Clstn1 (NM_023051) Mouse Untagged Clone

CAT#: MC222947

Clstn1 (untagged) - Mouse calsyntenin 1 (Clstn1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clstn1
• Synonyms: 1810034E21Rik; Cst-1; Cstn1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222947 representing NM_023051
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGCCGCCCTGCGCCCGCGCTGGCCCCGGCCGTCCGGCTGCTGCTGGCGGGGCTGCTGTGCGGCG
  GCGGGGTCTGGGCCGCGCGAGTTAACAAGCACAAGCCATGGCTCGAGCCCACCTACCATGGGATAGTCAC
  CGAGAACGATAACACGGTGCTGCTCGACCCTCCCCTCATCGCCCTGGATAAAGATTCACCGCTCCGCTTT
  GCAGAGAGTTTTGAGGTGACAGTCACCAAAGAAGGTGAGATTTGTGGCTTTAAAATTCACGGGCAGAATG
  TCCCCTTTGATGCCGTGGTAGTGGATAAGTCCACGGGTGAGGGAATAATCCGGTCAAAAGAGAAACTGGA
  CTGTGAACTACAGAAAGACTACACATTCACCATCCAGGCCTACGACTGCGGGAAGGGCCCCGATGGCACC
  GGTGTGAAAAAGTCTCACAAAGCGACCGTGCACATCCAGGTGAACGACGTGAACGAGTACGCCCCCGTGT
  TCAAGGAGAAGTCGTACAAGGCGGCCGTGGTGGAGGGCAAGCAGCACAGCAGCATCCTCAGGGTGGAGGC
  TGTGGACGCAGACTGCTCCCCGCAGTTCAGCCAGATCTGCAGCTACGAGATTCTCACCCCAGACGTGCCG
  TTCACTGTGGACAAAGACGGTTATATTAAGAACACAGAGAAGCTAAACTATGGCAAAGAGCATCAGTATA
  AGCTCACAGTCACAGCCTACGACTGTGGGAAGAAAAGAGCCACCGAGGACGTCCTAGTGAAGATAAGCGT
  CAAGCCCACCTGCAGCCCCGGGTGGCAAGGCTGGAGCAGCAGAATTGAGTATGAGCCCGGCACGGGTGCT
  TTGGCTGTCTTCCCGAGCATCCACTTGGAGACCTGTGACGAGCCTGTCGCCTCTGTGCAGGCCACGGTGG
  AGCTGGAGACCAGCCACATAGGGAAAGGCTGCGACCGAGATACCTACTCTGAGAAGTCCCTTCACCGGCT
  CTGTGGGGCTGCTGCCGGCACCTCAGAGCTGCTCCCGTCCCCCAGTAGCTCCTTCAACTGGACTGTGGGC
  CTCCCCACCGACAACGGCCATGACAGTGACCAGGTCTTTGAGTTCAACGGCACGCAGGCCGTGAGGATTC
  CAGACGGCGTTGTGACCCTTGACCCTAAAGAACCCTTTACAATCTCCGTGTGGATGCGGCATGGGCCGTT
  TGGCCGGAAGAAGGAGACAATTCTCTGCAGTTCGGATAAGACAGATATGAACCGACACCATTATTCGCTC
  TACGTCCACGGGTGCAGGCTAGTTTTCCTCCTCCGTCAGGACCCGTCTGAGGAGAAGAAATACAGGCCTG
  CGGAGTTCCACTGGAAGCTGAACCAAGTCTGTGATGAGGACTGGCACCACTTTGTCCTCAATGTGGAAGT
  TCCAAGTGTGACTCTCTACGTGGACGGCATCCCTCATGAGCCCTTCTCTGTGACAGAGGACTACCCGCTT
  CATCCAACCAAAATAGAGACTCAGCTTGTGGTCGGAGCTTGCTGGCAAGAGTATTCAGGAGTGGAGAGTG
  GCAATGAGACTGAGCCTGCGACGATGGCCTCTGCAGGTGGCGACCTGCACATGACACAGTTTTTCCGAGG
  TAACCTGGCTGGCCTCACAGTCCGTTCTGGGAAACTGGCGGATAAGAAGGTGATTGACTGTCTCTACACC
  TGCAAGGAGGGGCTGGACCTGCAGGTCCCTGAAGATGCCAACAGAGGCGTGCAGATCCAAGCAAGCTCCA
  GCCAGGCGGTCTTGACCTTGGAGGGAGACAACGTTGGAGAGCTGGATAAGGCTATGCAGCACATTTCCTA
  CCTTAACTCAAGGCAGTTCCCCACACCAGGCATCCGCAGGCTCAAAATCACCAGCACCGTCAAGTGTTTC
  AACGAGGCGGCTTGCATCGAGGTGCCCCCGGTGGAAGGCTACGTGATGGTTTTGCAGCCGGAGGAGCCTA
  AGATCAGCCTGAGTGGCGTCCACCACTTTGCCCGCGCAGCTTCTGAGTTTGAGAGCGCGGAGGGTATTTC
  CCTCTTCCCTGAGCTGAGAATCATCAGCACCATCACCAGAGAGGTGGAGCCCGAGGCAGATGGGTCCGAG
  GACCCCACAGTGCAAGAATCCCTGGTGTCAGAGGAAATTGTGCATGACCTGGACACCTGTGAGGTCACAG
  TGGAAGGGGATGAGCTGAACGCAGAGCAGGAGAGCCTGGAGGTAGACGTGACCCGCCTCCAGCAGAAGGG
  CATCGAAGCAAGCCACTCTGACCTGGGTGTGGTCTTCACAGGCGTGGAGACCATGGCAAGCTACGAGGAG
  GTTTTGCACCTCCTTCGCTATCGGAATTGGCACACCAGGTCCCTGCTGGATCGCAAGTTCAAGCTCATTT
  GCTCAGAACTAAATGGTCGCTACCTCAGCAATGAGTTCAAGGTGGAGGTGAATGTGATCCACACAGCCAA
  CCCCGTGGAACATGCCAACCACATGGCTGCCCAGCCACAGTTCGTCCACCCTGAACATCGATCCTTTGTG
  GACCTGTCTGGTCACAACCTGGCCAATCCTCACCCGTTTGCAGTTGTCCCCAGCACGGCGACAGTGGTGA
  TTGTGGTGTGTGTCAGCTTCCTGGTTTTCATGATCATCCTGGGGGTGTTTCGGATCCGGGCTGCACATCA
  GCGAACGATGCGGGACCAGGACACGGGGAAGGAGAATGAGATGGACTGGGATGACTCTGCCTTGACCATC
  ACCGTCAACCCCATGGAGACATATGAGGACCAGCACAGCAGTGAGGAGGAGGAAGAGGAGGAGGAGGAGG
  AGGAGAGCGAAGATGGGGAGGAAGAGGAAGACATCACCAGTGCCGAGTCTGAGAGCAGTGAGGAGGAGGA
  AGGAGGCCCCGGAGACGGCCAGAATGCCACCCGACAGCTGGAATGGGATGACTCCACACTCAGCTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023051
• Insert Size: 2940 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023051.5, NP_075538.1
• RefSeq Size: 4505 bp
• RefSeq ORF: 2940 bp
• Locus ID: 65945
• UniProt ID: Q9EPL2
• Cytogenetics: 4 E2
• Gene Summary: Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. As intracellular fragment AlcICD, suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note:.