Nfkb1 (NM_008689) Mouse Untagged Clone

CAT#: MC222894

Nfkb1 (untagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, p105 (Nfkb1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfkb1
• Synonyms: NF-kappaB; NF-kappaB1; NF-KB1; p50; p50/p105; p105
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222894 representing NM_008689
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACGATGATCCCTACGGAACTGGGCAAATGTTTCATTTGAACACTGCTTTGACTCACTCAATAT
  TTAATGCAGAATTATATTCACCAGAAATACCACTGTCAACAGATGGCCCATACCTTCAAATATTAGAGCA
  ACCAAAACAGAGGGGATTTCGATTCCGCTATGTGTGTGAAGGCCCATCACACGGAGGGCTTCCGGGAGCC
  TCTAGTGAGAAGAACAAGAAATCCTACCCACAGGTCAAAATTTGCAACTATGTGGGGCCTGCAAAGGTTA
  TCGTTCAGTTGGTCACAAATGGAAAAAACATCCACCTGCACGCCCACAGCCTGGTGGGCAAGCACTGTGA
  GGACGGGGTATGCACCGTAACAGCAGGACCCAAGGACATGGTGGTTGGCTTTGCAAACCTGGGAATACTT
  CATGTGACTAAGAAAAAGGTATTTGAAACACTGGAAGCACGGATGACAGAGGCGTGTATTAGGGGCTATA
  ATCCTGGACTTCTGGTGCATTCTGACCTTGCCTATCTACAAGCAGAAGGCGGAGGAGACCGGCAACTCAC
  AGACAGAGAGAAGGAGATCATCCGCCAGGCAGCCGTGCAGCAGACCAAGGAGATGGACCTGAGCGTGGTG
  CGCCTCATGTTCACAGCCTTCCTCCCTGACAGCACTGGCAGCTTCACTCGGAGACTGGAGCCTGTGGTGT
  CAGACGCCATCTATGATAGCAAAGCCCCGAATGCATCCAACCTGAAAATCGTGAGAATGGACAGAACAGC
  AGGATGTGTGACGGGAGGGGAGGAGATTTACCTTCTCTGTGACAAGGTTCAGAAAGATGACATCCAGATT
  CGGTTTTATGAAGAGGAAGAAAATGGCGGAGTTTGGGAAGGATTTGGGGACTTTTCCCCCACAGATGTTC
  ATAGACAGTTTGCCATTGTCTTCAAAACGCCAAAGTATAAGGATGTCAACATTACAAAGCCAGCTTCCGT
  GTTTGTTCAGCTTCGGAGGAAATCAGACCTGGAAACTAGTGAACCGAAACCCTTTCTCTACTACCCTGAA
  ATCAAAGACAAAGAGGAAGTGCAAAGGAAACGCCAGAAGCTTATGCCGAACTTCTCGGACAGCTTCGGTG
  GCGGCAGTGGAGCGGGAGCCGGTGGTGGAGGCATGTTCGGTAGTGGCGGTGGCGGAGGGAGTACCGGAAG
  CCCTGGCCCAGGGTATGGCTACTCGAACTACGGATTTCCTCCCTACGGTGGGATTACATTCCATCCCGGA
  GTCACGAAATCCAACGCAGGGGTCACCCATGGCACCATAAACACCAAATTTAAAAATGGCCCTAAAGATT
  GTGCCAAGAGTGATGACGAGGAGAGTCTGACTCTCCCTGAGAAGGAAACTGAAGGTGAAGGGCCCAGCCT
  GCCCATGGCCTGCACCAAGACGGAACCCATCGCCTTGGCATCCACCATGGAAGACAAGGAGCAGGACATG
  GGATTTCAGGATAACCTCTTTCTTGAGAAGGCTCTGCAGCTCGCCAGGCGACACGCCAACGCCCTTTTCG
  ACTACGCAGTGACGGGGGATGTGAAGATGTTGCTGGCCGTGCAACGCCATCTCACCGCCGTGCAGGATGA
  GAATGGGGACAGTGTCTTACACTTAGCCATCATCCACCTCCACGCTCAGCTTGTGAGGGATCTGCTGGAA
  GTCACATCTGGTTTGATCTCTGATGACATCATCAACATGAGAAATGACCTGTATCAGACACCTCTGCACT
  TGGCCGTGATCACCAAGCAGGAAGATGTAGTAGAGGATTTGCTGAGGGTTGGGGCTGACCTGAGCCTTCT
  GGACCGCTGGGGCAACTCTGTCCTGCACCTAGCTGCCAAAGAAGGACACGACAGAATCCTCAGCATCCTG
  CTCAAGAGCAGAAAAGCAGCGCCCCTTATCGACCACCCCAATGGGGAAGGTCTAAATGCCATCCACATAG
  CTGTGATGAGCAATAGCCTGCCATGTCTGCTGCTGCTGGTGGCTGCCGGGGCAGAAGTCAATGCTCAGGA
  GCAGAAGTCTGGGCGCACAGCGCTGCACCTGGCCGTGGAGTACGACAACATCTCCTTGGCTGGCTGCCTG
  CTTCTGGAGGGTGATGCCCACGTGGACAGTACCACCTATGATGGGACTACACCTCTGCATATAGCGGCCG
  GAAGAGGGTCCACCAGACTGGCAGCTCTTCTCAAAGCAGCAGGAGCAGACCCCCTGGTGGAGAACTTTGA
  GCCTCTCTATGACCTGGACGACTCTTGGGAGAAGGCTGGAGAAGATGAGGGAGTGGTGCCAGGTACCACA
  CCCCTGGACATGGCTGCCAACTGGCAGGTATTTGACATACTAAATGGGAAACCGTATGAGCCTGTGTTCA
  CATCTGATGATATACTACCACAAGGGGACATGAAGCAGCTGACAGAAGACACGAGGCTACAACTCTGCAA
  ACTGCTGGAAATTCCTGATCCAGACAAAAACTGGGCCACTCTGGCACAGAAGTTGGGTCTGGGGATACTG
  AACAATGCCTTCCGGCTGAGTCCTGCTCCTTCTAAAACTCTCATGGACAACTATGAGGTCTCTGGGGGTA
  CCATCAAAGAGCTGATGGAGGCCCTGCAACAGATGGGCTACACAGAGGCCATTGAAGTGATCCAGGCAGC
  CTTCCGCACCCCGGCAACCACAGCCTCCAGCCCCGTGACCACTGCTCAGGTCCACTGTCTGCCTCTCTCG
  TCTTCCTCCACGAGGCAGCACATAGATGAACTCCGGGATAGTGACAGCGTCTGTGACAGTGGTGTGGAGA
  CATCCTTCCGCAAACTCAGCTTTACAGAGTCTCTTACTGGAGACAGCCCACTGCTATCTCTGAACAAAAT
  GCCCCACGGTTATGGGCAGGAAGGACCTATTGAAGGCAAAATTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008689
• Insert Size: 2916 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008689.2, NP_032715.2
• RefSeq Size: 4128 bp
• RefSeq ORF: 2916 bp
• Locus ID: 18033
• UniProt ID: P25799
• Cytogenetics: 3 62.82 cM
• Gene Summary: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. Plays a role in the regulation of apoptosis. Isoform 5, isoform 6 and isoform 7 act as inhibitors of transactivation of p50 NF-kappa-B subunit, probably by sequestering it in the cytoplasm. Isoform 3 (p98) (but not p84 or p105) acts as a transactivator of NF-kappa-B-regulated gene expression. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.[UniProtKB/Swiss-Prot Function]