Rasip1 (NM_028544) Mouse Untagged Clone

CAT#: MC222836

Rasip1 (untagged) - Mouse Ras interacting protein 1 (Rasip1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rasip1
• Synonyms: 1110025D03Rik; 2610025P08Rik; AI853551; Rain
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222836 representing NM_028544
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTATCTGGTGAACGAAAGGAGGGCGGAAGCCCCCGCTTCGGGAAGCTCCATCTTCCAGTGGGCCTGT
  GGATCAATTCCCCAAGGAAACAGCTCGCCAAACTGGGGCGGCGCTGGCCCAGCGCTGCCTCTGTCAAGTC
  CTCGTCCTCCGACACGGGGAGCCGAAGCAGCGAGCCCCTGCCGCCGCCGCCGCCGCCGCCGCACGTGGAG
  CTGCGGCGCGTAGGTGCGGTGAAGGCGGCGGGGGGAGCGTCGGGCAGCCGAGCCAAGCGCATCTCGCAGC
  TGTTTCGGGGCTCAGGGGCCGGCGGCGCCGGGGGTCCTGGCACGCCTGGGGGCGCGCAGCGCTGGGCCAG
  CGAGAAGAAGCTGCCTGAACTGGCGGCGGGTGTAGCTCCGGAGCCCCCGTTGCCCACGCGTGCCGCGGTG
  CCCCCGGGAGTGCTCAAGATCTTCGCTTCTGGGCTGGCGTCGGGCGCCAACTACAAGAGCGTGCTGGCTA
  CGGAGCGATCCACCGCGCGCGAGCTGGTGGCGGAGGCGCTGGAGCGCTACGGGCTGACGGGCGGCCGCGG
  AGCGGGCGACAGCGGCTGCGTGGACGCCTACGCGCTTTGCGACGCTCTGGGCCGGCCCGCGGTCGGCGTG
  GGCGGAGGCGAGTGGCGTGCCGAGCACCTGCGGGTGTTGGCCGACGCGGAGCGCCCTCTGCTGGTGCAGG
  ACCTGTGGCGCGCCCGTCCGGGCTGGGCGCGGCGCTTTGAGCTGCGCGGCCGCGAGGAGGCTCGACGGCT
  GGAGCAGGAGGCCTTCGGGGCTGCGGATGCAGACGGCACAAACGCCCCATCTTGGCGGACCCAGAAGAAT
  AGGTCTCGGGCAGCATCTGGCGGGGCAGCTCTGGCCAGTCCTGGTCCAGGATCAGGATCCGGGACCCCCA
  CTGGGTCCGGGGGCAAGGAGCGATCTGAAAATTTGTCTCTGCGTCGAAGCGTGTCTGAGCTGAGTCTACA
  GGGGCGGAGGCGAAGGCAGCAGGAACGCAGGCAGCAGGCCCTTAGCATGGCCCCAGGGGCAGCTGATGCT
  CAGATGGTACCAACGGACCCTGGAGACTTCGATCAGTTGACTCAGTGCCTCATTCAGGCACCCAGCAACC
  GTCCCTACTTCCTTCTGCTCCAGGGCTACCAGGATGCTCAGGACTTCGTGGTGTATGTCATGACACGAGA
  GCAGCACGTGTTCGGCCGGGGTGGGCCCTCCTCGTCCCGCGGGGGATCCCCAGCCCCATATGTGGACACC
  TTCCTCAACGCCCCAGACATCCTGCCTCGACATTGCACGGTGCGCGCGGGCCCTGAGCCCCCGGCCATGG
  TGCGCCCATCCCGGGGCGCCCCCGTCACACACAATGGCTGTCTTTTGCTGCGGGAGGCAGAGCTGCACCC
  CGGAGACCTGTTAGGCCTAGGAGAGCATTTCCTGTTCATGTACAAGGACCCCCGCTCCGGGGGTTCTGGG
  CCCGCTCGGCCATCTTGGCTGCCAGCGCGCCCCGGGGCCGCGCCACCCGGACCGGGCTGGGCCTTTTCTT
  GTCGTTTGTGTGGCCGTGGCCTGCAGGAGCGCGGCGAGGCGCTGGCCGCCTACCTGGATGGCCGCGAGCC
  GGTGCTGCGGTTCAGACCACGAGAGGAAGAGGCGCTGTTGGGCGAGATCGTGCGAGCTGCAGCCTCGGGA
  GCAGGGGACCTGCCCCCGCTCGGGCCAGCCACTCTGCTGGCACTGTGTGTGCAGCATTCTGCGAGGGAGC
  TGGAGCTGGGCCACCTGCCTCGCTTGCTGGGCCGCCTGGCCCGGCTCATCAAAGAAGCCGTTTGGGAAAA
  AATTAAAGAAATTGGAGACCGCCAGCCAGAGAACCACCCAGAGGGAGTTCCCGAGGTGCCTCTGACCCCT
  GAGGCTGTGTCTGTGGAGCTGCGACCACTCATACTATGGATGGCCAACACCACAGAGCTGTTGAGCTTTG
  TACAGGAGAAAGTGCTAGAGATGGAGAAGGAGGCTGACCAGGAGGGCCTGTCCTCGGACCCACAGCTCTG
  CAATGACTTGGAACTGTGTGATGAGGCCTTGGCCCTCCTGGATGAGGTCATCATGTGTACTTTCCAGCAG
  TCGGTCTACTACCTCACCAAGACTCTTTATTCAACACTGCCAGCTCTCCTGGACAGTAACCCTTTCACAG
  CTGGAGCAGAGCTGCCAGGGCCTGGCGCAGAGCTGGAGGCCATGCCGCCTGGGCTCAGGCCTACTCTGGG
  TGTGTTCCAGGCAGCTCTAGAACTGACCAGTCAGTGTGAGCTGCATCCCGACCTCGTGTCCCAGACTTTT
  GGCTATTTGTTCTTCTTCTCCAATGCATCCCTTCTCAACTCACTGATGGAACGAGGTCAAGGCCGACCTT
  TCTATCAATGGTCTCGAGCCGTCCAAATCAGAACCAACTTGGATCTTGTGTTGGATTGGCTTCAGGGGGC
  CGGGCTGGGTGACATTGCCACTGAGTTCTTCCGGAAACTTTCCATTGCTGTGAACCTGCTATGTGTGCCC
  CGCACCTCCCTGCTCAAGGCTTCATGGAGCAGCTTACGGACTGACTACCCCACCCTGACTCCAGCTCAGC
  TCCACCATCTCCTCAGCCACTACCAGTTGGGTCCTGGCCGTGGTCCCCCACCAGCCTGGGACCCACCACC
  TGCAGAGCGGGATGCTGTGGACACAGGGGATATCTTTGAAAGTTTTTCCTCGCACCCTCCGCTCATCCTG
  CCCCTGGGCAGCTCACGCCTGCGCCTCACCGGCCCTGTGACAGATGACGCATTGCATAGAGAACTGCGCA
  GGCTCCGTCGCCTCCTCTGGGATCTTGAACAGCAGGAGCTGCCAGCCAATCATCGCCATGGACCCCCGGT
  GGCTTCGACACCTTGA
  
  CAAGCTTAACTAGCTAGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATC
  TGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAAAC
• Restriction Sites: SgfI-HindIII
• ACCN: NM_028544
• Insert Size: 2886 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028544.1, NP_082820.1
• RefSeq Size: 3169 bp
• RefSeq ORF: 2886 bp
• Locus ID: 69903
• UniProt ID: Q3U0S6
• Cytogenetics: 7 B3
• Gene Summary: Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells. May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function]