Gabbr1 (NM_019439) Mouse Untagged Clone

CAT#: MC222832

Gabbr1 (untagged) - Mouse gamma-aminobutyric acid (GABA) B receptor, 1 (Gabbr1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gabbr1
• Synonyms: bM573K1.1; GABAB1; GABAbR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222832 representing NM_019439
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGCTGCTGCTGGTGCCTCTCTTCCTCCGCCCCCTGGGCGCTGGCGGGGCTCAGACCCCCAACG
  TCACCTCGGAAGGTTGCCAGATTATACATCCGCCCTGGGAAGGTGGCATCAGGTACCGTGGCTTGACTCG
  CGACCAGGTGAAGGCCATCAATTTCCTGCCTGTGGACTATGAGATTGAATATGTGTGCCGGGGCGAACGC
  GAGGTGGTGGGGCCCAAGGTGCGCAAGTGCCTGGCCAACGGCTCCTGGACGGATATGGACACACCCAGTC
  GCTGTGTCCGAATCTGCTCCAAGTCTTATTTGACCCTGGAAAATGGGAAGGTTTTCCTGACGGGTGGGGA
  CCTCCCAGCTCTGGATGGAGCCCGGGTGGATTTCCGATGTGACCCCGACTTCCATCTGGTGGGCAGCTCC
  CGGAGCATCTGTAGTCAGGGCCAGTGGAGCACCCCCAAGCCCCACTGCCAGGTGAATCGAACGCCACACT
  CAGAACGGCGTGCAGTATACATCGGGGCGCTGTTTCCCATGAGCGGGGGCTGGCCGGGGGGCCAGGCCTG
  CCAGCCTGCGGTGGAGATGGCGCTGGAGGACGTTAACAGCCGCAGAGACATCCTGCCGGACTACGAGCTC
  AAGCTTATCCACCACGACAGCAAGTGCGACCCAGGGCAAGCCACCAAGTACTTGTATGAACTACTCTACA
  ACGACCCCATCAAGATCATCCTCATGCCCGGCTGCAGCTCTGTGTCCACACTGGTAGCCGAGGCTGCCCG
  GATGTGGAACCTTATTGTGCTCTCATATGGCTCCAGCTCACCAGCCTTGTCAAACCGACAGCGGTTTCCA
  ACGTTCTTTCGGACACATCCATCCGCCACACTCCACAATCCCACCCGGGTGAAACTCTTCGAAAAGTGGG
  GCTGGAAGAAGATTGCCACCATCCAGCAGACTACCGAGGTCTTCACCTCAACACTGGATGACCTGGAGGA
  GCGAGTGAAAGAGGCTGGGATTGAGATCACTTTTCGACAGAGTTTCTTCTCAGATCCAGCTGTGCCTGTT
  AAAAACCTGAAGCGTCAAGATGCTCGAATCATCGTGGGACTTTTCTATGAGACCGAAGCCCGGAAAGTTT
  TTTGTGAGGTCTATAAGGAACGGCTCTTTGGGAAGAAGTATGTCTGGTTTCTCATCGGGTGGTATGCTGA
  CAACTGGTTCAAAACCTATGACCCGTCAATCAATTGTACAGTAGAAGAGATGACTGAGGCGGTGGAGGGC
  CATATCACCACGGAGATTGTCATGCTGAACCCTGCCAACACCCGAAGCATTTCCAACATGACATCACAGG
  AATTTGTGGAGAAACTAACCAAGCGGCTGAAAAGACACCCTGAGGAGACTGGAGGCTTCCAGGAGGCACC
  ACTGGCCTATGATGCTATCTGGGCCTTGGCTTTGGCCTTGAACAAGACCTCTGGAGGAGGTGGCCGTTCA
  GGAGTGCGCCTGGAGGACTTTAACTACAACAACCAGACCATTACAGACCAAATCTACCGGGCCATGAACT
  CCTCCTCCTTTGAGGGTGTTTCTGGCCACGTGGTCTTTGATGCCAGCGGCTCCCGGATGGCATGGACGCT
  TATCGAGCAGCTACAGGGCGGCAGCTACAAGAAGATCGGCTACTACGACAGCACCAAGGATGATCTTTCC
  TGGTCCAAAACAGACAAGTGGATCGGAGGGTCTCCCCCAGCCGACCAGACCTTGGTCATCAAGACATTCC
  GTTTCCTGTCACAGAAACTCTTTATCTCCGTCTCAGTTCTCTCCAGCCTGGGCATTGTTCTTGCTGTTGT
  CTGTCTGTCCTTTAACATCTACAACTCCCACGTTCGTTATATCCAGAACTCCCAGCCCAACCTGAACAAT
  CTGACTGCTGTGGGCTGCTCACTGGCACTAGCTGCTGTCTTCCCCCTTGGGCTGGATGGTTACCACATAG
  GGAGAAGCCAGTTCCCATTTGTCTGCCAGGCCCGCCTTTGGCTCTTGGGCTTAGGCTTTAGTCTGGGCTA
  TGGCTCTATGTTCACCAAGATCTGGTGGGTCCACACAGTCTTCACAAAGAAGGAGGAGAAGAAGGAATGG
  AGGAAGACCCTAGAGCCTTGGAAACTGTACGCCACTGTAGGCCTGCTGGTGGGCATGGATATCCTGACTC
  TTGCCATCTGGCAGATTGTGGACCCCTTGCACCGAACCATTGAGACTTTTGCCAAGGAGGAACCAAAGGA
  AGACATTGATGTCTCCATTCTGCCCCAGCTGGAGCACTGCAGCTCCAAGAAGATGAATACATGGCTCGGC
  ATTTTCTATGGTTACAAAGGGCTGCTGCTGCTGCTGGGAATCTTTCTTGCTTATGAGACCAAAAGTGTGT
  CCACTGAAAAGATCAATGACCACAGGGCCGTGGGCATGGCTATCTACAATGTTGCGGTCCTGTGTCTCAT
  TACCGCTCCTGTCACCATGATCCTTTCCAGCCAGCAGGACGCAGCCTTCGCCTTTGCCTCTCTGGCCATT
  GTGTTCTCTTCCTACATTACTCTGGTTGTGCTCTTTGTGCCTAAGATGCGCAGGTTGATCACTCGAGGTG
  AATGGCAGTCTGAAGCACAGGACACAATGAAAACAGGATCATCCACCAACAACAATGAGGAAGAGAAGTC
  CCGACTGTTGGAGAAGGAAAACCGTGAATTGGAAAAGATCATCGCCGAGAAAGAGGAGCGTGTCTCTGAA
  CTGCGCCATCAGCTCCAGTCTCGGCAGCAGATCCGCTCCCGGCGCCACCCCCCAACACCCCCAGACCCCT
  CTGGGGGCCTTCCCAGGGGACCCTCAGAGCCCCCTGACCGGCTTAGCTGTGATGGGAGTCGAGTACATTT
  GCTTTACAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019439
• Insert Size: 2883 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019439.3, NP_062312.3
• RefSeq Size: 4486 bp
• RefSeq ORF: 2883 bp
• Locus ID: 54393
• UniProt ID: Q9WV18
• Cytogenetics: 17 19.16 cM
• Gene Summary: Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:10773016, PubMed:10075644). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10773016, PubMed:10075644). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Calcium is required for high affinity binding to GABA (By similarity). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (By similarity). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:10075644). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity).[UniProtKB/Swiss-Prot Function]