Ganab (NM_008060) Mouse Untagged Clone

CAT#: MC222863

Ganab (untagged) - Mouse alpha glucosidase 2 alpha neutral subunit (Ganab), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ganab
• Synonyms: AU042638; G2an; GluII; mKIAA0088
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222863 representing NM_008060
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAATAGCGGCAGTGGCGGCGCGTAGGAGGCGGTCTTGGTTAAGTTTGGTGCTGGCATACTTAG
  GGGTCTGTCTGGGGATTACACTTGCTGTGGATAGAAGCAACTTTAAGACCTGTGATGAGAGTTCCTTTTG
  CAAACGGCAGCGAAGCATTCGGCCAGGCCTCTCTCCTTACCGTGCCTTGCTGGACACTCTGCAGCTTGGT
  CCTGATGCTCTTACAGTCCATCTGATCCATGAAGTCACCAAGGTGCTGCTTGTGCTGGAGCTCCAGGGCC
  TTCAGAAGAACATGACTCGGATCAGGATCGATGAGCTAGAGCCCCGGCGGCCTCGATACCGAGTGCCAGA
  TGTTTTAGTGGCTGACCCCCCCACAGCTAGGCTTTCAGTCTCTGGCCGTGATGACAACAGTGTGGAGCTA
  ACAGTGGCTGAGGGACCCTACAAAATCATTTTGACAGCACAGCCATTCCGCCTTGACCTGCTAGAAGATC
  GAAGCCTCCTGCTCAGTGTCAATGCCCGAGGACTTATGGCCTTTGAGCACCAGAGGGCCCCCAGGGTCCC
  TTTCTCGGATAAAGTTAGTCTCGCGCTCGGTAGCGTGTGGGATAAGATCAAGAACCTTTTCTCTAGGCAA
  GAATCAAAAGACCCAGCTGAAGGCAATGGAGCCCAGCCTGAAGCAACACCTGGGGATGGTGACAAGCCAG
  AGGAGACCCAGGAAAAGGCTGAGAAGGATGAGCCAGGAGCCTGGGAGGAGACATTCAAAACACATTCTGA
  CAGCAAGCCTTATGGCCCCACGTCTGTAGGTTTGGACTTTTCTCTGCCAGGAATGGAACATGTGTATGGG
  ATCCCTGAGCATGCTGACAGCCTGAGACTGAAGGTCACTGAGGGCGGTGAGCCGTACCGCCTGTACAATT
  TGGATGTGTTCCAGTATGAGCTGAACAACCCCATGGCTCTATATGGGTCTGTGCCTGTGCTCCTGGCACA
  CAGCTTTCATCGAGACCTGGGCATCTTCTGGCTTAATGCTGCTGAGACTTGGGTTGATATATCCTCCAAC
  ACGGCTGGGAAGACCCTGTTTGGGAAGATGCTTGATTACCTGCAGGGCTCTGGGGAGACTCCACAGACAG
  ACATTCGTTGGATGTCAGAGAGTGGCATTATTGATGTTTTCCTAATGCTTGGCCCTTCGGTTTTTGATGT
  CTTTAGGCAGTATGCTAGTCTCACAGGGACCCAGGCATTGCCCCCACTCTTCTCCCTCGGCTATCACCAG
  AGTCGCTGGAACTACCGGGATGAGGCTGATGTTTTGGAAGTGGATCAGGGTTTTGATGATCACAACATGC
  CTTGTGATGTCATTTGGTTGGACATTGAACATGCTGATGGCAAGCGGTACTTCACTTGGGACCCCACCCG
  ATTTCCTCAGCCCCTCAATATGCTTGAGCACTTGGCTTCCAAGAGGCGGAAGCTGGTGGCCATTGTGGAC
  CCCCACATCAAGGTAGACTCTGGCTACCGAGTTCACGAAGAATTGCGAAACCATGGGCTGTATGTTAAAA
  CTCGGGATGGCTCTGATTACGAGGGCTGGTGCTGGCCAGGCTCAGCTAGTTACCCTGACTTCACTAATCC
  AAGGATGAGGGCCTGGTGGTCTAACATGTTCAGCTTTGACAATTATGAGGGTTCAGCTCCTAATCTTTAT
  GTTTGGAATGACATGAATGAACCGTCTGTGTTCAATGGTCCTGAGGTCACCATGTTGAAGGATGCTGTGC
  ATTATGGAGGCTGGGAGCACCGGGACATCCATAACATCTATGGCTTATATGTGCACATGGCGACTGCTGA
  TGGGCTAATACAGCGCTCTGGGGGCATAGAGCGTCCCTTTGTCCTGAGTAGGGCTTTCTTCTCAGGCTCC
  CAGCGCTTTGGAGCTGTGTGGACAGGGGACAACACTGCCGAATGGGATCATTTGAAGATCTCTATCCCTA
  TGTGTCTCAGCCTGGCACTGGTGGGGCTTTCCTTCTGTGGAGCGGATGTGGGTGGCTTCTTCAAGAACCC
  AGAGCCAGAGCTGCTTGTGCGCTGGTACCAAATGGGTGCCTACCAGCCGTTCTTTCGGGCTCATGCCCAC
  TTGGACACTGGGCGGCGAGAGCCATGGCTGTTAGCGTCTCAATACCAAGATGCAATCCGAGATGCCTTGT
  TCCAGCGATATTCTTTGCTGCCCTTCTGGTATACCCTCTTCTATCAAGCTCACAAGGAAGGGTTTCCTGT
  CATGAGGCCCCTCTGGGTACAGTATCCTGAGGATATGTCTACCTTCAGTATAGAGGATCAGTTCATGCTT
  GGTGATGCACTCCTTATTCACCCTGTATCGGATGCTGGGGCCCACGGAGTGCAGGTCTATTTGCCTGGCC
  AAGAAGAAGTGTGGTATGACATTCAGAGCTATCAGAAGCATCATGGGCCCCAGACCTTGTATCTGCCAGT
  AACTTTGAGCAGTATCCCCGTGTTCCAGCGTGGCGGAACCATTGTGCCTCGATGGATGCGTGTGAGGCGC
  TCTTCAGACTGTATGAAGGACGATCCTATCACTCTCTTTGTTGCTCTCAGTCCCCAGGGTACTGCCCAAG
  GAGAGCTCTTTCTAGATGATGGACACACCTTTAACTATCAGACTCGCCATGAGTTCCTGTTGCGGCGGTT
  CTCTTTCTCTGGCAGCACACTGGTCTCTAGTTCAGCAGACCCCAAAGGCCACCTTGAGACACCTATTTGG
  ATTGAGCGAGTAGTCATCATGGGGGCTGGAAAGCCAGCAGCTGTGGTGCTCCAGACCAAAGGATCCCCTG
  AAAGTCGCCTGTCCTTCCAGCATGACCCTGAGACCTCAGTGCTGATATTGCGTAAACCTGGTGTCAGCGT
  GGCATCCGACTGGAGTATTCATCTTCGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008060
• Insert Size: 2901 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008060.2, NP_032086.1
• RefSeq Size: 3974 bp
• RefSeq ORF: 2901 bp
• Locus ID: 14376
• UniProt ID: Q8BHN3
• Cytogenetics: 19 A
• Gene Summary: Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed:27462106). Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia (PubMed:28375157).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).