Ireb2 (NM_022655) Mouse Untagged Clone

CAT#: MC222876

Ireb2 (untagged) - Mouse iron responsive element binding protein 2 (Ireb2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ireb2
• Synonyms: D9Ertd85e; Irp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222876 representing NM_022655
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCCCAAGTGCAGGATACACCTTTGAGTACCTTATTGAAACATTAAATGGCAATTCACAGAAGA
  AGTTCTTCAATGTACCTAAACTTGGCGGCACCAAGTATGATATTCTGCCTTACTCAATACGGGTCCTATT
  GGAAGCTGCTGTACGAAATTGTGATGGCTTTTTAATGAAAAAGGAAGATGTTATGAACATTTTGGACTGG
  AAAACCAAACAGAGCAATGTTGAAGTGCCCTTTTTCCCTGCCCGTGTTGTTCTTCAAGATTTCACTGGAA
  TACCGGCAATGGTGGATTTTGCTGCTATGAGGGAGGCAGTGAAAACTCTTGGAGGTGATCCTAAGAAAGT
  CCACCCTGCCTGTCCGACAGATCTCACAGTGGACCACTCTTTACAGATTGACTTCAGTAAATGTGCAATA
  CAGAATGCACCAAATCCTGGAGGTGGTGACCTACAGAAAGCAGGAAAGCTCTCTCCACTTAAAGTGCAGT
  CTAAGAAGCTTCCATGTCGAGGCCAGACTACCTGCCGGGGATCGTGTGATTCTGGAGAGCTAAGCCGGAA
  CTCAGGAACATTTTCTTCACAGATTGAGAATACACCTGTCTTGTGTCCTTTTCATTTGCAACCAGTGCCT
  GAACCTGAGACAGTGTTAAAAAATCAAGAAGTAGAATTTGGCAGAAATCGAGAGAGGCTTCAGTTTTTCA
  AGTGGAGCTCAGGAGCTTTTAAGAATGTGGCAGTCATCCCGCCTGGAACTGGAATGGCTCATCAAGTGAA
  CTTAGAATATTTATCAAGAGTAGTTTTTGAAGAAACGGACCTGCTCTTCCCAGACAGTGTAGTTGGCACA
  GATTCTCATATAACCATGGTGAACGGATTGGGGATTCTTGGGTGGGGAGTTGGTGGCATTGAGACAGAGG
  CAGTTATGCTTGGCCTGCCAGTTACTCTTACTTTACCAGAGGTGGTTGGATGTGAGCTAACTGGGTCATC
  CAATGCTTTTGTTACATCCATAGATATTGTCCTTGGCATTACAAAGCACCTCAGGCAAGTAGGCGTGGCT
  GGAAAGTTTGTTGAGTTCTTTGGAAGTGGAGTTTCACAATTATCTATTGTCGATAGAACTACCATAGCAA
  ACATGTGTCCAGAATATGGTGCTATCCTCAGCTTTTTCCCTGTTGACAATGTGACACTACGACATTTAGA
  ACATACAGGTTTTGACAAAACCAAACTTGAGTCAATGGAAAAATACCTTAAAGCTGTGAAATTGTTTCGA
  AATGATGAGAATTCTTCAGAACCTGAATATTCTCAGGTGATACAGATTAATCTGAATTCAATAGTTGCAT
  CTGTCAGTGGTCCAAAAAGGCCTCAGGATAGGGTTGCTGTAACAGATATGAAAAGTGATTTTCAGGCTTG
  CTTAAATGAAAAGGTTGGATTTAAAGGCTTCCAAGTTGCAGCAGAAAAACAAAGTGACACTGTCTCTGTT
  CGTTATGATGGAAGTGAGTATAAGCTGTCCCATGGATCCGTGGTCATTGCTGCAGTTATCAGTTGTACCA
  ATAATTGCAATCCATCTGTGATGCTTGCTGCAGGTCTTCTGGCTAAAAAGGCTGTTGAAATTGGTCTACG
  AGTTAAACCTTATATACGAACAAGTTTGTCTCCAGGCAGTGGGATGGTTACACATTACCTCAGTTCAAGT
  GGAGTGCTACCCTATCTTAGCAAGCTAGGGTTTGACATAGTTGGCTATGGATGTTCAACATGTGTAGGAA
  ATACAGCACCTTTATCAGAAGCAGTTTTGAATGCAGTAAAACAGGGTGATTTGGTTACCTGTGGAGTTTT
  ATCTGGAAACAAACATTTCGAAGGCCGTCTCTGTGATTGTGTCCGTGCCAATTATCTCGCCTCTCCGCCC
  TTAGTGGTGGCTTATGCCATAGCAGGCACAGTGAATATAGACTTCCAGACAGAGCCTTTAGGTACTGACT
  CTACGGGCAAGGAAATTTACCTGCATGACATTTGGCCTAGTCGAGAAGAAGTTCATCAGATGGAGGAAGA
  ACATGTTATATTGTCAATGTTTAAAACACTAAAAGAGAAAGTAGAGATGGGAAATAAACGGTGGAATTCG
  TTAGAAGCTCCAGACTCCGTGCTGTTTCCATGGGATGTGAAGTCTACTTACATCAGATGCCCTTCATTTT
  TTGATAAGCTTACCAAAGAGCCAGCTGCATCCCAGCCTATTGAGAATGCCCATGTCCTGTTGTATTTGGG
  AGACTCTGTCACTACAGATCATATATCACCCGCTGGAAGTATTGCTAGGAGTAGTGCTGCTGCTAAGTAT
  TTGACAAACAGAGGCCTTACTCCCCGAGAGTTCAACTCTTATGGAGCTCGAAGAGGTAATGATGCTGTGA
  TGACAAGAGGCACATTTGCAAACATCAAGCTTTTTAATAAGTTTATTGGGAAGCCAGCTCCCAAAACAAT
  TCATTTTCCATCGGGACAAACGCTCGATGTATTTGAAGCTGCAGAACTGTACCAGAAAGAAGGTATCCCA
  CTGATAATTTTAGCAGGAAAAAAATATGGTTCAGGAAATTCAAGAGATTGGGCTGCCAAGGGACCCTATT
  TGCTGGGTGTAAAAGCTGTTTTGGCTGAGAGCTATGAAAAAATACACAAAGATCATTTGATTGGAATTGG
  TATAGCACCACTTGAATTCCTCCCAGGAGAAAATGCAGATTCCTTGGGCCTCTCTGGCAGAGAAGTATTT
  TCTTTATCATTTCCTGAAGAACTCTTTCCTGGAATTACATTAAATATAAAGACAAGCACTGGAAAAGAGT
  TCAGCGTGATTGCATCATTTGCAAATGACGTGGAGATAACTTTGTACAAACATGGAGGCTTATTAAACTT
  TGTGGCTCGAAAATTCTTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022655
• Insert Size: 2892 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022655.3, NP_073146.2
• RefSeq Size: 5808 bp
• RefSeq ORF: 2892 bp
• Locus ID: 64602
• UniProt ID: Q811J3
• Cytogenetics: 9 29.81 cM
• Gene Summary: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3' UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.[UniProtKB/Swiss-Prot Function]