CD117 (NM_001122733) Mouse Untagged Clone

CAT#: MC222941

Kit (untagged) - Mouse kit oncogene (Kit), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: CD117
• Synonyms: belly; Bs; c-KI; c-KIT; CD117; Fdc; Gsfs; Gsfsco1; Gsfsco5; Gsfsow3; SC; SCO1; SCO5; SO; SOW3; Ssm; Tr-k; Tr-kit; W
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222941 representing NM_001122733
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGGCGCTCGCGGCGCCTGGGATCTGCTCTGCGTCCTGTTGGTCCTGCTCCGTGGCCAGACAGCCA
  CGTCTCAGCCATCTGCAAGTCCAGGGGAGCCGTCTCCGCCATCCATCCATCCAGCACAATCAGAGTTAAT
  AGTTGAAGCTGGCGACACCCTCAGCCTGACGTGCATTGATCCCGACTTTGTCAGATGGACTTTCAAGACC
  TATTTCAATGAAATGGTTGAGAATAAAAAAAATGAATGGATCCAGGAAAAAGCCGAGGCCACTCGCACGG
  GCACATACACGTGCAGCAACAGCAATGGCCTCACGAGTTCTATTTACGTGTTTGTTAGAGATCCTGCCAA
  ACTTTTCCTGGTTGGCCTTCCCTTGTTTGGCAAAGAAGACAGCGACGCGCTGGTCCGCTGCCCTCTGACA
  GACCCACAGGTGTCCAATTATTCCCTCATCGAGTGTGATGGGAAATCTCTCCCCACGGACCTGACGTTTG
  TCCCAAACCCCAAGGCTGGCATCACCATCAAAAACGTGAAGCGCGCCTACCACCGGCTCTGTGTCCGCTG
  TGCTGCTCAGCGTGACGGTACATGGCTGCATTCTGACAAATTCACCCTCAAAGTGCGGGCAGCCATCAAG
  GCTATCCCTGTTGTGTCTGTGCCTGAAACAAGTCACCTCCTTAAGAAAGGGGACACATTTACGGTGGTGT
  GCACCATAAAAGATGTGTCTACATCCGTGAACTCCATGTGGCTAAAGATGAACCCTCAGCCTCAGCACAT
  AGCCCAGGTAAAGCACAATAGCTGGCACCGGGGTGACTTCAATTATGAACGCCAGGAGACGCTGACTATC
  AGCTCGGCAAGAGTTGACGATTCTGGAGTGTTCATGTGTTATGCCAATAATACTTTTGGATCAGCAAATG
  TCACAACAACCTTGAAAGTAGTAGAAAAAGGATTCATCAACATCTCCCCTGTGAAGAACACTACAGTATT
  TGTAACCGATGGAGAAAACGTAGATTTGGTTGTTGAATACGAGGCCTACCCCAAACCCGAGCACCAGCAG
  TGGATATATATGAACAGGACCTCGGCTAACAAAGGGAAGGATTATGTCAAATCTGATAACAAAAGCAACA
  TCAGATATGTGAACCAACTTCGCCTGACCAGATTAAAAGGCACAGAAGGAGGCACTTATACCTTTCTGGT
  GTCCAACTCTGATGCCAGTGCTTCCGTGACATTCAACGTTTACGTGAACACAAAACCAGAAATCCTGACG
  TACGACAGGCTCATAAATGGCATGCTCCAGTGTGTGGCAGAGGGATTCCCGGAGCCCACAATAGATTGGT
  ATTTTTGTACAGGAGCAGAGCAAAGGTGTACCACTCCTGTCTCACCAGTGGACGTACAGGTCCAGAATGT
  ATCTGTGTCACCATTTGGAAAACTGGTGGTTCAGAGTTCCATAGACTCCAGCGTCTTCCGGCACAACGGC
  ACGGTGGAGTGTAAGGCCTCCAACGATGTGGGCAAGAGTTCCGCCTTCTTTAACTTTGCATTTAAAGGTA
  ACAACAAAGAGCAAATCCAGGCCCACACTCTGTTCACGCCGCTGCTCATTGGCTTTGTGGTTGCAGCTGG
  CGCGATGGGGATCATTGTGATGGTGCTCACCTACAAATATTTGCAGAAACCCATGTATGAAGTACAATGG
  AAGGTTGTCGAGGAGATAAATGGAAACAATTATGTTTACATAGACCCGACGCAACTTCCTTATGATCACA
  AATGGGAGTTTCCCAGAAACAGGCTGAGTTTTGGAAAGACATTGGGAGCTGGTGCCTTCGGGAAGGTCGT
  TGAGGCCACTGCATATGGCTTGATTAAGTCGGATGCTGCCATGACAGTTGCCGTGAAGATGCTCAAACCA
  AGTGCCCATTTAACAGAAAGAGAGGCCCTAATGTCGGAACTGAAGGTCCTGAGCTACCTGGGCAATCACA
  TGAATATTGTGAACCTGCTTGGCGCATGCACGGTGGGAGGGCCCACCCTGGTCATTACAGAATATTGTTG
  CTATGGTGATCTTTTGAATTTTTTGAGAAGGAAGCGTGACTCGTTTATTTTCTCAAAGCAAGAAGAGCAG
  GCAGAAGCGGCACTTTATAAGAACCTTCTGCACTCAACGGAGCCTTCCTGTGACAGTTCAAATGAATATA
  TGGACATGAAGCCTGGCGTTTCCTACGTGGTGCCAACCAAGACAGACAAGAGGAGATCCGCAAGAATAGA
  CTCGTACATAGAAAGAGACGTGACTCCTGCCATCATGGAAGATGACGAGCTGGCTCTGGACCTGGATGAT
  TTGCTGAGCTTCTCCTACCAGGTGGCCAAGGGCATGGCGTTCCTCGCCTCCAAGAATTGTATTCACAGAG
  ATTTGGCAGCCAGGAATATCCTCCTCACTCACGGGCGGATCACAAAGATTTGCGATTTCGGGCTAGCCAG
  AGACATCAGGAATGATTCGAATTACGTGGTCAAAGGAAATGCACGACTGCCCGTGAAGTGGATGGCACCA
  GAGAGCATTTTCAGCTGCGTGTACACATTTGAAAGTGATGTCTGGTCCTATGGGATTTTCCTCTGGGAGC
  TCTTCTCCTTAGGAAGCAGCCCCTACCCAGGGATGCCGGTCGACTCCAAGTTCTACAAGATGATCAAGGA
  AGGCTTCCGGATGGTCAGCCCGGAGCACGCGCCTGCCGAAATGTATGACGTCATGAAGACTTGCTGGGAC
  GCTGACCCCTTGAAAAGGCCAACATTCAAGCAGGTTGTCCAACTTATTGAGAAGCAGATCTCGGACAGCA
  CCAAGCACATTTACTCCAACTTGGCAAACTGCAACCCCAACCCAGAGAACCCCGTGGTGGTGGACCATTC
  CGTGAGGGTCAACTCGGTGGGCAGCAGCGCCTCTTCTACGCAGCCCCTGCTCGTGCACGAAGATGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001122733
• Insert Size: 2940 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122733.1, NP_001116205.1
• RefSeq Size: 5205 bp
• RefSeq ORF: 2940 bp
• Locus ID: 16590
• UniProt ID: P05532
• Cytogenetics: 5 39.55 cM
• Gene Summary: The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).