Pcdh8 (NM_001042726) Mouse Untagged Clone

CAT#: MC222897

Pcdh8 (untagged) - Mouse protocadherin 8 (Pcdh8), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcdh8
• Synonyms: 1700080P15Rik; P; Papc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222897 representing NM_001042726
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCCTGCGAAGCGCTGGGGCAGCCCTTGCCTTTTCCCCTTGCAGCTATTTAGTCTCTGCTGGGTGC
  TCTCAGTGGCCCAGAGCAAGACAGTCCGATACAGCACGTTCGAAGAGGATGCCCCTGGCACAGTCATCGG
  AACCCTTGCAGAAGACCTGCACATGAAAGTGTCTGGAGACACAAGCTTCCGCCTGATGAAGCAATTCAAC
  AGCTCTCTGCTCCGGGTACGCGAGGGCGACGGGCAGCTGACCGTCGGGGATGCGGGCCTGGACCGTGAGC
  GTCTGTGTGGCCCGTCCCCACAGTGCGTGCTGGCCTTCGATGTTGTCAGCTTCTCGCAGGAACAGTTCCG
  CCTGGTGCACGTGGAGGTGGAGGTGAGGGATGTAAACGACCACGCGCCCCGCTTTCCCCGAGCCCAGATC
  CCGGTGGAGGTCTCAGAGAGCGCTCCGGTAGGCACACGCATCCCCCTGGAGGTGCCCGTGGACGAGGACG
  TGGGTGCCAACGGGCTGCAGAGTGTACGCCTAGCCGAGCCTCACAGCCCCTTCCGCGTGGAGCTGCAGAC
  GCGCGCGGACGGTGCCCAGTGTGCGGACCTGGTGCTGCTACAGGAGTTGGACCGCGAGAGCCAGGCCTCC
  TACAGCCTGGAGCTGGTGGCTCAGGATGGCGGGCGACCGCCGCGTTCCGCCACAGCAGCCCTCAGCGTGC
  GTGTGCTAGATGCCAATGACCACAGCCCGGCCTTCCCACAGGGTGCCGTGGCAGAGGTGGAATTGGCCGA
  GGACGCACCTGTGGGATCGCTGCTGCTCGACCTGGACGCTGCCGATCCCGACGAAGGTCCCAACGGCGAC
  GTTGTGTTCACCTTCGGCGCCCGCACCCCTCCCGAAGCCCGCCACCTCTTCCGGCTCGACCCGCGCTCTG
  GCCGCCTCACCTTGGCTGGGCAGGTGGACTACGAGCGCCAGGACACCTACGAGCTGGACGTGCGGGCTCA
  AGACCGAGGTCCAGGGCCCCGGACAGCCACCTGCAAGGTTATCGTGCGCATCCGAGACGTCAACGACAAC
  GCTCCTGAAATTTCTATCACTCCTCTGGCCGCCCCGGGGGCTCCAGCCACCTCGCCCTTCGCCGCCGCCG
  CCGCTGCCGCCGCACTCGGAGGAGCGGACGCGGCCTCGTCGACTGGGTCCGGGACACAGGAAGCCGGTAT
  CACCTCGCTGGTGCCAGAGGGGGCGGCGCGCGAGAGTCTAGTGGCGCTGGTCAGCACCTCGGACAGGGAC
  TCGGGCGCCAACGGGCAGGTGCGCTGCGCCCTCTACGGGCACGAGCACTTTAGGCTGCAGCCGGCCTATG
  CTGGCAGCTACCTGGTGGTGACCGCTGCATCTCTGGACCGGGAGCGCATCGCGGAGTACAACCTGACGCT
  GGTGGCCGAAGACCGTGGCACACCCCCTCTTCGCACCGTCAGGCCCTACACCGTGCGAGTGGGGGACGAG
  AACGACAACGCACCGATCTTCACCAAGCCAGTCTATGAGGTGTCGGTCCGCGAAAACAACCCTCCAGGCG
  CCTACCTGGCCACAGTGGCAGCCCGCGACCCTGACGTGGGTCGCAACGGTCAAGTCACCTACCGCCTGGT
  AGAGGCCGAAGTGGGCCGCTCTGGGGAAGCCGTGTCCACTTACGTCTCAGTAGACCCGGCTACCGGAGCC
  ATCTACGCTCTCCGTAGTTTTGACTATGAGACTCTGCGCCAGCTTGACGTGCGTGTCCAGGCCAGCGACG
  GCGGTTCCCCTCAGCTTTCCAGCAATGCTCTGGTGCAAGTGCGGGTGCTGGACCAGAATGATCATTCTCC
  GATCCTTGTGCATCCGGCGCCGGCTAATGGCTCCCTAGAAGTAGCGGTCCCTGGACGTTCCACCAAGGAC
