Piwil2 (NM_021308) Mouse Untagged Clone

CAT#: MC222906

Piwil2 (untagged) - Mouse piwi-like homolog 2 (Drosophila) (Piwil2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Piwil2
• Synonyms: mili; Piwil1l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222906 representing NM_021308
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCTGTCAGGCCGTTGTTCAGGGGGCCCACCCCAGTCCACCCATCTCAGTGTGTGCGGATGCCAG
  GCTGTTGGCCTCAAGCTCCTAGACCTTTGGAACCAGCTTGGGGTAGGGCAGGACCTGCAGGCAGAGGCCT
  TGTGTTTAGAAAACCAGAAGACTCCAGCCCACCACTCCAGCCAGTACAAAAGGATTCTGTAGGTTTGGTG
  TCCATGTTCCGTGGCATGGGTCTTGACACAGCATTCCGGCCTCCTTCAAAACGAGAAGTGCCTCCTTTAG
  GCAGAGGAGTTCTAGGTCGAGGCTTGTCTGCTAACATGGTCCGCAAGGACAGAGAAGAACCCCGTTCCTC
  TTTGCCTGATCCTTCGGTGCTGGCAGCTGGGGACAGCAAACTGGCAGAGGCTTCTGTTGGTTGGAGTAGA
  ATGCTGGGAAGAGGTAGTTCTGAGGTCTCTCTGTTACCACTGGGACGAGCAGCCAGTAGTATAGGCAGAG
  GAATGGACAAACCTCCCAGTGCCTTTGGCCTCACAGCTCGGGATCCCCCACGGCTGCCACAGCCTCCAGC
  TCTGTCTCCAACTTCACTGCACTCTGCCGATCCCCCTCCGGTCCTGACTATGGAACGAAAGGAAAAAGAG
  CTTTTGGTCAAGCAAGGATCAAAAGGAACTCCTCAGTCTTTGGGACTGAACCTCATCAAAATCCAGTGTC
  ATAACGAAGCAGTTTATCAGTACCATGTGACTTTCAGCCCCAGTGTGGAATGCAAAAGCATGAGGTTTGG
  CATGTTGAAGGACCACCAGTCTGTCACTGGAAACGTCACTGCTTTTGATGGCTCTATTCTTTATCTTCCT
  GTTAAGCTTCAACAAGTTGTTGAGTTAAAAAGTCAGAGGAAAACTGACGATGCCGAGATCAGTATCAAGA
  TTCAGCTGACAAAGATCCTGGAGCCGTGTTCTGACCTGTGCATCCCCTTCTACAATGTTGTCTTCCGGCG
  GGTAATGAAACTTCTGGATATGAAGCTTGTGGGGAGAAACTTCTATGACCCTACAAGTGCCATGGTACTG
  CAGCAACACAGATTGCAGATCTGGCCTGGCTATGCGGCTAGTATCCGGAGGACAGACGGGGGTCTCTTCC
  TGCTCGCTGATGTCTCTCATAAGGTCATTCGGAACGACTCTGTGCTGGATGTCATGCATGCTATCTACCA
  GCAGAACAAGGAGCACTTCCAGGACGAGTGCAGCAAGCTTCTGGTTGGCAGCATTGTCATCACGCGCTAC
  AACAATCGTACCTACCGAATCGATGATGTGGACTGGAACAAGACCCCTAAAGACAGCTTTGTCATGTCGG
  ACGGGAAGGAAATCACATTCCTGGAATACTACAGCAAAAACTATGGGATCACAGTCAAGGAAGATGACCA
  GCCGCTGCTGATCCACCGGCCCAGTGAGAGACAGAATAACCATGGCATGTTGCTGAAGGGCGAGATCCTG
  CTGCTGCCCGAGCTCTCCTTCATGACGGGGATCCCTGAGAAGATGAAGAAGGACTTCAGGGCCATGAAGG
  ACTTGACTCAGCAGATTAACCTGAGCCCCAAGCAGCACCACGGTGCTTTGGAATGCCTGCTGCAGAGAAT
  TTCACAAAACGAGACAGCCAGCAATGAGCTGACCCGCTGGGGGCTCAGTCTGCATAAAGATGTCCACAAG
  ATTGAAGGTCGGCTTCTGCCAATGGAGAGGATCAACTTAAGGAACACTTCATTTGTCACATCGGAGGACC
  TGAACTGGGTTAAGGAAGTGACCAGAGATGCTTCCATTCTAACTATTCCCATGCATTTCTGGGCACTCTT
  TTATCCAAAGAGAGCAATGGACCAAGCCAGAGAACTGGTTAACATGTTGGAAAAGATTGCCGGGCCCATT
  GGCATGCGCATAAGCCCCCCAGCCTGGGTTGAGCTGAAGGATGACCGAATAGAGACCTATATCAGGACCA
  TTCAGTCCTTACTGGGAGTTGAGGGGAAGATACAAATGGTCGTTTGCATCATCATGGGCACACGTGATGA
  TCTCTATGGAGCCATCAAGAAGCTGTGCTGCGTGCAGTCCCCAGTGCCCTCACAGGTCATCAATGTCCGA
