Kif5b (NM_008448) Mouse Untagged Clone

CAT#: MC222865

Kif5b (untagged) - Mouse kinesin family member 5B (Kif5b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kif5b
• Synonyms: AL022807; Khc; Khcs; Kns1; Ukhc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222865 representing NM_008448
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCCGGCGGAGTGCAACATCAAAGTGATGTGTCGCTTCAGACCTCTCAACGAATCTGAAGTGA
  ACCGCGGCGATAAGTACGTCGCCAAATTCCAGGGAGAAGACACGGTGATGATCGCGTCCAAGCCTTATGC
  CTTTGATCGTGTGTTCCAGTCAAGCACATCTCAAGAGCAAGTATACAACGACTGTGCAAAAAAGATTGTT
  AAAGATGTTCTTGAGGGCTATAATGGAACAATATTTGCATATGGACAAACATCATCTGGGAAGACCCACA
  CGATGGAGGGTAAACTTCATGATCCAGAAGGCATGGGAATTATTCCAAGAATAGTGCAAGATATTTTTAA
  TTATATTTACTCCATGGATGAAAATTTGGAATTTCATATTAAGGTTTCATATTTTGAAATATATTTGGAT
  AAGATAAGGGACTTGTTAGATGTTTCAAAGACTAACCTTTCAGTCCATGAAGACAAAAACCGTGTTCCCT
  ATGTAAAGGGGTGCACAGAACGTTTCGTGTGTAGTCCAGATGAAGTCATGGATACCATAGATGAAGGGAA
  ATCCAACAGACATGTCGCAGTTACAAATATGAATGAACATAGCTCTAGGAGTCACAGCATATTTCTTATT
  AATGTAAAACAAGAGAATACACAAACGGAACAGAAACTCAGTGGAAAGCTTTATCTGGTTGATTTAGCTG
  GCAGTGAGAAGGTTAGTAAGACTGGGGCTGAAGGTGCTGTGCTGGATGAAGCTAAGAACATCAACAAGTC
  ACTTTCTGCACTTGGAAATGTCATTTCTGCTTTGGCAGAGGGCAGTACCTATGTTCCTTATCGAGATAGT
  AAAATGACCAGAATTCTTCAAGATTCATTAGGTGGCAACTGTAGGACCACTATTGTCATATGCTGCTCTC
  CATCATCATACAATGAGTCTGAGACAAAGTCAACACTCCTCTTTGGTCAAAGGGCCAAAACAATTAAGAA
  CACAGTCTGTGTCAATGTAGAGTTAACTGCGGAGCAGTGGAAAAAGAAGTATGAAAAAGAAAAGGAAAAA
  AATAAAACTCTACGGAACACTATTCAGTGGCTGGAAAACGAGCTAAACCGTTGGCGTAACGGGGAGACAG
  TGCCTATTGATGAGCAGTTTGACAAAGAGAAAGCTAATTTGGAAGCCTTCACAGCGGATAAAGATATTGC
  TATTACCAGTGATAAACCAGCTGCTGCAGTCGGAATGGCTGGTAGTTTTACCGATGCTGAAAGAAGAAAG
  TGTGAAGAAGAACTTGCTAAATTGTATAAACAACTTGATGACAAGGATGAAGAGATTAACCAACAAAGCC
  AATTGGTAGAGAAATTGAAGACACAAATGCTGGATCAGGAAGAGCTTCTGGCATCAACCAGAAGGGATCA
  AGATAATATGCAAGCTGAACTGAATCGCCTCCAAGCAGAAAATGATGCTTCTAAAGAAGAAGTCAAAGAA
  GTTTTACAGGCCTTAGAGGAACTGGCTGTTAATTATGATCAGAAGTCTCAGGAAGTTGAAGACAAAACAA
  AGGAATATGAATTGCTTAGTGATGAATTGAATCAAAAATCTGCAACTTTAGCAAGTATTGATGCTGAGCT
  TCAGAAGCTGAAGGAAATGACCAACCACCAGAAGAAACGAGCAGCTGAAATGATGGCATCATTATTAAAA
  GACCTTGCAGAAATAGGAATTGCTGTGGGGAATAACGATGTGAAGCAACCAGAAGGAACTGGTATGATAG
  ATGAAGAGTTTACTGTTGCAAGACTCTACATTAGCAAAATGAAATCAGAAGTAAAGACCATGGTGAAACG
  CTGCAAACAGCTAGAAAGCACACAGACTGAGAGCAACAAAAAAATGGAAGAAAATGAGAAAGAGTTAGCA
  GCATGCCAGCTTCGGATCTCCCAACATGAAGCCAAAATCAAGTCACTGACTGAGTACCTTCAGAATGTAG
  AACAAAAGAAGAGGCAGCTGGAGGAGTCTGTTGATTCCCTTGGTGAGGAGCTAGTCCAACTCCGAGCACA
  AGAGAAAGTCCATGAAATGGAAAAAGAGCACTTGAACAAGGTTCAGACTGCAAATGAAGTCAAGCAAGCT
  GTTGAGCAGCAGATCCAGAGTCACAGAGAAACCCACCAAAAACAAATCAGTAGTTTGCGAGATGAAGTGG
  AGGCAAAGGAAAAGCTAATCACTGACCTCCAAGACCAAAACCAGAAGATGGTGTTGGAGCAGGAACGGCT
  AAGGGTGGAGCATGAGAGGCTGAAGGCTACAGACCAAGAGAAGAGCAGGAAGCTGCACGAGCTCACGGTT
  ATGCAAGACAGACGAGAACAAGCAAGACAAGACTTGAAGGGTTTGGAGGAGACCGTGGCAAAAGAACTTC
  AGACTTTACACAACCTGCGTAAGCTCTTTGTTCAGGACTTGGCTACCAGGGTGAAAAAGAGCGCCGAGGT
  CGACTCTGACGACACTGGCGGCAGTGCTGCACAGAAGCAGAAAATCTCCTTCCTTGAAAACAACCTTGAA
  CAGCTCACCAAAGTGCACAAGCAGTTGGTACGCGATAATGCAGATCTTCGCTGTGAGCTTCCTAAGTTAG
  AGAAACGGCTTAGAGCTACTGCAGAAAGAGTGAAAGCTTTGGAGTCAGCCCTGAAAGAAGCCAAAGAAAA
  TGCATCTCGAGACCGTAAACGCTATCAGCAAGAAGTAGACCGGATAAAGGAAGCAGTCAGGTCAAAGAAC
  ATGGCCAGAAGGGGACATTCTGCCCAGATTGCAAAGCCGATCCGTCCTGGACAGCATCCAGCGGCCTCGC
  CAACTCACCCGGGCACAGTTCGTGGAGGAGGCTCATTTGTTCAGAACAACCAGCCAGTGGGGCTTCGTGG
  TGGTGGAGGCAAGCAGTCGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008448
• Insert Size: 2892 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008448.3, NP_032474.2
• RefSeq Size: 6030 bp
• RefSeq ORF: 2892 bp
• Locus ID: 16573
• UniProt ID: Q61768
• Cytogenetics: 18 4.46 cM
• Gene Summary: Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. May be involved in the mechanisms of growth arrest induced by exposure to DNA-damaging drugs or by cellular senescence (PubMed:9657148). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (PubMed:21976701). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).[UniProtKB/Swiss-Prot Function]