  ACAGCTGTGGCGCGCATCCAGGCCCGGGATGCTGACGAAGGAGCCAACGGGGAACTGGCCTTTGATCTGC
  TGCAGCAGGAGCCACGCGAGGCCTTCTCCATAGGCCGCCACACGGGGGAGATCATGCTTACTGGAGACCT
  CTCGCAGGAGCCTCCTGGCCGCGTGTTCAAGGCCCTGCTGGTCATATCCGACGGCGGCCGTCCCCCTCTC
  ACCACCACTGCAACCGTCAGTTTCGTGGTAACAGCCGGGGGCGGGTCAGCTGTGCCTGCCAGCTCCGGGA
  GCCCAGAGCACTCCCGGCCACCCGGCTCTCGCCTCGCGCCGTCGGGGCCTTCGCTGCAGTGGGACACGCC
  GCTGATCGTCATCATCGTGTTAGCCGGGAGCTGCACGCTGCTGCTTGCAGCCATCATCGCCATCGCCACC
  ACCTGCAACCGCCGCAAGAAGGAGCCTTACGGTGCCTCTCCAGGCTTCGGGAAGGAGCCTGCTGCGCCCC
  CTGTTGCAGTCTGGAAGGGTCATTCATTCAACACCATCTCGGGCCGAGAAGCTGAGAAGTTCAGTGGGAA
  AGACAGCGGCAAAGGAGACAGTGATTTCAATGACAGTGACTCGGACATCAGCGGGGACGCTTTGAAAAAA
  GACCTCATCAACCACATGCAGAGTGGACTGTGGGCGTGTACTGCTGAGTGCAAGATCCTAGGTCATTCTG
  ATCGCTGCTGGAGCCCATCCTGTGCCGGACCCAACGTACACCCCCCTCCTCACCCACCAGCCCAGATGTC
  AACCTTCTGTAAGAGCACGTCCCTGCCTCGGGATCCTCTGCGCAGGGACAATTACTATCAGGCCCAGCTC
  CCCAAGACAGTAGGGTTGCAGAGCGTCTATGAGAAAGTACTGCATAGAGACTATGACAGGACAGTCACTT
  TACTCTCCCCTCCCCGTCCAGGAAGGCTCCCAGACCTGCAGGAGATTGGAGTACCCCTCTATGAATCCCC
  TCCTGGTAGCAGATACGTGTCCCCGAAGAAGGGAATCAATGAAAATGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042726
• Insert Size: 2922 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042726.3, NP_001036191.1
• RefSeq Size: 3712 bp
• RefSeq ORF: 2922 bp
• Locus ID: 18530
• UniProt ID: Q7TSK3
• Cytogenetics: 14 42.76 cM
• Gene Summary: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a type I transmembrane protein composed of an extracellular domain including 6 cadherin ectodomains, a single-pass transmembrane domain and a cytoplasmic tail. Unlike classical cadherins, which are generally encoded by 15-17 exons, this gene includes only 3 exons with the first large exon encoding the extracellular and transmembrane region. Although this gene product is capable of homophilic interaction, it appears to affect cell-cell adhesion indirectly by initiating signaling events that regulate classical cadherin-mediated adhesion. Based on studies on this protein and its orthologs, this protocadherin mainly functions in developing embryos and the central nervous system, but can also function as a tumor suppressor. Alternative splicing yielding isoforms with unique cytoplasmic tails has been reported. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a protein (isoform 2) with a shorter cytoplasmic domain, compared to isoform 1.