Zbtb4 (NM_029348) Mouse Untagged Clone

CAT#: MC222952

Zbtb4 (untagged) - Mouse zinc finger and BTB domain containing 4 (Zbtb4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zbtb4
• Synonyms: 2310026P19Rik; 9230111I22Rik; mKIAA1538
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222952 representing NM_029348
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCCCTGCAGAGGTGACGGACCCATCCCATGCCCCAGCTGTCCTGCATCAGCTCAATGAGCAGC
  GGCTCCGTGGCCTCTTCTGTGACGTCACCCTCATAGCTGGAGACACCAAGTTCCCTGCTCACCGCAGTGT
  CCTGGCCGCCTCTAGTCCCTTCTTCAGAGAAGCCCTACTAGCTTCAGCCCCACTGCCCCTTCCACCAGTT
  ACTGGCGGCTCAGCCCCCAGCCCCGCAACCACCACAGCTGCCTCCTCTTCGTCCTCCTCTCCCCCTCCAG
  CCTCTCCTCACTCTTCATCTCCTCCCCGGGTGCTAGAGCTGCCCGGGGTCCCAGCAGCTGCCTTCTCCGA
  TGTCCTCAACTTCATCTATAGTGCCCGGCTTGCACTCCCTGGCGGTGGTGGGGATGGGGCAGCTGTGGCA
  GAGATTGGAGCTCTGGGCCGGAGGCTGGGTATCTCCCGTCTACAGGGCCTGGGGGAAGGAGGTGATACTT
  GGGTACCACCTGCCCCAACTTCCATGGTCACCTCAGATCCTACTGAGGATGGTTTGGGGGCAGGGCCTAG
  AACAGATGGAGAGTGGGTGGGTGACAAAGCTGAGGCCCTGACTCCTGACTCCCAGCCCCGGCGGCCCTTT
  CCTTGCCCTCGATGTGGAAAAAGCTTCATCCATCCCAAGCGACTACAGACCCACGAGGCTCAGTGTCGAC
  GAGGGTCCAACACTCGGGGGTCTGCAGGGCTGGGGCCTGGAGTCTCTGGCTCTGGGGGTCCTGCAGGAGT
  GGATGCCTCTGCCCTGCCACAACCAGTGGGCTTCAGAGATGGCCCTGAGCATGTGGTGAAGGTTGTGGGC
  GGCCACGTGCTCTATGTGTGTGCGGCCTGTGAGCGTTCCTACGTGACCCTGTCCAGCCTGAAGCGGCACA
  GCAATGTACACTCGTGGCGGAGGAAGTACCCCTGCCGCTACTGTGAGAAGGTGTTTGCCCTGGCTGAGTA
  CCGCACCAAGCACGAGGTGTGGCACACTGGGGAGCGCAGGTACCAGTGCATCTTCTGCTGGGACACCTTT
  GTCACCTATTATAACCTGAAGACCCACCAGCGAGCCTTCCATGGCATTAGCCCTGGCCTCCTAGCCAGTG
  AGAAGACACCCAATGGAGGCTACAAACCCAGGCTTAATACCCTCAAGCTGTACCGCCTGCTCCCTATGCG
  GGCGGCCAAGCGGCCCTACAAGACCTACAGTCAGGGAGCCCCTGAGGCCCCTCTTTCTCCAAGCCTCCAC
  ACACCGGCCCCCGCGGCAATGCCAGCCAGCCCCCAACCCCTACCCCCACCCGCCCCAGAGCCTGGTCCTC
  CCCCCTCTGTCATCACCTTTGCTCATCCGGCTCCCTCTGTCATTGTCCACGGGAGCAGTAGTAGTGGTGC
  AGCCGGGGGCGGACCAGCCGGCACAGGAGGGTCCCAAGCTGCCTCAGTCATCACTTATACGACTCCCCCA
  AGACCCCCCAAGAAACGAGAGTACCCACCTCCTCCCCCTGAGCCTACAGCAACACCCACCAGCCCAGCCT
  CCACCGCCGTCAGCCCAGCCACAGCTGCAGGGCCAGCCACAGCCACAGAGGAGGCCAAGGGCCGGAATCT
