Vps54 (NM_139061) Mouse Untagged Clone

CAT#: MC222927

Vps54 (untagged) - Mouse vacuolar protein sorting 54 (yeast) (Vps54), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps54
• Synonyms: Hcc8; mSLP8; Vps54l; wr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222927 representing NM_139061
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCAAGCCACAGTTCTTCTCCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTTAAAAAAGAGG
  TAGATCCAACAAAACACATTCGACCTGTGCAGTCACTGCCAGATGTGTGTCCCAAGGAACCCACAGGTGA
  TTCACATACTTTATGTGTTGCCCCATCTCTAGTTACAGATCAACATAGATGGACTGTGTATCATTCCAAA
  GTAAATCTCCCAGCAGCATTAAATGATCCTACACTAGCAAAAAGAGAATCTGACTTCTTCACAAAAACTT
  GGGGATTGGACTTTGTGGACACTGAAGTCATACCTTCACTCTACCTCCCACAGATCAGCAAGGAGAATTT
  TATAGCATATCAGCAGGAGATCTCTCAGAGAGAGAAGATTCATGAGAGATGCAAGAATATTTGTCCTCCT
  AAAGATACCTTTGACAGGACTCTTTTACATATTCATGATAAATCCAGGACAGATCTGGAGCAAGTACCTA
  AGATTTTTATGAAACCAGATTTTGCCTTGGATGATTCTTTAACTTTTAATTCAGTTTTACCATGGTCTCA
  TTTTAATACTGCTGGTGGGAAAGGAAGTCGCGACGCAGCTTCTTCCAAGTTGCTTCAGGAAAAGCTGAGC
  CATTATCTGGATATTGTGGAAGTAAACATTGCTCACCAGATCTCTCTACGTTCAGAAGCATTTTTTCATG
  CAATGACCTCTCAACACGAATTGCAGGACTACCTCAAGAAAACTACCCAGGCTGTAAAAATGCTTCGAGA
  TAAAATTGCACAGATTGATAAAGTTATGTGTGAAGGATCACTACAGATTTTAAGACTGGCACTTACCAGA
  AATAATTGTGTTAAAGTATACAATAAACTGAAGTTAATGGCCACTGTACACCAAACTCAGCCTACAGTGC
  AGGTGTTGTTATCTACTTCTGAATTTGTTGGAGCGTTGGACTTAATAGCAACAACTCAAGAGGTTCTGCA
  GCAGGAGCTTCAGGGCATTCACAGCTTCCGGCATTTGGGATCACAGCTTTGTGAATTAGAAAAGCTGATT
  GATAAAATGATGATTGCAGAATTTTCTACTTATTCTCATAGTGACTTAAATAGACCACTGGAAGGTGAAT
  GCCAAGTTTTAGAAGAGGAAAGATTAGTATCTCTTGTATTTGGGCTTTTGAAACAAAGAAAACTTAATTT
  TTTAGAAATCTATGGTGAAGAAATGATTATTACAGCAAAGAACATCATTAAAGAGCGTGTGATTAATAAA
  GTTTCACAAATAGAAGAAATAGACACAGACGTTGTTGTAAAGCTTGCAGATCAAATGAGAATGTTGAATT
  TTCCCCAGTGGATTGATCTGTTAAAGGACATTTTCTCTAAGTTTACAGTTTTCCTACAGAGAGTTAAGGC
  AACATTGAATATTATCCACAGTGTTGTTCTGTCAGTTCTTGAGAAAAGCCAAAGGACTAGAGAATTGGAA
  GAGATCCCACAGCAGAGGAGCGCTGGGAAGGACAGCTCCTTGGACACAGACGTGGCTTACCTGACACACG
  AGGGCTGGTTCATAAGTGATGCTTTCAGTGAGGGTGAGCCAGCATCTGCGGCGGTTGATACTACCTCTCA
  AAGAAACACGTCTCCACATAGCGAGCCCTGCAGCAGTGATTCGGTATCAGAACCAGAATGTACTACTGAT
  TCTTCATCCAGCAAGGAGCAGACATCAGCGTGTGCTCCTCCAGGAGGTATAGAGATTATAGTCAGTGAAG
  ACATGAGGTTAACTGACTTGGAGCTAGGAAAACTAGCAAGTAACATCCAGGAGTTACTGTGTAATGCTTC
  AGATGTATGCCATGATCGAGCTGTCAAATTTCTCATGTCAAGAGCAAAGGACGGTTTTCTTGAAAAATTG
  AATTCCACGGAATTCATAGCACTCTCGAGATTAATGGAGACATTCATAGTGGACACTGAACAGATCTGTG
  GGAGGAAGAGCACGTCACTGCTTGGGGCCCTTCAGAGCCAAGCAAATAAGTTTGTGAACAGGTTTCATGA
  AGAGAGGCGGACCAAGCTCAGCCTCCTCTTAGACAACGAGCGTTGGAAGCAGGCTGATGTTCCTGCAGAA
  TTTCAGGATCTTGTTGATTCTATAGCGGATGGAAAGATTGCTTTACCTGAAAAAAAGCCAGTGGTTACAG
  AAGAAAGGAAGCCAGCTGATGTTCTTGTTGTTGAGGGACACCAGTATGCAGTTGTCGGAACCGTCTTGCT
  GTTAATAAGAATCATTCTTGAATATTGCCAGTGTGTGGATAACATCCCCTCCGTCACTACTGACATGCTC
  ACTCGCCTGACAGATCTGCTGAAGTACTTCAATTCAAGAAGTTGCCAGTTAGTTCTTGGAGCTGGTGCTT
  TGCAAGTTGTTGGACTAAAAACAATTACTACAAAAAACTTGGCCCTCTCTTCACGGTGTCTGCAGTTGAT
  TGTGCACTACATTCCCGTGATCCGGGCTCACTTTGAAGCCCGACTGCCCCCCAAGCAATGGAGCATGCTT
  AGACATTTTGATCACATCACAAAGGATTACCATGATCATATAGCTGAAATTTCAGCTAAGCTTGTAGCGA
  TAATGGATAGCCTATTTGATAAGCTGTTATCAAAGTATGAGGTGAAAGCTCCTGTTCCTTCCCCGTGTTT
  CAGGAATATTTGTAAGCAAATGACAAAAATGCATGAAGCTATATTTGATCTTCTTCCCGAAGAACAAACA
  CAGATGTTATTTTTAAGAATTAATGCAAGTTACAAACTCCACTTGAAAAAACAGTTATCACACTTAAATG
  TAATAAATGACGGAGGACCTCAAAATGGGTTGGTCACAGCAGATGTAGCTTTTTACACTGGAAATCTTCA
  AGCCTTAAAAGGCCTTAAAGATCTGGATCTAAATATGGCCGAGATCTGGGAACAGAAGAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_139061
• Insert Size: 2934 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139061.5, NP_620692.3
• RefSeq Size: 4569 bp
• RefSeq ORF: 2934 bp
• Locus ID: 245944
• UniProt ID: Q5SPW0
• Cytogenetics: 11 13.89 cM
• Gene Summary: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).