Phc3 (NM_001165955) Mouse Untagged Clone

CAT#: MC222814

Phc3 (untagged) - Mouse polyhomeotic-like 3 (Drosophila) (Phc3), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phc3
• Synonyms: E030046K01Rik; Edr3; Hph3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222814 representing NM_001165955
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGTGAGCCGAGCTCTGGAACATCCGTGTCAACAACAGCCAGCAGTACCACCACCACCACCATCA
  CCACATCCTCCTCCCGAATGCAGCAGCCACAGATCTCTGTCTACAGCGGGTCAGACCGACATGCTGTTCA
  GGTAATTCAGCAGGCCTTGCATCGGCCCCCCAGCTCGGCAGCTCAGTACCTGCAGCAGATGTACGCGGCT
  CAGCAGCAGCACCTGATGCTGCACACGGCTGCTCTGCAGCAGCAGCACCTAAGCAGCTCGCAGCTCCAGA
  GCCTTGCGGCTGTCCAGGCAAGTTTGTCCAGTGGAAGACCATCTACATCCCCCACAGGCAGTGTTACACA
  ACAGTCAAGTATGTCCCAGACATCTATCCTGTCCGCTTCTCCTGCACCTGCTCAGCTGATGAACCGCTCC
  CAGACGTCCAGTTCTACCAGCGGCAGTATTACCCAGCAGACTATGCTACTCGGGAGTACCTCTCCCACCC
  TAACTGCCAGCCAAGCCCAGATGTATCTCCGAGCTCAGATGGTTCAGAATTTAACCTTACGCAGCCAGAA
  GCTGGGTGTCTTATCTAGCTCACAGAATGGCTCACCAAAAAGCGCTGGTCAAACCCAGTCCTTGACAATT
  TGCCATAATAAAACAACAGTGACCAGTTCTAAAATCAGCCAACGAGACCCTTCTCCAGAGAGCAAGAAGG
  GAGGAAGCCCAGGTCTGGAATCTCGGAGCACGGCCGTCACTCGGACATCAAGCATCCACCAGTTAATAGC
  ACCAGCTTCATATTCTCCAATTCAGCCTCATTCTCTAATCAAACATCAGCAGATTCCTCTTCATTCACCA
  CCTCCTAAAGTTTCCCATCATCAGCTGCTATTACAACAGCAACAACAGCAAATTCAGCCAATCACCCTTC
  AGAGTCCAAGTCAAGATCCACCCCCATCCCAGCACTGTATCCCACTCCCAAACCATGGCCTTTCTCCGGC
  TCCCAGTAATGCCCAGCCGCAGCATTGCTCCCCAGTTCAGAGCCATCCTCCGCCTTTAACTGTGTCTCCC
  AACCAGGCACAGTCCGCACAGCAGTCTGTAGTGGTGTCTCCTCCCCCCCCTCATTCCCCAAGTCAGTCTC
  CTACTATAATTATCCATCCACAAGCACTTATCCAGCCACACCCTCTTGTGTCTTCAGCTCTTCAAACAGG
  GCCAAATTTGCAGCAGGCTGCTGCTGATCAGGTACAGTCCACCGCACAGCTGAATCTTCCATCCCATCTT
  CCACTTCCAGCATCCCCTGTTGTACACATTGGCCCTGTCCAGCAGTCTGCCTTGGTGTCCCCTGGTCAGC
  AGATGGTGTCCCCAACATCACACCAGCAGTATTCAGCCCTACAGTCCTCTCCAATTCCCATCGCGACCCC
  TCCACAGATGTCGGCATCTCCTCCAGCTCAGCTCCCACCACTGCCCTTGCAGTCTATGCAGTCTTTACAA
  GTGCAGCCTGAAATTCTATCCCAGGGCCAGGTTTTGGTGCAGAATGCTTTGGTGTCAGAAGAGGAGCTAC
  CTGCTGCAGAAGCTTTGGTCCAGTTGCCATTTCAGACTCTTCCTCCTCCACAGACTGTTGCGGTAAATCT
  ACAAGTACAGCCGCCAGCACCTGTTGATCCACCAGTGGTTTATCAAGTAGAAGATGTGTGTGAGGAAGAA
  ATGCCAGAGGAGTCCGATGAGTGTGCAAGGATGGACAGAACCCCCCCACCCCCCACCTTGTCTCCAGCAG
  CTGTCACTGTGGGGAGGGGAGAGGACTTGACATCCGAACATCCTTTGTTAGAGCAAGTGGAGTTGCCTGC
  TGTGGCCTCAGTCAGTGCTTCCGTGATCAAGTCTCCATCAGACCCCACCCATGCCTCTGCTCCTGCACCT
  CCCCTCTTAATCCCTGCTGCCTCCACCAGGAGCAGCAGCACTTCTCTGGCCAGCAGCACTCCCAGCCTGG
  AAAACAAGCCTCCACAGGCTATTGTGAAACCACAGATTTTGACCCACGTTATAGAAGGCTTTGTGATTCA
  GGAGGGGCTGGAGCCGTTTCCTGTGAGTCGATCATCACTGCTGATAGAACAACCTGTGAAGAAGAGGCCT
  CTCTTGGATAATCAGGTGGTAAACTCTGTGTGTGTCCAGCCAGAGTTACAGAATAACACAAAGCATGCAG
  ATAACTCATCCGACACAGAGATAGAAGACATGATGGCTGAAGAGACATTGGAAGAAATGGATAGCGAGTT
  ACTCAAGTGTGAATTCTGTGGAAAAATGGGATATCCTAATGAATTTTTACGGTCCAAACGATTCTGTACT
  ATGTCATGTGCCAAAAGGTACAACGTTAGCTGTTCTAAAAAATTTGCACTTAGTCGTTGGAATCGTAAGC
  CTGATAATCAGAGTCTTGGGCATCGGGGCCGTCGTCCAAGTGGCCCTGAAGGGGCAGCAAGAGAACATAT
  CCTTAGGCAGCTTCCAATTACTTATCCATCTGCAGAAGAAGACGTGGCCTCTCATGAAGATCCTGTGCCT
  TCTGCTATGACGACCCGTCTGCGAAGGCAGAGCGAGCGGGAAAGAGAGCGAGAGCTTCGGGATGTGAGGA
  TTAGGAAGATGCCTGAGAATAGTGACTTGCTCCCAGTCGCACAGACTGAGCCCTCCATATGGACAGTCGA
  CGACGTCTGGGCCTTCATCCATTCTTTGCCCGGCTGCCAGGACGTTGCAGATGAGTTCAGGGCACAAGAG
  ATTGATGGGCAGGCCCTGCTCTTGCTAAAAGAAGACCATCTCATGAGTGCAATGAACATGAAGCTGGGCC
  CAGCCCTGAAGATTTGTGCACGCATCAACTCTCTGAAGGACTCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001165955
• Insert Size: 2847 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165955.1, NP_001159427.1
• RefSeq Size: 10967 bp
• RefSeq ORF: 2847 bp
• Locus ID: 241915
• Cytogenetics: 3 A3
• Gene Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, uses a downstream translational start codon, and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.