Trpc4 (NM_016984) Mouse Untagged Clone

CAT#: MC222901

Trpc4 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 (Trpc4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpc4
• Synonyms: CCE1; STRPC4; Trp4; Trrp4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222901 representing NM_016984
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGTTCTATTACAAAAGAAATGTCAACGCCCCCTACAGAGACCGCATCCCACTGAGGATTGTCA
  GAGCAGAATCTGAGCTCTCACCATCAGAGAAAGCCTACTTGAATGCTGTGGAGAAGGGGGACTATGCAAG
  CGTCAAGAAGTCTCTGGAGGAAGCTGAGATTTATTTTAAAATCAACATTAACTGCATCGACCCCCTGGGA
  AGGACCGCCCTCCTCATTGCCATTGAAAATGAGAATCTGGAGCTTATTGAACTATTGTTGAGTTTCAATG
  TCTATGTAGGCGATGCGCTGCTTCACGCCATCAGAAAAGAGGTGGTTGGAGCCGTGGAGCTACTGCTGAA
  CCACAAAAAGCCAAGTGGAGAGAAGCAGGTGCCTCCCATTCTCCTTGATAAACAGTTCTCTGAATTCACT
  CCGGACATCACACCCATCATCTTGGCTGCACATACAAATAATTACGAGATAATCAAACTTTTGGTTCAGA
  AAGGTGTCTCAGTGCCCAGACCCCACGAGGTCCGCTGTAACTGTGTTGAGTGTGTCTCCAGCTCGGATGT
  GGACAGCCTCAGGCATTCACGGTCCAGGCTCAACATCTACAAGGCCTTGGCCAGCCCCTCGCTCATTGCC
  CTGTCAAGCGAAGACCCTTTCCTTACTGCCTTTCAGTTAAGTTGGGAGCTGCAAGAACTCAGCAAGGTGG
  AGAACGAATTCAAGTCGGAGTATGAGGAGCTGTCTAGACAGTGCAAACAATTTGCCAAGGACCTCCTAGA
  TCAGACACGGAGTTCCAGAGAGCTGGAAATCATTCTTAATTACCGTGATGACAATAGTCTGATCGAAGAA
  CAGAGTGGAAATGATCTTGCAAGGCTAAAATTAGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCTCAGC
  CCAACTGCCAGCAGCTGCTCGCTTCCCGCTGGTACGATGAGTTCCCAGGCTGGAGGAGAAGACACTGGGC
  GGTGAAGATGGTGACGTGTTTCATAATAGGACTACTCTTCCCCGTCTTCTCCGTGTGCTACCTGATAGCT
  CCCAAAAGCCCACTTGGACTGTTCATCAGAAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATC
  TGACCTTTTTGTTTCTGCTGCTGCTAGCCTCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCC
  ACCACCAACCATCGTGGAGTGGATGATATTACCGTGGGTCCTGGGTTTTATATGGGGAGAGATTAAACAG
  ATGTGGGATGGCGGACTCCAGGATTACATCCATGACTGGTGGAATCTAATGGACTTTGTGATGAACTCCT
  TGTATCTGGCAACAATCTCCTTGAAGATTGTCGCGTTTGTAAAGTACAGTGCTCTGAACCCACGGGAATC
  ATGGGACATGTGGCACCCCACCCTGGTGGCAGAGGCATTATTTGCTATTGCAAACATCTTCAGTTCCCTC
  CGCCTGATCTCTCTGTTCACTGCCAATTCTCACCTGGGGCCTCTGCAGATATCTCTGGGAAGGATGCTTC
  TGGACATCCTGAAGTTCTTGTTCATCTACTGCCTCGTGCTGCTAGCTTTTGCAAATGGCCTAAATCAGCT
  GTACTTTTACTATGAAGAAACAAAGGGGCTAAGCTGCAAAGGCATCCGGTGCGAGAAACAGAACAACGCG
  TTTTCCACGTTATTCGAGACACTACAGTCCCTGTTTTGGTCAATATTTGGACTCATCAATCTCTATGTTA
  CCAATGTCAAAGCCCAGCACGAGTTCACTGAGTTTGTTGGGGCCACCATGTTTGGCACATATAATGTCAT
  CTCTCTGGTTGTCCTGCTGAACATGTTAATTGCTATGATGAATAATTCTTACCAACTAATTGCCGACCAT
  GCAGATATAGAATGGAAATTTGCTCGAACAAAGCTTTGGATGAGCTACTTTGAAGAAGGAGGTACCCTGC
  CTACACCTTTCAATGTCATCCCAAGCCCCAAGTCCCTGTGGTACCTGGTCAAGTGGATATGGACACACTT
  ATGTAAGAAAAAAATGAGAAGGAAGCCAGAAAGCTTCGGGACAATTGGGCGGCGTGCTGCTGATAACTTG
  AGAAGACATCACCAATACCAAGAGGTGATGAGGAACCTGGTGAAGCGGTACGTGGCAGCCATGATCAGAG
  AGGCAAAAACCGAAGAAGGCTTGACGGAGGAGAATGTTAAGGAACTAAAGCAAGACATTTCTAGCTTCCG
  CTTCGAAGTTCTGGGATTGCTCAGAGGAAGCAAGCTCTCTACAATACAGTCAGCCAACGCGGCGAGTTCA
  GCGGACTCCGACGAGAAGAGCCAGAGCGAAGGTAATGGCAAGGACAAGAGAAAGAATCTCAGCCTCTTTG
  ATTTAACCACTCTGATCCACCCGCGGTCGGCAGCCATTGCCTCCGAGAGACATAACCTAAGCAATGGTTC
  CGCCCTGGTGGTGCAGGAGCCGCCCAGGGAGAAGCAGAGGAAAGTGAATTTTGTGGCTGATATCAAAAAC
  TTCGGGTTATTTCATAGACGGTCAAAACAAAATGCTGCTGAGCAAAACGCAAACCAAATCTTCTCTGTTT
  CAGAAGAAATTACTCGTCAACAGGCGGCAGGAGCACTTGAGCGAAATATCGAACTGGAATCCAAAGGATT
  AGCTTCACGGGGTGACCGCAGCATTCCTGGTCTCAATGAACAGTGTGTGCTAGTAGACCATAGAGAAAGG
  AATACGGACACTTTGGGTTTACAGGTAGGCAAGAGAGTGTGCTCCACCTTCAAGTCGGAGAAGGTGGTGG
  TGGAAGACACCGTCCCTATTATACCAAAGGAGAAACACGCCCACGAGGAGGACTCGAGCATAGACTATGA
  CTTAAGCCCCACGGACACAGCTGCCCACGAAGATTATGTGACCACAAGATTGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_016984
• Insert Size: 2925 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016984.3, NP_058680.1
• RefSeq Size: 3490 bp
• RefSeq ORF: 2925 bp
• Locus ID: 22066
• UniProt ID: Q9QUQ5
• Cytogenetics: 3 25.43 cM
• Gene Summary: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).