Pkn2 (NM_178654) Mouse Untagged Clone

CAT#: MC222959

Pkn2 (untagged) - Mouse protein kinase N2 (Pkn2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pkn2
• Synonyms: 6030436C20Rik; AI507382; PRK2; Prkcl2; Stk7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222959 representing NM_178654
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCAACCCCGACCGGGGGGAGATCCTCCTCACCGAGCTGCAGGGAGATTCCCGGACTCTTCCAT
  TTTCTGAGAATGTGAGTGCTGTTCAGAAATTAGACTTTTCAGATACAATGGTGCAACAGAAATTGGACGA
  CATCAAGGATCGAATAAAGAGAGAAATAAGGAAAGAACTGAAAATCAAAGAAGGAGCTGAAAACCTGAGG
  AAAGTTACAACAGATAAGAAAAATTTGGCTTATGTAGACAACATTTTGAAAAAATCAAATAAAAAATTAG
  AAGAATTACATCACAAGCTACAAGAATTAAATGCACACATCGTTGTATCGGATCCAGAGGACTCCACAGA
  TTGTCCGAGGACTCCAGATACTCCAAATAGTGACTCTCGTAGTTCTACTAGCAACAATAGACTGATGGCC
  TTACAGAAACAACTGGATATAGAACTTAAAGTAAAGCAGGGTGCAGAGAACATGATACAGATGTACTCAA
  ATGGCTCTTCAAAGGATCGGAAACTCCATGGTACAGCGCAGCAACTGCTACAGGATAGCAAGACAAAAAT
  AGAAGTCATCCGGATGCAGATTCTTCAGGCCGTCCAGACCAATGAGCTGGCTTTTGATAATGCAAAACCT
  GTGATAAGCCCTCTTGAGCTTCGGATGGAAGAATTAAGACATCATTTTAAGATAGAATTTGCAGTAGCCG
  AAGGTGCAAAGAATGTCATGAAGTTACTTGGCTCAGGAAAAGTAACAGACAGAAAAGCGCTTTCAGAAGC
  ACAAGCGAGATTTAATGAATCAAGTCAGAAGTTGGACCTTTTAAAGTATTCACTAGAGCAAAGGTTAAAT
  GAGCTCCCCAGGAATCACCCCAAAAGCAGTGTCGTCATTGAGGAGCTTTCACTGGTTGCGTCACCAACCC
  TGAGTCCACGGCAGAGCATGCTGTCCACGCAGAACCAGTATAGCACACTGTCCAAGCCAGCGGCGCTAAC
  CGGTACTTTGGAAGTTCGTCTTATGGGGTGCCAAGATATCCTAGAGAATGTACCTGGACGGTCAAAAGCA
  ACGTCGGTAGCACTACCTGGGTGGAGTCCAAGTGACAACAGATCGTCTTTTATGAGCAGAACCAGCAAGA
  GTAAAAGTGGGAGTAGTCGCAACCTCCTGAAAACGGATGACTTGTCCAATGATGTTTGTGCTGTTTTGAA
  GCTAGATAATACTGTGGTTGGTCAAACTAGCTGGAAACCCATTTCAAATCAGTCGTGGGACCAGAAGTTT
  ACACTGGAACTAGACAGGTCACGTGAACTGGAAATTTCAGTTTATTGGCGTGATTGGCGATCTCTGTGTG
  CTGTAAAATTTCTGAGGTTAGAAGATTTTTTAGACAACCAGCGGCATGGCATGTGTCTCTATTTGGAACC
  ACAGGGTACTTTGTTTGCAGAGGTTACTTTTTTTAATCCAGTTATTGAAAGACGACCAAAACTCCAAAGA
  CAAAAGAAAATTTTTTCAAAACAACAAGGCAAAACATTTCTCAGAGCTCCTCAGATGAATATTAATATTG
  CCACTTGGGGAAGACTAGTGAGACGAGCTATTCCCACAGTAAATCATTCTGGCACATTCAGCCCTCAGAC
  TCCTGTGCCTGCAACAGTGCCAGTCGTTGACGCACGCATTCCTGATCTAGCACCTCCGGCCAGTGACTCT
  ACAGTAACCAAATTGGACTTTGATCTTGAACCTGAACCTCCTCCAGCCCCACCTCGTGCTTCTTCCCTTG
  GAGAGACAGATGAATCTTCTGAATTGAGAGTTTTGGATATACCTGGACAGGGTTCAGAAACTGTTTTCAA
  TATTGAGAATGACAGAAATAACCTGCGTCCAAAATCCAAATCTGAATATGAGCTGAGCATTCCTGACTCA
  GGTCGAAGCTGCTGGGGTGTTGGAGAGCTTGATGACAAGAGAGCTCAGCAGAGGTTTCAGTTCAGTCTAC
  AGGACTTCAGGTGCTGTGCTGTCTTGGGGAGAGGACACTTTGGAAAGGTGCTGTTGGCGGAATATAAACA
  CACAAATGAAATGTTTGCAATAAAAGCCTTAAAGAAAGGAGACATTGTGGCACGAGATGAAGTAGACAGC
  CTGATGTGTGAAAAAAGAATTTTTGAGACTGTGAATAGTGTCAGGCACCCGTTTCTAGTGAACCTTTTTG
  CATGTTTCCAAACAAAAGAACATGTTTGCTTCGTGATGGAGTATGCTGCAGGTGGTGACCTGATGATGCA
  CATCCATACTGATGTCTTCTCTGAACCCAGAGCTGTATTTTATGCAGCATGTGTAGTTCTTGGGTTGCAA
  TACTTACATGAACACAAAATTGTTTATAGAGATTTGAAGTTGGATAACTTGTTGCTAGATACAGAAGGCT
  TTGTGAAAATTGCTGACTTTGGTCTTTGCAAAGAAGGAATGGGGTATGGAGATAGGACAAGCACATTTTG
  TGGTACTCCTGAGTTTCTTGCCCCAGAAGTCCTAACAGAAACATCGTACACAAGGGCTGTAGATTGGTGG
  GGCCTTGGTGTACTTATATATGAGATGCTCGTCGGCGAGTCTCCCTTTCCTGGTGATGATGAAGAAGAGG
  TTTTTGACAGTATTGTAAATGACGAAGTAAGGTATCCAAGGTTCTTATCTACAGAAGCCATTTCCATAAT
  GAGAAGGCTGCTAAGGAGAAATCCTGAGCGGCGCCTGGGAGCTGGTGAGAAGGATGCAGAGGATGTCAAG
  AAGCACCCGTTTTTCCGGCTAACCGACTGGAGTGCTCTAATGGACAAAAAAGTAAAGCCACCATTTGTAC
  CTACCATTAGAGGACGGGAAGATGTTAGTAACTTTGATGATGAATTTACCTCAGAAGCACCTATTCTGAC
  TCCACCTCGAGAACCAAGGATACTCTTGGAAGAGGAGCAGGAGATGTTCCACGATTTTGACTACGTTGCT
  GATTGGTGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178654
• Insert Size: 2952 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178654.4, NP_848769.2
• RefSeq Size: 6207 bp
• RefSeq ORF: 2952 bp
• Locus ID: 109333
• UniProt ID: Q8BWW9
• Cytogenetics: 3 66.69 cM
• Gene Summary: PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis (By similarity).[UniProtKB/Swiss-Prot Function]