Reck (NM_016678) Mouse Untagged Clone

CAT#: MC222903

Reck (untagged) - Mouse reversion-inducing-cysteine-rich protein with kazal motifs (Reck), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Reck
• Synonyms: mRECK; St15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222903 representing NM_016678
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCGTCCGGGCCTCCCCGCGCAGCGCGCTGCTACTTCTGCTGGCCGCGGCGGGGGTCGCGGAGG
  TGACGGGGGGCCTGGCCCCGGGCAGCGCGGGTGCTGTATGCTGTAATCATTCAAAAGATAACCAAATGTG
  CCGTGATGTATGTGAGCAGATTTTCTCCTCAAAAAGTGAATCCCGACTGAAACATCTGTTGCAGAGAGCC
  CCAGATTATTGCCCTGAAACAATGGTTGAAATTTGGAGTTGCATGAATTCGTCTTTGCCAGGGGTATTTA
  AGAAGTCCGATGGTTGGGTTGGCTTAGGCTGCTGTGAACTGGCTATTGGCTTGGAGTGCCGACAGGCATG
  CAAGCAGGCATCTTCGAAAAATGATATTTCCAAAGTTTGCAGGAAAGAATATGAGAATGCTCTTTTCAGT
  TGCATTAGCAGAAATGAAATGGGCTCGGTGTGCTGCAGTTACGCAGGCCATCACACCAACTGCCGAGAAT
  TCTGTCAAGCCATTTTTCGAACAGACTCTTCTCCTGGGCCATCTCAGATCAAAGCAGTGGAAAATTATTG
  TGCCTCTATCAGTCCACAGTTGATACACTGTGTGAATAATTACACCCAGTCTTATCCAATGAGAAACCCA
  ACTGATAGTTTATATTGCTGTGACAGAGCTGAAGACCATGCTTGCCAAAACGCCTGCAAGAGAATTCTAA
  TGTCTAAGAAAACAGAAATGGAGATTGTTGATGGTCTCATTGAGGGTTGTAAGACCCAGCCCTTGCCTCA
  AGATCCTCTTTGGCAGTGTTTTCTCGAAAGCTCACAGTCGGTTCACCCTGGAGTCACTGTGCACCCTCCT
  CCCTCAACTGGCCTCGACGGGGCCAAATTGCACTGTTGCTCTAAAGCAAACACTTCAACCTGTAGGGAAC
  TGTGCACCAAACTTTATAGCATGAGCTGGGGAAATACACAGAGTTGGCAAGAGTTTGACCGCATCTGTGA
  GTATAATCCGGTGGAAGTGTCCATGCTGACGTGTCTGGCTGATGTCCGGGAGCCTTGCCAGTTGGGCTGT
  ACAAACCTGACTTACTGTACAAACTTTAACAACAGGCCAACAGAACTGTTCAGGAGCTGCACGGCTCAGT
  CTGACCAGGGGGCCATGAGTGACATGAAGCTGTGGGAGAAGGGGAGCATCAAGATGCCCTTCATCAGCAT
  CCCTGTCCTAGACATCAAGACCTGCCAGCCAGAGATGTGGAAGGCCGTGGCCTGCTCCCTGCAGATCAAG
  CCGTGCCATAGCAAATCCCGAGGCAGTATTATCTGCAAATCAGATTGTGTAGAGATTCTCAAGAAATGTG
  GAGACCAGAATAAATTCCCTGAAGAACACACAGCTGAAAGTATCTGTGAGTTTCTGTCCCCTGCAGATGA
  CCTGGAGAGCTGTATCCCTCTGGACACGTACCTCAGGCCAAGTGCTTTAGGAAACATCATAGAAGAAGTG
  ACTCACCCCTGTAACCCAAACCCCTGCCCAGCCAACGAGCTCTGTGAAGTGAACCGGAAGGGGTGTCCGT
  CTGCAGATCCCTGCCTTCCATACTCCTGTGTTCAAGGCTGCAAATTGGGAGAAGCTTCTGACTTCATCGT
  CCGCCAGGGGACACTGATCCAGGTGCCGTCCTCTGCAGGGGAAGTTGGTTGCTACAAGATCTGTTCCTGT
  GGACAAAGTGGCCTCTTGGAAAACTGTATGGAAATGCACTGTATCGACCTCCAGAAGTCTTGTATTGTCG
  GAGGAAAAAGAAAAAGCCACGGAACATCCTTTACTATTGACTGCAATGTCTGTTCTTGTTTTGCGGGTAA
  TTTAGTGTGTTCCACCCGCCTGTGCCTCAGTGAGCACAGTTCAGACGACGACCGCCGCACCTTCACAGGT
  CTGCCCTGTAACTGCGCAGATCAGTTTGTCCCAGTGTGTGCACAGAATGGACGCACTTACCCCAGTGCCT
  GCATCGCTCGCTGTGTGGGTCTCCAGGACCATCAGTTTGAGTTTGGACCTTGTATCTCCAAGAATCCCTG
  TAATCCTAACCTCTGCCCCAAAAGCCAAAGATGTGTGCCAAAACCACAAGTCTGCCTGACGACTTTTGAT
  AAATTTGGATGTAGCCAATATGAGTGTGTGCCCAGACAGCTCACCTGTGACCAGGCCCGAGATCCTGTGT
  GTGACACAGATCACATGGAGCACAGCAACCTCTGCACTCTGTACCAAAGAGGGAAAAGCCTGTCTTACAG
  AGGCCCTTGCCAGCCTTTCTGCAGAGCCAAGGAGCCTGTCTGTGGGCACAATGGTGAGACATACAGCAGT
  GTGTGTGCTGCCTACTCAGATCGTGTGGCCGTGGATTACTACGGGCCCTGCCAGGCTGTCGGCGTCCTCT
  CGGAATACAGTGCTGTGGCTGAGTGTGCTGCTGTCAAGTGTCCTTCCCTCTCAGCCATAGGCTGCAAACC
  CATCATCCCACCAGGGGCTTGCTGTCCACTATGTGCTGGGATGTTACGGGTATTATTTGACAAAGAAAAA
  CTGGACACTATTGCTAAGGTGACAAGCAAGAAGCCCATAACCGTTGTGGAGATTCTTCAGAAGGTCCGCA
  TGCACGTGTCTGTGCCGCAGTGTGACGTGTTTGGATACTTGAGCATCGAGTCGGAAATTGTGATCCTCAT
  TATTCCTGTGGACCATTACCCAAAAGCTTTGCAGATTGAGGCCTGCAATAAGGAGGCAGAGAAAATCGAG
  TCCCTCATCAACTCCGACAGCCCCACGCTGGCGTCCCACGTCCCACTCTCGGCCCTCATCATTTCCCAAG
  TGCAGGTCTCCAGCAGTCTCCCGTCATCTGCTGTTGTGGGCAGGCCTCTCTTCCACTCCCTGCTCCTCCT
  CCTCAGCCTGGGCCTCACCGTGCACTTGCTCTGGACTAGACCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016678
• Insert Size: 2916 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016678.2, NP_057887.2
• RefSeq Size: 4450 bp
• RefSeq ORF: 2916 bp
• Locus ID: 53614
• UniProt ID: Q9Z0J1
• Cytogenetics: 4 B1
• Gene Summary: Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28803732). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:26658478, PubMed:28803732). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (By similarity). ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (By similarity). Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity (PubMed:11747814). Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP) (PubMed:11747814).[UniProtKB/Swiss-Prot Function]