  ACCATTGGTCAGCCCACCAGGCTTCGGAGCGTGGCTCAGAAAATTTTACTTCAGATGAACTGTAAACTGG
  GTGGTGAGCTCTGGGGAGTGGATATTCCGCTGAAACAACTAATGGTGATTGGAATGGATGTGTACCATGA
  CCCCAGCAGAGGCATGCGCTCTGTGGTCGGCTTCGTGGCCAGCATAAATCTCACACTCACCAAATGGTAC
  TCGAGGGTGGTGTTCCAGATGCCACATCAGGAGATTGTGGACAGCCTGAAGCTCTGCCTGGTGGGTTCCT
  TGAAAAAGTATTATGAGGTGAACCATTGTCTCCCAGAGAAAATTGTGGTGTACCGAGATGGAGTGTCTGA
  TGGCCAGCTAAAGACAGTTGCCAACTACGAGATCCCTCAGCTGCAGAAGTGTTTTGAAGCCTTTGATAAC
  TACCACCCCAAGATGGTGGTGTTTGTAGTTCAGAAGAAAATCAGCACCAATCTGTACCTTGCTGCTCCTG
  ATCACTTCGTAACCCCCTCCCCCGGGACTGTGGTTGATCATACCATAACCAGCTGTGAGTGGGTGGATTT
  CTACCTTCTTGCCCATCATGTGCGACAGGGCTGTGGCATACCTACACACTACATCTGTGTTCTGAACACT
  GCAAATCTGAGCCCTGATCACATGCAGAGGTTGACTTTCAAACTATGCCACATGTACTGGAATTGGCCTG
  GTACCATCCGAGTTCCAGCTCCTTGCAAGTATGCCCACAAGCTAGCTTTCCTGTCCGGACAGATTTTGCA
  TCATGAGCCAGCCATCCAGCTGTGTGGGAACCTGTTCTTCCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021308
• Insert Size: 2916 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021308.1, NP_067283.1
• RefSeq Size: 4913 bp
• RefSeq ORF: 2916 bp
• Locus ID: 57746
• UniProt ID: Q8CDG1
• Cytogenetics: 14 D2
• Gene Summary: Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:11578866, PubMed:14736746, PubMed:17446352, PubMed:18381894, PubMed:18922463, PubMed:26669262). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (PubMed:11578866, PubMed:14736746, PubMed:17446352, PubMed:18381894, PubMed:18922463, PubMed:26669262). Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females (PubMed:11578866, PubMed:14736746, PubMed:17446352, PubMed:18381894, PubMed:18922463, PubMed:26669262). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (PubMed:11578866, PubMed:14736746, PubMed:17446352, PubMed:18381894, PubMed:18922463, PubMed:26669262). During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4 (PubMed:22020280, PubMed:23706823, PubMed:26669262). PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded (PubMed:28633017). Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense (PubMed:18381894, PubMed:18922463, PubMed:26669262). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (PubMed:19114715). Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8 (PubMed:16261612). Represses circadian rhythms by promoting the stability and activity of core clock components ARNTL/BMAL1 and CLOCK by inhibiting GSK3B-mediated phosphorylation and ubiquitination-dependent degradation of these proteins (PubMed:28903391).[UniProtKB/Swiss-Prot Function]