  GCGGGCTGGGAGGACTCTGACTTACACAGCCAAACCCGTGGGTGGGTTGAGTGGGAGTGGGGGTTCCCCC
  ACAGGGACTGGCCGAGGCTCTTCTCAGCTTCAGGCTCCACCTCCACTGTGTCAGATCACTGTGCGAATTG
  GGGAAGAGGCCATTGTCAAGCGCCGCATCTCAGAAACTGACCTGCGTCCTGGAGAGCTGAGTGGAGAGGA
  AGTAGAGGAGAGCGAGGAAGAGGAGGAGGAAGAGGAGGAGGAGGACCAGGAGGAACAAGAGGAATCGAAG
  GCTGGAGGGGAAGATCAGCTTTGGAGACCCTACTATTCATACAAGCCTAAGCGCAAGGCTGGAGCTACTG
  CCGGCGGGGCCAGCGGGGTCAGTGGACTGCCCCGAGGACGAAGACCACCACGCTGGAGGCAAAAGCTGGA
  ACGAAGGGGCTGGGAGGAGACCCCATCAGTGGAGGGCCCAGGAGGACGAGGACGTGGTGAGCGTAGGCAC
  CGTTGTGGGGACTGTGCCCAGGCCTTTGCCACTGTGAGGAAGCTGAGAAAGCACCAGGAAGCCCATAGTG
  GAGGCTCCCACACCTCCAGGACTGGGAGGAGGTCTTCCACCCGCTTCACCTGTCCCCACTGTGCCAAGGT
  GTGCAAGACGGCAGCTGCCCTGAACCGACATGGGCAAAGGCATGCTGTGGAACGGCCCGGGGGCACTCCC
  ACACCTGTCATTGCCTATTCCAAAGGAAGCATTGGCACCAGGCCCACTGACGTCAAGGAGGAGGCCCCCC
  AGGAGATGCAAGTGTCCTCTTCCAGTGGGGAGGCAGGCAGTGGTAGCGCTGCCGCTGCTGAAGCTTCTGA
  GTCTGCCTCACTCCAGGACCCGGTCATCTCTGGGGGTGAGGAGCCTCCAGTAGCAGGTGGGGGCAGCTAT
  GTATACCCACCTGTGCAGGAATTTCCCCTGGCCCTGATAGGAGGCAGCCGGGAGCCCAGTGCCGGCAAAG
  GAAAACCTGGGAATGAGGGTTCCTTGGGAGCTTCTGAGGGTGACCGGATGGAGGGGATGGGGACTGCCAA
  AGTCACCTTCTACCCTGAGCCCTACCCACTTGTCTATGGCCCCCAGCTCCTTGCTGCCTACCCTTACAAC
  TTCAGCAACTTGGCTGCTCTCCCAGTTGCTCTCAACATGGTCCTACCTGACGAAAAAGGTGGTGGGGCCC
  TTCCCTTCCTACCAGGGGTCTTTGGCTACGCAGTGAATCCTCAAGCGGCGCCACCCACTCCCCCTCCCCC
  TCTCCCTCTGCCAGTTTCCCCTAAGGGAATAGGGGGAATGACAGGGGTAGAGAGAACCCAGAAGGGGGAT
  GTGGGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029348
• Insert Size: 2949 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029348.2, NP_083624.2
• RefSeq Size: 7824 bp
• RefSeq ORF: 2949 bp
• Locus ID: 75580
• UniProt ID: Q5F293
• Cytogenetics: 11 B3
• Gene Summary: Transcriptional repressor with bimodal DNA-binding specificity. Represses transcription in a methyl-CpG-dependent manner. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Can also bind specifically to a single methyl-CpG pair and can bind hemimethylated DNA but with a lower affinity compared to methylated DNA. Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (PubMed:27446912).[UniProtKB/Swiss-Prot Function]