Ptk2b (NM_172498) Mouse Untagged Clone

CAT#: MC222867

Ptk2b (untagged) - Mouse PTK2 protein tyrosine kinase 2 beta (Ptk2b), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptk2b
• Synonyms: CADTK; CAKB; CAKbeta; E430023O05Rik; FADK2; FAK2; PYK2; Raftk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222867 representing NM_172498
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGGTGTCTGAGCCCTTGAGCCGTGTAAAAGTGGGCACTTTACGCCGGCCTGAGGGCCCCCCAG
  AGCCCATGGTGGTGGTACCAGTGGATGTGGAGAAGGAAGACGTGCGCATCCTCAAGGTCTGCTTCTACAG
  CAACAGCTTCAACCCAGGGAAGAACTTCAAGCTTGTCAAATGCACAGTGCAGACAGAGATCCAGGAGATC
  ATCACCTCCATCCTCCTGAGTGGGCGAATAGGGCCCAACATCCAGCTGGCTGAATGCTATGGGCTGAGGC
  TGAAGCACATGAAGTCAGACGAGATCCACTGGCTGCACCCACAGATGACCGTGGGCGAAGTGCAGGACAA
  GTATGAATGTCTACACGTGGAAGCTGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCGGAAGACTTC
  ATGGAGAGCCTGAAAGAAGACAGGACCACATTGCTGTACTTTTATCAACAGCTCCGGAATGACTACATGC
  AACGCTACGCCAGCAAGGTCAGTGAAGGCATGGCTCTGCAGCTGGGCTGTCTGGAGCTCAGGAGATTCTT
  CAAGGACATGCCCCACAATGCACTGGACAAAAAGTCCAACTTTGAACTCCTGGAAAAAGAAGTCGGTCTG
  GACCTGTTTTTCCCAAAGCAGATGCAGGAAAACTTAAAGCCCAAGCAGTTCCGGAAGATGATCCAGCAGA
  CCTTCCAGCAGTATGCATCACTCCGGGAGGAAGAGTGTGTCATGAAATTCTTCAATACCCTAGCGGGCTT
  TGCCAACATTGACCAGGAGACCTACCGCTGCGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTC
  ATCGGCCCTAAAGGCATCCGTCAGCTGACAAGTCAAGATACAAAGCCCACCTGCCTGGCCGAGTTTAAGC
  AGATCAAATCCATCAGGTGCCTCCCATTGGAAGAGACCCAGGCAGTCCTGCAGCTGGGCATCGAGGGTGC
  CCCCCAGTCCTTGTCTATCAAAACGTCGTCCCTGGCAGAGGCTGAGAACATGGCTGACCTCATAGATGGC
  TACTGCAGGCTGCAAGGAGAACATAAGGGCTCTCTCATCATGCATGCCAAGAAAGATGGTGAGAAGAGGA
  ACAGCCTGCCTCAGATCCCCACACTAAACCTGGAGGCTCGGCGGTCGCACCTCTCAGAAAGCTGCAGCAT
  AGAGTCAGACATCTATGCGGAGATTCCCGATGAGACCCTGCGAAGACCAGGAGGTCCACAGTACGGTGTT
  GCCCGTGAAGAAGTAGTTCTTAACCGCATTCTGGGTGAAGGCTTCTTTGGGGAGGTCTATGAAGGTGTCT
  ACACGAACCACAAAGGGGAAAAAATTAATGTGGCCGTCAAGACCTGTAAGAAAGACTGTACCCAGGACAA
  CAAGGAGAAGTTCATGAGTGAGGCAGTGATCATGAAGAATCTTGACCACCCTCACATCGTGAAGCTGATT
  GGCATCATTGAAGAGGAACCCACCTGGATTATCATGGAACTGTATCCTTATGGGGAGCTGGGACACTACC
  TGGAACGAAATAAAAACTCCCTGAAGGTACCCACTCTGGTCCTGTACACCCTACAGATATGCAAAGCCAT
  GGCCTATCTGGAGAGCATCAACTGTGTGCACAGGGATATTGCTGTCCGGAACATCCTGGTGGCCTCTCCT
  GAGTGTGTGAAGCTGGGGGACTTTGGGCTCTCCCGGTACATTGAGGACGAAGACTATTACAAAGCCTCTG
  TGACCCGTCTACCCATCAAATGGATGTCCCCCGAGTCCATCAACTTCCGCCGCTTCACAACCGCCAGTGA
  TGTCTGGATGTTTGCTGTATGCATGTGGGAGATCCTCAGCTTTGGGAAGCAGCCTTTCTTCTGGCTCGAA
  AATAAGGATGTCATCGGAGTGCTGGAGAAAGGGGACAGGCTGCCCAAGCCCGAACTCTGTCCGCCTGTCC
  TTTACACACTCATGACTCGCTGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTCACGGAGCTTGTGTG
  CAGCCTCAGTGACATTTATCAGATGGAGAAGGACATTGCCATAGAGCAAGAAAGGAATGCTCGCTACCGA
  CCCCCCAAAATATTGGAGCCTACTACCTTTCAGGAACCCCCACCCAAGCCCAGCCGGCCCAAGTACAGAC
  CTCCTCCACAGACCAACCTGCTGGCTCCTAAGCTGCAGTTCCAGGAGGAGGACTTCATCCGGCCCAGTAG
  CCGAGAAGAGGCCCAGCAGCTCTGGGAGGCAGAGAAGATCAAGATGAAGCAGGTCCTAGAAAGACAGCAG
  AAGCAGATGGTGGAAGATTCCCAGTGGCTGAGGCGAGAGGAAAGATGCTTGGACCCTATGGTTTATATGA
  ATGACAAGTCCCCACTGACTCCAGAGAAGGAGGCCGGCTACACGGAGTTCACAGGGCCCCCACAGAAACC
  ACCTCGGCTCGGTGCACAGTCCATTCAGCCCACAGCCAACCTGGACAGGACCGATGACCTCGTGTACCAC
  AATGTCATGACCCTGGTGGAGGCTGTGCTGGAACTCAAGAACAAGCTTGGCCAGTTGCCCCCTGAGGACT
  ATGTGGTGGTGGTGAAGAACGTGGGGCTGAACCTGCGGAAGCTCATCGGCAGTGTGGACGATCTCTTGCC
  CTCCTTGCCGGCATCTTCGAGGACAGAGATTGAAGGGACCCAGAAACTGCTCAACAAAGACCTGGCAGAG
  CTCATCAACAAGATGAAGTTGGCTCAGCAGAACGCCGTGACGTCCCTGAGTGAGGACTGCAAGCGGCAGA
  TGCTCACAGCGTCCCATACCCTGGCTGTGGATGCCAAGAACCTGCTGGATGCTGTGGACCAAGCCAAGGT
  TGTGGCTAATCTGGCCCACCCGCCTGCAGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172498
• Insert Size: 2904 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172498.3, NP_766086.2
• RefSeq Size: 3925 bp
• RefSeq ORF: 2904 bp
• Locus ID: 19229
• Cytogenetics: 14 34.36 cM
• Gene Summary: Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376' (By similarity). Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. This results in a shorter protein (isoform 3) compared to isoform 1.