTTCGAATCCGGGGCTT
  GCCAGTGTCAGGCACCAAGACAGCGCTGGTGGACCGGCTTCGTCCCTTCCAGGATTGTGCTGGCAACCCT
  GTGCCCAACTTTGGGGACATCACAACTGTCACCTTTCCTGTCACGCCCAACACCTTGCCCAGTTATCAGT
  CCTCCCCGACAGGCTTCTACCACTTTGGCAGCACAAGCTCCAGCCCACCCATCTCCCCCGCCTCATCTGA
  CTTGTCCGCTGCAGGGTCCCTGCCAGACACCTTCACCGATGCGTCACCTGGCTTCGGCCTGCACGCATCT
  CCGGTGCCCGCCTGCACGGACGAGAGTCTGCTGAGCAGCCTGAATGGGGGCTCGGGCCCCTCCGAGCCTG
  ATGGGCTAGACTCTGAGAAGGACAAGATGCTGGTGGAGAAGCAGAAAGTGATCAACCAGCTCACCTGGAA
  GCTGCGGCAAGAGCAGCGGCAGGTGGAAGAGCTGAGAATGCAACTGCAGAAGCAGAAGAGCAGCTGCAGC
  GACCAGAAGCCACTGCCCTTCTTGGCCACCACCATCAAACAGGAAGATGTCTCCAGCTGCCCCTTCGCAC
  CCCAGCAGGCGTCTGGGAAGGGACAGGGCCACAGCTCTGACAGTCCCCCTCCGGCTTGTGAGACGGCTCA
  GCTGCTGCCTCACTGTGTGGAGTCCTCAGGTCAAACCCATGTACTCTCGTCCACGTTTCTCAGCCCCCAG
  TGCTCCCCTCAGCACTCGCCCCTGGGGGGCCTGAAGAGCCCGCAGCACATCAGCCTGCCTCCATCACCCA
  ACAACCATTACTTCCTGGCTTCCTCTTCGGGAGCTCAGAGAGAGAACCATGGGGTCTCTTCACCCAGCAG
  CAGCCAAGGGTGCGCACAGAACTCAGGGGCACACGAAGGCCATTCTTCTAGCTTCTCTTCCCCAGCTTCC
  AGCCTCCATCAGCCTTTCTCTGGCACCCAAGCAGACAGCAGTCACAGTGCTGGGCTCAACCCTTGTCCCA
  AAAGCCCAAGTATTCATCCAAAGATGACTGGTTTACAATCTTCTGACAAGGTGGGGCCAACGTTTTCAAT
  TCCATCCCCAACTTTTTCTAAGTCAAGTTCAGCAGTTTCAGATATCACCCAGCCCCCATCCTATGAAGAT
  GCAGTGAAGCAGCAAATGACTCGGAGTCAGCAGATGGACGAACTCCTGGATGTCCTCATTGAAAGTGGAG
  AAATGCCAGCCGATGCCAGGGAAGATCATTCATGTCTTCAGAAAATTCCAAAGATCCCTGGGTCCTCCTG
  CAGCCCAACTGCCATCCCCCCGAAGCCCTCGGCTTCCTTTGAGCAGGCATCTTCGGGAGGCCAGATGGCC
  TTCGATCACTACGCCAACGACAGTGACGAACACCTGGAAGTCTTATTGAATTCTCACAGCCCCATCGGAA
  AGGTGAGCGATGTTACCCTCCTCAAAATCGGAAGCGAGGAGCCTCCTTTTGACAGCATCATGGATGGCTT
  CCCAGGGAAGGCTGCGGAAGATCTCTTCAGTGCTCACGAGCTCTTGCCTGGGCCCCTCTCCCCGATGCAT
  GCACAGTTGTCACCTCCTTCTGTGGACAGCAGTGGTCTGCAGCTGAGCTTCACGGAATCTCCTTGGGAAA
  CAATGGAATGGCTGGACCTCACTCCACCTAGTTCCACGCCAGGCTTCAGCAACCTTACCTCCAGTGGGCC
  CAGCATTTTCAACATCGATTTTCTGGATGTTACAGATCTTAATCTGAATTCCCCTATGGATCTCCACTTA
  CAGCAGTGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145136
• Insert Size: 2952 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145136.4, NP_660118.3
• RefSeq Size: 5127 bp
• RefSeq ORF: 2952 bp
• Locus ID: 214384
• UniProt ID: Q8VIM5
• Cytogenetics: 11 B3
• Gene Summary: Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis). Isoform 1 mediates the cardiac transcription factor MEF2C-dependent transcription. Isoform 1 and isoform 3 are more active than isoform 2 and isoform 4 in stimulating cardiac muscle promoters.[UniProtKB/Swiss-Prot Function]